Mouse IL-1ra/IL-1F3 Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse IL-1ra/IL-1F3 Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse IL-1ra Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IL-1ra in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-1ra and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-1ra... showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse IL-1ra.
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• Specificity: Natural and recombinant mouse IL-1ra
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Il1rn

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-1ra/IL-1F3 Quantikine ELISA Kit

• DIRA
• ICIL-1ra
• IL1F3
• IL-1F3
• IL1ra
• IL-1ra
• IL-1ra3
• IL1RN
• IL-1RN
• interleukin 1 receptor antagonist
• IRAP
• MVCD4

Background

Mouse Interleukin-1 receptor antagonist (IL-1ra) is a 22-25 kDa glycoprotein produced by a variety of cell types that antagonizes IL-1 activity (1-3). It is a member of the IL-1 family of proteins that includes IL-1 alpha and IL-1 beta . Although there is little amino acid (aa) identity (< 30%) among the three IL-1 family members, all molecules bind to the same receptors, all show a beta -trefoil structure, all are physically placed on mouse chromosome # 2, and all are believed to have evolved from a common ancestral gene (1-4). Mouse IL-1ra is synthesized as a 178 aa precursor that contains a 26 aa signal sequence plus a 152 aa mature region. There is one intrachain disulfide bond and one potential N-linked glycosylation site (3, 5, 6). Mature mouse IL-1ra shares 90%, 77%, 80%, 80%, and 78% aa sequence identity to mature rat (4), human (7), porcine (8), canine (9), and equine (10) IL-1ra, respectively. In humans, three non-secreted IL-1ra isoforms have also been identified (11-14). These result from the use of alternate start sites or exon splicing. In mice, only one of the three human intracellular isoforms has been isolated. This mouse molecule is the ortholog of the 159 aa human intracellular isoform # 1 (6, 12). The mouse intracellular form differs from the secreted precursor by only three amino acids. Cells known to secrete IL-1ra include dermal fibroblasts (15), vascular smooth muscle cells (16), intestinal columnar epithelium (17), chondrocytes (18), macrophages (19), non-keratinized oral stratified squamous epithelium (20), mast cells (21), neutrophils and monocytes (22), Sertoli cells (23), and hepatocytes (24). 

There are two type I transmembrane glycoprotein receptors for IL-1ra: the bioactive 80 kDa type I IL-1 receptor (IL-1 RI), and the inert (decoy) 65 kDa type II IL-1 receptor (IL-1 RII). IL-1ra binding to IL-1 RI competitively blocks IL-1 ( alpha or beta ) binding to the same receptor. Unlike the IL-1/IL-1 RI complex, the IL-1ra/IL-1 RI complex cannot recruit the IL-1 receptor accessory protein that is required for signal transduction. This results in receptor ligation without cell activation (1, 25). IL-1ra also competitively blocks IL-1 binding to the decoy IL-1 RII. In this case, IL-1ra may actually potentiate IL-1 activity by cancelling the functions of both antagonists. 

All activities attributed to IL-1ra are explained by its role as a competitive inhibitor of IL-1 binding to IL-1 RI (1, 2, 26, 27). In general, the ratio of IL-1ra to IL-1 beta is close to 1 in both health and disease (26, 28). To achieve total abrogation of IL-1 activity, more than 95% of all IL-1 RI receptors apparently need to be occupied by IL-1ra (29). Thus, local concentrations of secreted IL-1ra may be the critical determinants of IL-1 activity. The function of intracellular IL-1ra is less clear. While it would seem to be a simple competitor of IL-1 activity, its role may be limited to that of downmodulating non-specific inflammation associated with cell debris and death (14, 27). It has no action intracellularly; only when released through cell death would it then become a functional IL-1 antagonist.

&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-1ra/IL-1F3/IL1RN (MRA00)(31)

