Mouse/Rat Flt-3 Ligand/FLT3L Quantikine ELISA Kit

• Reactivity: Mu, Rt
• Applications: ELISA
• Conjugate: HRP

Mouse/Rat Flt-3 Ligand/FLT3L Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse/Rat Flt-3 Ligand Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat Flt-3 Ligand levels in cell culture supernates and serum. It contains NS0-expressed recombinant mouse Flt-3 Ligand and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse or rat Flt-3 Ligand showed linear curves that were parallel to... the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse/rat Flt-3 Ligand.
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• Specificity: Natural and recombinant mouse Flt-3 Ligand and natural rat Flt-3 Ligand
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Flt3l

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse/Rat Flt-3 Ligand/FLT3L Quantikine ELISA Kit

• FL
• FLG3L
• Flt3 ligand
• Flt-3 Ligand
• Flt3L
• FLT3LG
• fms-related tyrosine kinase 3 ligand
• SL cytokine

Background

Flt-3 Ligand, also known as FL, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Mature human Flt-3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. Within the ECD, human Flt-3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt-3 Ligand, respectively. Human and mouse Flt-3 Ligand show cross-species activity. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates. Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain. Alternate splicing of human Flt-3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine domain. Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation. Animal studies also show Flt-3 Ligand to reduce the severity of experimentally induced allergic inflammation.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Flt-3 Ligand/FLT3L (MFK00)(24)

