Mouse IL-3 Quantikine ELISA Kit, 2 Plate

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Mouse IL-3 Quantikine ELISA Kit, 2 Plate Summary

Background
The Quantikine Mouse IL-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-3 in cell culture supernates and mouse serum. It contains E. coli-expressed recombinant mouse IL-3 and antibodies raised against recombinant mouse IL-3. This Immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-3 showed dos...e curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse IL-3.
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Specificity
Natural and recombinant mouse IL-3
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Il3

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using M3000.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-3 Quantikine ELISA Kit, 2 Plate

  • Hematopoietic growth factor
  • IL3
  • IL-3
  • IL-3MGC79398
  • interleukin 3 (colony-stimulating factor, multiple)
  • interleukin-3
  • Mast cell growth factor
  • mast-cell growth factor
  • MCGF
  • MCGFMGC79399
  • MULTI-CSF
  • multilineage-colony-stimulating factor
  • Multipotential colony-stimulating factor
  • P-cell stimulating factor
  • P-cell-stimulating factor

Background

M-CSF, also known as CSF-1, is a four-alpha-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lack cleavage and PG sites and produce an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-3 (M3000)(13)

We have publications tested in 1 confirmed species: Mouse.


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Mouse
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using M3000 Applications Species
CM Percopo, JO Krumholz, ER Fischer, LS Kraemer, M Ma, K Laky, HF Rosenberg Impact of eosinophil-peroxidase (EPX) deficiency on eosinophil structure and function in mouse airways J. Leukoc. Biol., 2018;0(0):. 2018 [PMID: 30285291] (Mouse) Mouse
W Lu, D Jia, S An, M Mu, X Qiao, Y Liu, X Li, D Wang Calf Spleen Extractive Injection protects mice against cyclophosphamide-induced hematopoietic injury through G-CSF-mediated JAK2/STAT3 signaling Sci Rep, 2017;7(1):8402. 2017 [PMID: 28827748] (Mouse) Mouse
Guo Y, Updegraff B, Park S, Durakoglugil D, Cruz V, Maddux S, Hwang T, O'Donnell K Comprehensive Ex Vivo Transposon Mutagenesis Identifies Genes That Promote Growth Factor Independence and Leukemogenesis. Cancer Res, 2015;76(4):773-86. 2015 [PMID: 26676752] (Mouse) Mouse
Cunha M, Lima F, Vinolo M, Hastreiter A, Curi R, Borelli P, Fock R Protein malnutrition induces bone marrow mesenchymal stem cells commitment to adipogenic differentiation leading to hematopoietic failure. PLoS ONE, 2013;8(3):e58872. 2013 [PMID: 23516566] (Mouse) Mouse
Kreisel D, Sugimoto S, Tietjens J, Zhu J, Yamamoto S, Krupnick AS, Carmody RJ, Gelman AE Bcl3 prevents acute inflammatory lung injury in mice by restraining emergency granulopoiesis. J. Clin. Invest., 2011;121(1):265-76. 2011 [PMID: 21157041] (Mouse) Mouse
Ke Y, Sun D, Zhang P, Jiang G, Kaplan HJ, Shao H Suppression of established experimental autoimmune uveitis by anti-LFA-1alpha Ab. Invest. Ophthalmol. Vis. Sci., 2007;48(6):2667-75. 2007 [PMID: 17525198] (Mouse) Mouse
Aiello FB, Keller JR, Klarmann KD, Dranoff G, Mazzucchelli R, Durum SK IL-7 induces myelopoiesis and erythropoiesis. J. Immunol., 2007;178(3):1553-63. 2007 [PMID: 17237404] (Mouse) Mouse
Kim JM, Rasmussen JP, Rudensky AY Regulatory T cells prevent catastrophic autoimmunity throughout the lifespan of mice. Nat. Immunol., 2007;8(2):191-7. 2007 [PMID: 17136045] (Mouse) Mouse
Liao HF, Chen YJ, Yang YC A novel polysaccharide of black soybean promotes myelopoiesis and reconstitutes bone marrow after 5-flurouracil- and irradiation-induced myelosuppression. Life Sci., 2005;77(4):400-13. 2005 [PMID: 15894009] (Mouse) Mouse
Megid J, Kaneno R, Nozaki CN, Brito CJ, Almeida MF Increased interleukin-10 associated with low IL-6 concentration correlated with greater survival rates in mice infected by rabies virus vaccinated against it and immunomodulated with P. acnes. Comp. Immunol. Microbiol. Infect. Dis., 2004;27(6):393-411. 2004 [PMID: 15325513] (Mouse) Mouse
Show All 13 Publications.

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FAQs for IL-3 (M3000). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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Bioinformatics

Gene Symbol Il3