Publications using MRA00

• Koller, BH;Nguyen, M;Snouwaert, JN;Gabel, CA;Ting, JP; Species-specific NLRP3 regulation and its role in CNS autoinflammatory diseases Cell reports 2024-02-29 [PMID: 38427558] (Mouse)
• Fan, YC;Fong, YC;Kuo, CT;Li, CW;Chen, WY;Lin, JD;Bürtin, F;Linnebacher, M;Bui, QT;Lee, KD;Tsai, YC; Tumor-derived interleukin-1 receptor antagonist exhibits immunosuppressive functions and promotes pancreatic cancer Cell & bioscience 2023-08-10 [PMID: 37563620] (Mouse)
• CU von Linsto, SM Hindkjær, PV Nielsen, M Degn, KL Lambertsen, B Finsen, BH Clausen Bone Marrow-Derived IL-1Ra Increases TNF Levels Poststroke Cells, 2021-04-20;10(4):. 2021-04-20 [PMID: 33924148] (Mouse)
• K Tsujimaru, M Takanashi, K Sudo, A Ishikawa, S Mineo, S Ueda, K Kumagai, M Kuroda Extracellular microvesicles that originated adipose tissue derived mesenchymal stem cells have the potential ability to improve rheumatoid arthritis on mice Regenerative Therapy, 2020-12-09;15(0):305-311. 2020-12-09 [PMID: 33426233] (Mouse)
• DX Ji, LH Yamashiro, KJ Chen, N Mukaida, I Kramnik, KH Darwin, RE Vance Type I interferon-driven susceptibility to Mycobacterium tuberculosis is mediated by IL-1Ra Nat Microbiol, 2019-10-14;0(0):. 2019-10-14 [PMID: 31611644] (Mouse)
• E Lee, EL Miedzybrod, X Zhang, R Hatano, J Miyamoto, I Kimura, K Fujimoto, S Uematsu, S Rodriguez-, A Vidal-Puig, FM Gribble, F Reimann, T Miki Diet-Induced Obese Mice and Leptin-Deficient Lepob/ob Mice Exhibit Increased Circulating GIP Levels Produced by Different Mechanisms Int J Mol Sci, 2019-09-10;20(18):. 2019-09-10 [PMID: 31509948] (Mouse)
• W Wang, Y Liu, J Guo, H He, X Mi, C Chen, J Xie, S Wang, P Wu, F Cao, L Bai, Q Si, R Xiang, Y Luo miR-100 maintains phenotype of tumor-associated macrophages by targeting mTOR to promote tumor metastasis via Stat5a/IL-1ra pathway in mouse breast cancer Oncogenesis, 2018-12-19;7(12):97. 2018-12-19 [PMID: 30563983] (Mouse)
• SH Oh, C Choi, JE Noh, N Lee, YW Jeong, I Jeon, JM Shin, JH Kim, HJ Kim, JM Lee, HS Kim, OJ Kim, J Song Interleukin-1 receptor antagonist-mediated neuroprotection by umbilical cord-derived mesenchymal stromal cells following transplantation into a rodent stroke model Exp. Mol. Med., 2018-04-13;50(4):22. 2018-04-13 [PMID: 29650950] (Mouse)
• EM Nowosielsk, A Cheda, R Zdanowski, S Lewicki, BR Scott, MK Janiak Effect of internal contamination with tritiated water on the neoplastic colonies in the lungs, innate anti-tumour reactions, cytokine profile, and haematopoietic system in radioresistant and radiosensitive mice Radiat Environ Biophys, 2018-04-06;0(0):. 2018-04-06 [PMID: 29626227] (Mouse)
• BX Ross, N Gao, X Cui, TJ Standiford, J Xu, FX Yu IL-24 Promotes Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas J. Immunol, 2017-03-22;0(0):. 2017-03-22 [PMID: 28330899] (Mouse)
• C-type Lectin Mincle Recognizes Glucosyl-diacylglycerol of Streptococcus pneumoniae and Plays a Protective Role in Pneumococcal Pneumonia PLoS Pathog, 2016-12-06;12(12):e1006038. 2016-12-06 [PMID: 27923071] (Mouse)
• Controlled Release of Interleukin-1 Receptor Antagonist from Hyaluronic Acid-Chitosan Microspheres Attenuates Interleukin-1?-Induced Inflammation and Apoptosis in Chondrocytes Biomed Res Int, 2016-10-30;2016(0):6290957. 2016-10-30 [PMID: 27872853] (Rat)
• Jeschke A, Catala-Lehnen P, Sieber S, Bickert T, Schweizer M, Koehne T, Wintges K, Marshall R, Mautner A, Duchstein L, Otto B, Horst A, Amling M, Kreienkamp H, Schinke T Sharpin Controls Osteogenic Differentiation of Mesenchymal Bone Marrow Cells. J Immunol, 2015-09-11;195(8):3675-84. 2015-09-11 [PMID: 26363054] (Mouse)
• Correction: Yersinia pestis Activates Both IL-1beta and IL-1 Receptor Antagonist to Modulate Lung Inflammation during Pneumonic Plague. PLoS Pathog, 2015-05-13;11(5):e1004921. 2015-05-13 [PMID: 25970482] (Mouse)
• Carroll J, Striebel J, Race B, Phillips K, Chesebro B Prion infection of mouse brain reveals multiple new upregulated genes involved in neuroinflammation or signal transduction. J Virol, 2014-12-10;89(4):2388-404. 2014-12-10 [PMID: 25505076] (Mouse)
• Lin J, Chang Y, Yang W, Yu A, Wong C The multifaceted effects of polysaccharides isolated from Dendrobium huoshanense on immune functions with the induction of interleukin-1 receptor antagonist (IL-1ra) in monocytes. PLoS ONE, 2014-04-04;9(4):e94040. 2014-04-04 [PMID: 24705413] (Mouse)