Publications using MFK00

• Holmes-Hampton, GP;Kumar, VP;Biswas, S;Stone, S;Sharma, NK;Legesse, B;Vercellino, J;Guha, C;Eichenbaum, G;Ghosh, SP; PEGylated thrombopoietin mimetic, JNJ?26366821 a novel prophylactic radiation countermeasure for acute radiation injury Scientific reports 2023-09-14 [PMID: 37709916] (Mouse)
• T Fukaya, T Uto, S Mitoma, H Takagi, Y Nishikawa, M Tominaga, N Choijookhu, Y Hishikawa, K Sato Gut dysbiosis promotes the breakdown of oral tolerance mediated through dysfunction of mucosal dendritic cells Cell Reports, 2023-04-25;42(5):112431. 2023-04-25 [PMID: 37099426] (Mouse)
• A Tassou, M Thouaye, D Gilabert, A Jouvenel, JP Leyris, C Sonrier, L Diouloufet, I Mechaly, S Mallié, J Bertin, M Chentouf, M Neiveyans, M Pugnière, P Martineau, B Robert, X Capdevila, J Valmier, C Rivat Activation of neuronal FLT3 promotes exaggerated sensorial and emotional pain-related behaviors facilitating the transition from acute to chronic pain Progress in neurobiology, 2023-01-13;0(0):102405. 2023-01-13 [PMID: 36646299] (Mouse)
• J Zhu, Y Ke, Q Liu, J Yang, F Liu, R Xu, H Zhou, A Chen, J Xiao, F Meng, L Yu, R Li, J Wei, B Liu Engineered Lactococcus lactis secreting Flt3L and OX40 ligand for in situ vaccination-based cancer immunotherapy Nature Communications, 2022-12-03;13(1):7466. 2022-12-03 [PMID: 36463242] (Bacteria - Lactococcus lactis)
• VP Kumar, S Biswas, GP Holmes-Ham, M Sheleg, S Stone, B Legesse, R Ofir, SP Ghosh Pre-Administration of PLX-R18 Cells Protects Mice from Radiation-Induced Hematopoietic Failure and Lethality Genes, 2022-09-28;13(10):. 2022-09-28 [PMID: 36292639] (Mouse)
• VP Kumar, GP Holmes-Ham, S Biswas, S Stone, NK Sharma, B Hritzo, M Guilfoyle, G Eichenbaum, C Guha, SP Ghosh Mitigation of total body irradiation-induced mortality and hematopoietic injury of mice by a thrombopoietin mimetic (JNJ-26366821) Scientific Reports, 2022-03-03;12(1):3485. 2022-03-03 [PMID: 35241733] (Mouse)
• S Elhag, C Stremmel, A Zehrer, J Plocke, R Hennel, M Keuper, C Knabe, J Winterhalt, V Gölling, L Tomas, T Weinberger, M Fischer, L Liu, F Wagner, M Lorenz, K Stark, H Häcker, M Schmidt-Su, U Völker, M Jastroch, K Lauber, T Straub, B Walzog, E Hammer, C Schulz Differences in Cell-Intrinsic Inflammatory Programs of Yolk Sac and Bone Marrow Macrophages Cells, 2021-12-17;10(12):. 2021-12-17 [PMID: 34944072] (Mouse)
• VN Kapoor, S Müller, S Keerthivas, M Brown, C Chalouni, EE Storm, A Castiglion, R Lane, M Nitschke, CX Dominguez, JL Astarita, AT Krishnamur, CB Carbone, Y Senbabaogl, AW Wang, X Wu, V Cremasco, M Roose-Girm, L Tam, J Doerr, MZ Chen, WP Lee, Z Modrusan, YA Yang, R Bourgon, W Sandoval, AS Shaw, FJ de Sauvage, I Mellman, C Moussion, SJ Turley Gremlin 1+ fibroblastic niche maintains dendritic cell homeostasis in lymphoid tissues Nature Immunology, 2021-04-26;22(5):571-585. 2021-04-26 [PMID: 33903764] (Mouse)
• N Chakrabort, A Gautam, GP Holmes-Ham, VP Kumar, S Biswas, R Kumar, D Hamad, G Dimitrov, AO Olabisi, R Hammamieh, SP Ghosh microRNA and Metabolite Signatures Linked to Early Consequences of Lethal Radiation Sci Rep, 2020-03-25;10(1):5424. 2020-03-25 [PMID: 32214144] (Mouse)
• A Kim, S Shim, MJ Kim, JK Myung, S Park Mesenchymal stem cell-mediated Notch2 activation overcomes radiation-induced injury of the hematopoietic system Sci Rep, 2018-06-18;8(1):9277. 2018-06-18 [PMID: 29915190] (Mouse)
• Dolence JJ, Gwin K, Frank E, Medina KL Threshold levels of Flt3-ligand are required for the generation and survival of lymphoid progenitors and B-cell precursors. Eur. J. Immunol., 2011-02-01;41(2):324-34. 2011-02-01 [PMID: 21268003] (Mouse)
• Eidenschenk C, Crozat K, Krebs P, Arens R, Popkin D, Arnold CN, Blasius AL, Benedict CA, Moresco EM, Xia Y, Beutler B Flt3 permits survival during infection by rendering dendritic cells competent to activate NK cells. Proc. Natl. Acad. Sci. U.S.A., 2010-05-10;107(21):9759-64. 2010-05-10 [PMID: 20457904] (Mouse)
• Hochweller K, Miloud T, Striegler J, Naik S, Hammerling GJ, Garbi N Homeostasis of dendritic cells in lymphoid organs is controlled by regulation of their precursors via a feedback loop. Blood, 2009-09-18;114(20):4411-21. 2009-09-18 [PMID: 19767511] (Mouse)
• Horiuchi K, Morioka H, Takaishi H, Akiyama H, Blobel CP, Toyama Y Ectodomain shedding of FLT3 ligand is mediated by TNF-alpha converting enzyme. J. Immunol., 2009-06-15;182(12):7408-14. 2009-06-15 [PMID: 19494263] (Mouse)
• Li L, Piloto O, Nguyen HB, Greenberg K, Takamiya K, Racke F, Huso D, Small D Knock-in of an internal tandem duplication mutation into murine FLT3 confers myeloproliferative disease in a mouse model. Blood, 2008-02-01;111(7):3849-58. 2008-02-01 [PMID: 18245664] (Mouse)
• Triccas JA, Shklovskaya E, Spratt J, Ryan AA, Palendira U, Fazekas de St Groth B, Britton WJ Effects of DNA- and Mycobacterium bovis BCG-based delivery of the Flt3 ligand on protective immunity to Mycobacterium tuberculosis. Infect. Immun., 2007-08-27;75(11):5368-75. 2007-08-27 [PMID: 17724075] (Mouse)
• Tan K, Cheang P, Ho IA, Lam PY, Hui KM Nanosized bioceramic particles could function as efficient gene delivery vehicles with target specificity for the spleen. Gene Ther., 2007-03-08;14(10):828-35. 2007-03-08 [PMID: 17344903] (Mouse)
• Aiello FB, Keller JR, Klarmann KD, Dranoff G, Mazzucchelli R, Durum SK IL-7 induces myelopoiesis and erythropoiesis. J. Immunol., 2007-02-01;178(3):1553-63. 2007-02-01 [PMID: 17237404] (Mouse)
• Vollstedt S, O'Keeffe M, Ryf B, Glanzmann B, Hochrein H, Suter M The long-term but not the short-term antiviral effect of IFN-alpha depends on Flt3 ligand and pDC. Eur. J. Immunol., 2006-05-01;36(5):1231-40. 2006-05-01 [PMID: 16639711] (Mouse)
• Curtin JF, King GD, Barcia C, Liu C, Hubert FX, Guillonneau C, Josien R, Anegon I, Lowenstein PR, Castro MG Fms-like tyrosine kinase 3 ligand recruits plasmacytoid dendritic cells to the brain. J. Immunol., 2006-03-15;176(6):3566-77. 2006-03-15 [PMID: 16517725] (Rat)
• Ali S, Curtin JF, Zirger JM, Xiong W, King GD, Barcia C, Liu C, Puntel M, Goverdhana S, Lowenstein PR, Castro MG Inflammatory and anti-glioma effects of an adenovirus expressing human soluble Fms-like tyrosine kinase 3 ligand (hsFlt3L): treatment with hsFlt3L inhibits intracranial glioma progression. Mol. Ther., 2004-12-01;10(6):1071-84. 2004-12-01 [PMID: 15564139] (Rat)
• Manfra DJ, Chen SC, Jensen KK, Fine JS, Wiekowski MT, Lira SA Conditional expression of murine Flt3 ligand leads to expansion of multiple dendritic cell subsets in peripheral blood and tissues of transgenic mice. J. Immunol., 2003-03-15;170(6):2843-52. 2003-03-15 [PMID: 12626534] (Mouse)
• Miller G, 107331, Pillarisetty VG, Shah AB, Lahrs S, Xing Z, DeMatteo RP Endogenous granulocyte-macrophage colony-stimulating factor overexpression in vivo results in the long-term recruitment of a distinct dendritic cell population with enhanced immunostimulatory function. Lamin A/C impairments cause mitochondrial dysfunction by attenuating PGC1alpha and the NAMPT-NAD+ pathway, 2002-09-15;169(6):2875-85. 2002-09-15 [PMID: 12218100] (Mouse)
• Blumenthal RD, Reising A, Leon E Modulation of marrow proliferation and chemosensitivity by tumor-produced cytokines from syngeneic pancreatic tumor lines. Clin. Cancer Res., 2002-05-01;8(5):1301-9. 2002-05-01 [PMID: 12006552] (Mouse)

Bioinformatics

• Gene Symbol: Flt3l