Mouse/Rat/Porcine/Canine IGF-II/IGF2 Quantikine ELISA Kit

• Reactivity: Mu, Rt, Po, Ca
• Applications: ELISA
• Conjugate: HRP

Mouse/Rat/Porcine/Canine IGF-II/IGF2 Quantikine ELISA Kit Summary

• Background: The Quantikine® Mouse/Rat/Porcine/Canine IGF-II Immunoassay is a 3.5 hour solid-phase ELISA designed to measure IGF-II in serum-free cell culture supernates and serum. It contains E. coli-expressed recombinant IGF-II and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural IGF-II sho...wed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural IGF-II.
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• Specificity: Natural and recombinant IGF-II.
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse/Rat/Porcine/Canine IGF-II/IGF2 Quantikine ELISA Kit

• C11orf43
• chromosome 11 open reading frame 43
• FLJ22066
• FLJ44734
• GRDF
• IGF2
• IGF-2
• IGFII
• IGF-II
• insulin-like growth factor 2 (somatomedin A)
• insulin-like growth factor II
• insulin-like growth factor type 2
• MSA
• PEG2
• PP9974
• somatomedin-A

Background

Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity.

The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide and methanol, which are known to the State of California to cause cancer and reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.

Publications for IGF-II/IGF2 (MG200)(12)

Publications using MG200

• Courtney, Y;Head, JP;Dani, N;Chechneva, OV;Shipley, FB;Zhang, Y;Holtzman, MJ;Sadegh, C;Libermann, TA;Lehtinen, MK; Choroid plexus apocrine secretion shapes CSF proteome during mouse brain development Nature neuroscience 2025-05-28 [PMID: 40437054] (Mouse)
• Endo Kumata, Y;Inagaki, A;Nakamura, Y;Imura, T;Saito, R;Katano, T;Suzuki, S;Tokodai, K;Kamei, T;Unno, M;Watanabe, K;Tabata, Y;Goto, M; A novel method of pancreatic islet transplantation at the liver surface using a gelatin hydrogel nonwoven fabric Cell transplantation 2025-04-23 [PMID: 40264358] (Rat)
• Jang, HJ;Min, HY;Kang, YP;Boo, HJ;Kim, J;Ahn, JH;Oh, SH;Jung, JH;Park, CS;Park, JS;Kim, SY;Lee, HY; Tobacco-induced hyperglycemia promotes lung cancer progression via cancer cell-macrophage interaction through paracrine IGF2/IR/NPM1-driven PD-L1 expression Nature communications 2024-06-08 [PMID: 38851766] (Mouse)
• Guerra-Cantera, S;Frago, LM;Jiménez-Hernaiz, M;Collado-Pérez, R;Canelles, S;Ros, P;García-Piqueras, J;Pérez-Nadador, I;Barrios, V;Argente, J;Chowen, JA; The metabolic effects of resumption of a high fat diet after weight loss are sex dependent in mice Scientific reports 2023-08-14 [PMID: 37580448] (Mouse)
• Tu, X;Jain, A;Parra Bueno, P;Decker, H;Liu, X;Yasuda, R; Local autocrine plasticity signaling in single dendritic spines by insulin-like growth factors Science advances 2023-08-02 [PMID: 37531435] (Mouse)
• Kanai, N;Inagaki, A;Nakamura, Y;Imura, T;Mitsugashira, H;Saito, R;Miyagi, S;Watanabe, K;Kamei, T;Unno, M;Tabata, Y;Goto, M; A gelatin hydrogel nonwoven fabric improves outcomes of subcutaneous islet transplantation Scientific reports 2023-07-24 [PMID: 37488155] (Mouse)
• Saito, R;Inagaki, A;Nakamura, Y;Imura, T;Kanai, N;Mitsugashira, H;Endo, Y;Katano, T;Suzuki, S;Tokodai, K;Kamei, T;Unno, M;Watanabe, K;Tabata, Y;Goto, M; Ideal Duration of Pretreatment Using a Gelatin Hydrogel Nonwoven Fabric Prior to Subcutaneous Islet Transplantation Cell transplantation 2023-07-19 [PMID: 37466120] (Mouse)
• K Yamanaka, Y Haraguchi, H Takahashi, I Kawashima, T Shimizu Development of serum-free and grain-derived-nutrient-free medium using microalga-derived nutrients and mammalian cell-secreted growth factors for sustainable cultured meat production Scientific Reports, 2023-01-10;13(1):498. 2023-01-10 [PMID: 36627406] (Bovine)
• T Suzuki, Y Sato, S Kadoya, T Takahashi, M Otomo, H Kobayashi, K Aoki, M Kantake, M Sugiyama, RP Ferraris Comparative Effects of Allulose, Fructose, and Glucose on the Small Intestine Nutrients, 2022-08-07;14(15):. 2022-08-07 [PMID: 35956407] (Rat)
• AS Naik, SQ Wang, M Chowdhury, J Aqeel, CL O'Connor, JE Wiggins, M Bitzer, RC Wiggins Critical timing of ACEi initiation prevents compensatory glomerular hypertrophy in the remaining single kidney Scientific Reports, 2021-10-01;11(1):19605. 2021-10-01 [PMID: 34599260] (Rat)
• S Chen, J Jing, Y Yuan, J Feng, X Han, Q Wen, TV Ho, C Lee, Y Chai Runx2+ Niche Cells Maintain Incisor Mesenchymal Tissue Homeostasis through IGF Signaling Cell Rep, 2020-08-11;32(6):108007. 2020-08-11 [PMID: 32783935] (Mouse)
• Y Sui, W Zhang, T Tang, L Gao, T Cao, H Zhu, Q You, B Yu, T Yang Insulin-like growth factor-II overexpression accelerates parthenogenetic stem cell differentiation into cardiomyocytes and improves cardiac function after acute myocardial infarction in mice Stem Cell Res Ther, 2020-02-26;11(1):86. 2020-02-26 [PMID: 32102690] (Mouse)

FAQs for IGF-II/IGF2 (MG200). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.