• Izawa A, Ishihara Y, Mizutani H, Kobayashi S, Goto H, Okabe E, Takeda H, Ozawa Y, Kamiya Y, Sugita Y, Kubo K, Kamei H, Kikuchi T, Mitani A, Hayashi J, Nishihara T, Maeda H, Noguchi T Inflammatory bone loss in experimental periodontitis induced by Aggregatibacter actinomycetemcomitans in interleukin-1 receptor antagonist knockout mice. Infect Immun, 2014-02-24;82(5):1904-13. 2014-02-24 [PMID: 24566623] (Mouse)
• Wintges K, Beil F, Albers J, Jeschke A, Schweizer M, Claass B, Tiegs G, Amling M, Schinke T Impaired bone formation and increased osteoclastogenesis in mice lacking chemokine (C-C motif) ligand 5 (Ccl5). J Bone Miner Res, 2013-10-01;28(10):2070-80. 2013-10-01 [PMID: 23553711] (Mouse)
• Leifler K, Svensson S, Abrahamsson A, Bendrik C, Robertson J, Gauldie J, Olsson A, Dabrosin C Inflammation induced by MMP-9 enhances tumor regression of experimental breast cancer. J Immunol, 2013-03-15;190(8):4420-30. 2013-03-15 [PMID: 23509357] (Mouse)
• Ylostalo J, Bartosh T, Coble K, Prockop D Human mesenchymal stem/stromal cells cultured as spheroids are self-activated to produce prostaglandin E2 that directs stimulated macrophages into an anti-inflammatory phenotype. Stem Cells, 2012-10-01;30(10):2283-96. 2012-10-01 [PMID: 22865689] (Mouse)
• Lavin DN, Joesting JJ, Chiu GS, Moon ML, Meng J, Dilger RN, Freund GG Fasting induces an anti-inflammatory effect on the neuroimmune system which a high-fat diet prevents. Obesity (Silver Spring), 2011-04-28;19(8):1586-94. 2011-04-28 [PMID: 21527899] (Mouse)
• Zhao H, Leu SW, Shi L, Dedaj R, Zhao G, Garg HG, Shen L, Lien E, Fitzgerald KA, Shiedlin A, Shen H, Quinn DA, Hales CA TLR4 is a negative regulator in noninfectious lung inflammation. J. Immunol., 2010-03-31;184(9):5308-14. 2010-03-31 [PMID: 20357263] (Mouse)
• Rosenzweig HL, Martin TM, Planck SR, Galster K, Jann MM, Davey MP, Kobayashi K, Flavell RA, Rosenbaum JT Activation of NOD2 in vivo induces IL-1beta production in the eye via caspase-1 but results in ocular inflammation independently of IL-1 signaling. J. Leukoc. Biol., 2008-05-21;84(2):529-36. 2008-05-21 [PMID: 18495787] (Mouse)
• Gupta N, Su X, Popov B, Lee JW, Serikov V, Matthay MA Intrapulmonary delivery of bone marrow-derived mesenchymal stem cells improves survival and attenuates endotoxin-induced acute lung injury in mice. J. Immunol., 2007-08-01;179(3):1855-63. 2007-08-01 [PMID: 17641052] (Mouse)
• Ortiz LA, Dutreil M, Fattman C, Pandey AC, Torres G, Go K, Phinney DG Interleukin 1 receptor antagonist mediates the antiinflammatory and antifibrotic effect of mesenchymal stem cells during lung injury. Proc. Natl. Acad. Sci. U.S.A., 2007-06-14;104(26):11002-7. 2007-06-14 [PMID: 17569781] (Mouse)
• Crow AR, Song S, Semple JW, Freedman J, Lazarus AH A role for IL-1 receptor antagonist or other cytokines in the acute therapeutic effects of IVIg? Blood, 2006-09-05;109(1):155-8. 2006-09-05 [PMID: 16954498] (Mouse)
• Puehler W, Rittner HL, Mousa SA, Brack A, Krause H, Stein C, Schafer M Interleukin-1 beta contributes to the upregulation of kappa opioid receptor mrna in dorsal root ganglia in response to peripheral inflammation. Neuroscience, 2006-06-19;141(2):989-98. 2006-06-19 [PMID: 16782281] (Rat)
• Ishida Y, Kondo T, Kimura A, Matsushima K, Mukaida N Absence of IL-1 receptor antagonist impaired wound healing along with aberrant NF-kappaB activation and a reciprocal suppression of TGF-beta signal pathway. J. Immunol., 2006-05-01;176(9):5598-606. 2006-05-01 [PMID: 16622029] (Mouse)
• Blain JF, Sullivan PM, Poirier J A deficit in astroglial organization causes the impaired reactive sprouting in human apolipoprotein E4 targeted replacement mice. Neurobiol. Dis., 2005-09-19;21(3):505-14. 2005-09-19 [PMID: 16171999] (Mouse)
• Thakur A, Barrett RP, McClellan S, Hazlett LD Regulation of Pseudomonas aeruginosa corneal infection in IL-1 beta converting enzyme (ICE, caspase-1) deficient mice. Curr. Eye Res., 2004-10-01;29(4):225-33. 2004-10-01 [PMID: 15590467] (Mouse)
• Verdrengh M, Thomas JA, Hultgren OH IL-1 receptor-associated kinase 1 mediates protection against Staphylococcus aureus infection. Microbes Infect., 2004-11-01;6(14):1268-72. 2004-11-01 [PMID: 15555532] (Mouse)

Bioinformatics

• Gene Symbol: Il1rn