Mouse IL-3 Quantikine ELISA Kit, 2 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse IL-3 Quantikine ELISA Kit, 2 Plate Summary

• Background: The Quantikine Mouse IL-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-3 in cell culture supernates and mouse serum. It contains E. coli-expressed recombinant mouse IL-3 and antibodies raised against recombinant mouse IL-3. This Immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-3 showed dos...e curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse IL-3.
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• Specificity: Natural and recombinant mouse IL-3
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Il3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-3 Quantikine ELISA Kit, 2 Plate

• Hematopoietic growth factor
• IL3
• IL-3
• IL-3MGC79398
• interleukin 3 (colony-stimulating factor, multiple)
• interleukin-3
• Mast cell growth factor
• mast-cell growth factor
• MCGF
• MCGFMGC79399
• MULTI-CSF
• multilineage-colony-stimulating factor
• Multipotential colony-stimulating factor
• P-cell stimulating factor
• P-cell-stimulating factor

Background

Interleukin-3 (IL-3) is a secreted cytokine that stimulates the proliferation and differentiation of pluripotent hematopoietic stem cells and various lineage-committed progenitors. It also regulates the activation of mature mast cells, basophils, eosinophils, and macrophages. It is produced by activated T cells, thymic epithelial cells, activated mast cells, keratinocytes, neurons, and astrocytes. IL-3 signals through a receptor complex that contains IL-3 R alpha/CD123 and the common beta chain which is also a component of the receptors for IL-5 and GM-CSF. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils, and various myeloid leukemic cells. Human IL-3 does not show activity on mouse cells.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-3 (M3000)(13)

Publications using M3000

• CM Percopo, JO Krumholz, ER Fischer, LS Kraemer, M Ma, K Laky, HF Rosenberg Impact of eosinophil-peroxidase (EPX) deficiency on eosinophil structure and function in mouse airways J. Leukoc. Biol., 2018-10-04;0(0):. 2018-10-04 [PMID: 30285291] (Mouse)
• W Lu, D Jia, S An, M Mu, X Qiao, Y Liu, X Li, D Wang Calf Spleen Extractive Injection protects mice against cyclophosphamide-induced hematopoietic injury through G-CSF-mediated JAK2/STAT3 signaling Sci Rep, 2017-08-21;7(1):8402. 2017-08-21 [PMID: 28827748] (Mouse)
• Guo Y, Updegraff B, Park S, Durakoglugil D, Cruz V, Maddux S, Hwang T, O'Donnell K Comprehensive Ex Vivo Transposon Mutagenesis Identifies Genes That Promote Growth Factor Independence and Leukemogenesis. Cancer Res, 2015-12-16;76(4):773-86. 2015-12-16 [PMID: 26676752] (Mouse)
• Cunha M, Lima F, Vinolo M, Hastreiter A, Curi R, Borelli P, Fock R Protein malnutrition induces bone marrow mesenchymal stem cells commitment to adipogenic differentiation leading to hematopoietic failure. PLoS ONE, 2013-03-14;8(3):e58872. 2013-03-14 [PMID: 23516566] (Mouse)
• Kreisel D, Sugimoto S, Tietjens J, Zhu J, Yamamoto S, Krupnick AS, Carmody RJ, Gelman AE Bcl3 prevents acute inflammatory lung injury in mice by restraining emergency granulopoiesis. J. Clin. Invest., 2010-12-13;121(1):265-76. 2010-12-13 [PMID: 21157041] (Mouse)
• Ke Y, Sun D, Zhang P, Jiang G, Kaplan HJ, Shao H Suppression of established experimental autoimmune uveitis by anti-LFA-1alpha Ab. Invest. Ophthalmol. Vis. Sci., 2007-06-01;48(6):2667-75. 2007-06-01 [PMID: 17525198] (Mouse)
• Aiello FB, Keller JR, Klarmann KD, Dranoff G, Mazzucchelli R, Durum SK IL-7 induces myelopoiesis and erythropoiesis. J. Immunol., 2007-02-01;178(3):1553-63. 2007-02-01 [PMID: 17237404] (Mouse)
• Kim JM, Rasmussen JP, Rudensky AY Regulatory T cells prevent catastrophic autoimmunity throughout the lifespan of mice. Nat. Immunol., 2006-11-30;8(2):191-7. 2006-11-30 [PMID: 17136045] (Mouse)
• Liao HF, Chen YJ, Yang YC A novel polysaccharide of black soybean promotes myelopoiesis and reconstitutes bone marrow after 5-flurouracil- and irradiation-induced myelosuppression. Life Sci., 2005-02-25;77(4):400-13. 2005-02-25 [PMID: 15894009] (Mouse)
• Megid J, Kaneno R, Nozaki CN, Brito CJ, Almeida MF Increased interleukin-10 associated with low IL-6 concentration correlated with greater survival rates in mice infected by rabies virus vaccinated against it and immunomodulated with P. acnes. Comp. Immunol. Microbiol. Infect. Dis., 2004-11-01;27(6):393-411. 2004-11-01 [PMID: 15325513] (Mouse)
• Hanisch UK, Prinz M, Angstwurm K, Hausler KG, Kann O, Kettenmann H, Weber JR The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls. Eur. J. Immunol., 2001-07-01;31(7):2104-15. 2001-07-01 [PMID: 11449364] (Mouse)
• Li S, Gillessen S, Tomasson MH, Dranoff G, Gilliland DG, Van Etten RA Interleukin 3 and granulocyte-macrophage colony-stimulating factor are not required for induction of chronic myeloid leukemia-like myeloproliferative disease in mice by BCR/ABL. Blood, 2001-03-01;97(5):1442-50. 2001-03-01 [PMID: 11222392] (Mouse)
• Shih CC, Hu MC, Hu J A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells. Blood, 2000-03-15;95(6):1957-66. 2000-03-15 [PMID: 10706861] (Mouse)

FAQs for IL-3 (M3000). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Il3