Mouse IL-3 Quantikine ELISA Kit, 2 Plate Summary
Background |
The Quantikine Mouse IL-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-3 in cell culture supernates and mouse serum. It contains E. coli-expressed recombinant mouse IL-3 and antibodies raised against recombinant mouse IL-3. This Immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-3 showed dos...e curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse IL-3. |
Specificity |
Natural and recombinant mouse IL-3 |
Source |
N/A |
Assay Type |
Solid Phase Sandwich ELISA |
Inter-Assay |
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Intra-Assay |
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Spike Recovery |
|
Sample Volume |
|
Gene |
Il3 |
Applications/Dilutions
Dilutions |
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Application Notes |
No significant interference observed with available related molecules. |
Publications |
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Packaging, Storage & Formulations
Storage |
Store the unopened product at 2 - 8 degreesC. Do not use past expiration date. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Mouse IL-3 Quantikine ELISA Kit, 2 Plate
Background
Interleukin-3 (IL-3) is a secreted cytokine that stimulates the proliferation and differentiation of pluripotent hematopoietic stem cells and various lineage-committed progenitors. It also regulates the activation of mature mast cells, basophils, eosinophils, and macrophages. It is produced by activated T cells, thymic epithelial cells, activated mast cells, keratinocytes, neurons, and astrocytes. IL-3 signals through a receptor complex that contains IL-3 R alpha/CD123 and the common beta chain which is also a component of the receptors for IL-5 and GM-CSF. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils, and various myeloid leukemic cells. Human IL-3 does not show activity on mouse cells.
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Product General Protocols
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FAQs for IL-3 (M3000). (Showing 1 - 1 of 1 FAQs).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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