Mouse/Rat PDGF-AB Quantikine ELISA Kit

• Reactivity: Mu, Rt
• Applications: ELISA
• Conjugate: HRP

Mouse/Rat PDGF-AB Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse/Rat PDGF-AB Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse/rat PDGF-AB in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat PDGF-AB and antibodies raised against recombinant rat PDGF. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse.../rat PDGF-AB showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse/rat PDGF-AB.
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• Specificity: Natural and recombinant mouse and rat PDGF-AB
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse/Rat PDGF-AB Quantikine ELISA Kit

• PDGFAB
• PDGF-AB

Background

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for PDGF-AB (MHD00)(10)

Publications using MHD00

• Okuno, N;Yamamoto, S;Hamashima, T;Dang, TS;Okita, N;Fujikawa, M;Kunisawa, T;Takakura, N;Fujimori, T;Mori, H;Betsholtz, C;Takata, K;Sasahara, M; Brain macrophages and pial fibroblasts promote inflammation in a hypomyelination model Acta neuropathologica communications 2025-07-04 [PMID: 40615895] (Transgenic Mouse)
• JD Koerner, DZ Markova, GD Schroeder, BP Calio, A Shah, CW Brooks, AR Vaccaro, DG Anderson, CK Kepler The local cytokine and growth factor response to rhBMP-2 after spinal fusion Spine J, 2018-03-14;0(0):. 2018-03-14 [PMID: 29550606] (ELISA (Standard), Rat)
• Adiponectin enhances bone marrow mesenchymal stem cell resistance to flow shear stress through AMP-activated protein kinase signaling Sci Rep, 2016-07-15;6(0):28752. 2016-07-15 [PMID: 27418435] (Rat)
• Schledzewski K, Geraud C, Arnold B, Wang S, Grone HJ, Kempf T, Wollert KC, Straub BK, Schirmacher P, Demory A, Schonhaber H, Gratchev A, Dietz L, Thierse HJ, Kzhyshkowska J, Goerdt S Deficiency of liver sinusoidal scavenger receptors stabilin-1 and -2 in mice causes glomerulofibrotic nephropathy via impaired hepatic clearance of noxious blood factors. J. Clin. Invest., 2011-02-01;121(2):703-14. 2011-02-01 [PMID: 21293057] (Mouse)
• Oku H, Shimizu T, Kawabata T, Nagira M, Hikita I, Ueyama A, Matsushima S, Torii M, Arimura A Antifibrotic action of pirfenidone and prednisolone: different effects on pulmonary cytokines and growth factors in bleomycin-induced murine pulmonary fibrosis. Eur. J. Pharmacol., 2008-06-16;590(1):400-8. 2008-06-16 [PMID: 18598692] (Mouse)
• Kilian O, Wenisch S, Karnati S, Baumgart-Vogt E, Hild A, Fuhrmann R, Jonuleit T, Dingeldein E, Schnettler R, Franke RP Observations on the microvasculature of bone defects filled with biodegradable nanoparticulate hydroxyapatite. Biomaterials, 2008-05-23;29(24):3429-37. 2008-05-23 [PMID: 18501961] (Porcine)
• Paintlia AS, Paintlia MK, Singh AK, Singh I Inhibition of rho family functions by lovastatin promotes myelin repair in ameliorating experimental autoimmune encephalomyelitis. Mol. Pharmacol., 2008-01-31;73(5):1381-93. 2008-01-31 [PMID: 18239032] (Rat)
• He M, Kubo H, Ishizawa K, Hegab AE, Yamamoto Y, Yamamoto H, Yamaya M The role of the receptor for advanced glycation end-products in lung fibrosis. Am. J. Physiol. Lung Cell Mol. Physiol., 2007-10-19;293(6):L1427-36. 2007-10-19 [PMID: 17951314] (Mouse)
• Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy. Proc. Natl. Acad. Sci. U.S.A., 2007-10-17;104(43):17069-74. 2007-10-17 [PMID: 17942672] (Mouse)
• Panoskaltsis-Mortari A, Tram KV, Price AP, Wendt CH, Blazar BR A new murine model for bronchiolitis obliterans post-bone marrow transplant. Am. J. Respir. Crit. Care Med., 2007-06-15;176(7):713-23. 2007-06-15 [PMID: 17575098] (Mouse)

FAQs for PDGF-AB (MHD00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.