Mouse IL-1ra/IL-1F3 Quantikine ELISA Kit

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Mouse IL-1ra/IL-1F3 Quantikine ELISA Kit Summary

Background
The Quantikine Mouse IL-1ra Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IL-1ra in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-1ra and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-1ra... showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse IL-1ra.
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Specificity
Natural and recombinant mouse IL-1ra
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Il1rn

Applications/Dilutions

Application Notes
Interference observed with 1 or more available related molecules.
Publications
Read Publications using MRA00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-1ra/IL-1F3 Quantikine ELISA Kit

  • DIRA
  • ICIL-1ra
  • IL1F3
  • IL-1F3
  • IL1ra
  • IL-1ra
  • IL-1ra3
  • IL1RN
  • IL-1RN
  • interleukin 1 receptor antagonist
  • IRAP
  • MVCD4

Background

Mouse Interleukin-1 receptor antagonist (IL-1ra) is a 22-25 kDa glycoprotein produced by a variety of cell types that antagonizes IL-1 activity (1-3). It is a member of the IL-1 family of proteins that includes IL-1 alpha and IL-1 beta. Although there is little amino acid (aa) identity (< 30%) among the three IL-1 family members, all molecules bind to the same receptors, all show a beta -trefoil structure, all are physically placed on mouse chromosome # 2, and all are believed to have evolved from a common ancestral gene (1-4). Mouse IL-1ra is synthesized as a 178 aa precursor that contains a 26 aa signal sequence plus a 152 aa mature region. There is one intrachain disulfide bond and one potential N-linked glycosylation site (3, 5, 6). Mature mouse IL-1ra shares 90%, 77%, 80%, 80%, and 78% aa sequence identity to mature rat (4), human (7), porcine (8), canine (9), and equine (10) IL-1ra, respectively. In humans, three non-secreted IL-1ra isoforms have also been identified (11-14). These result from the use of alternate start sites or exon splicing. In mice, only one of the three human intracellular isoforms has been isolated. This mouse molecule is the ortholog of the 159 aa human intracellular isoform # 1 (6, 12). The mouse intracellular form differs from the secreted precursor by only three amino acids. Cells known to secrete IL-1ra include dermal fibroblasts (15), vascular smooth muscle cells (16), intestinal columnar epithelium (17), chondrocytes (18), macrophages (19), non-keratinized oral stratified squamous epithelium (20), mast cells (21), neutrophils and monocytes (22), Sertoli cells (23), and hepatocytes (24). 
There are two type I transmembrane glycoprotein receptors for IL-1ra: the bioactive 80 kDa type I IL-1 receptor (IL-1 RI), and the inert (decoy) 65 kDa type II IL-1 receptor (IL-1 RII). IL-1ra binding to IL-1 RI competitively blocks IL-1 ( alpha or beta ) binding to the same receptor. Unlike the IL-1/IL-1 RI complex, the IL-1ra/IL-1 RI complex cannot recruit the IL-1 receptor accessory protein that is required for signal transduction. This results in receptor ligation without cell activation (1, 25). IL-1ra also competitively blocks IL-1 binding to the decoy IL-1 RII. In this case, IL-1ra may actually potentiate IL-1 activity by cancelling the functions of both antagonists. 
All activities attributed to IL-1ra are explained by its role as a competitive inhibitor of IL-1 binding to IL-1 RI (1, 2, 26, 27). In general, the ratio of IL-1ra to IL-1 beta is close to 1 in both health and disease (26, 28). To achieve total abrogation of IL-1 activity, more than 95% of all IL-1 RI receptors apparently need to be occupied by IL-1ra (29). Thus, local concentrations of secreted IL-1ra may be the critical determinants of IL-1 activity. The function of intracellular IL-1ra is less clear. While it would seem to be a simple competitor of IL-1 activity, its role may be limited to that of downmodulating non-specific inflammation associated with cell debris and death (14, 27). It has no action intracellularly; only when released through cell death would it then become a functional IL-1 antagonist.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-1ra/IL-1F3/IL1RN (MRA00)(27)

We have publications tested in 2 confirmed species: Mouse, Rat.


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Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using MRA00 Applications Species
DX Ji, LH Yamashiro, KJ Chen, N Mukaida, I Kramnik, KH Darwin, RE Vance Type I interferon-driven susceptibility to Mycobacterium tuberculosis is mediated by IL-1Ra Nat Microbiol, 2019;0(0):. 2019 [PMID: 31611644] (Mouse) Mouse
E Lee, EL Miedzybrod, X Zhang, R Hatano, J Miyamoto, I Kimura, K Fujimoto, S Uematsu, S Rodriguez-, A Vidal-Puig, FM Gribble, F Reimann, T Miki Diet-Induced Obese Mice and Leptin-Deficient Lepob/ob Mice Exhibit Increased Circulating GIP Levels Produced by Different Mechanisms Int J Mol Sci, 2019;20(18):. 2019 [PMID: 31509948] (Mouse) Mouse
W Wang, Y Liu, J Guo, H He, X Mi, C Chen, J Xie, S Wang, P Wu, F Cao, L Bai, Q Si, R Xiang, Y Luo miR-100 maintains phenotype of tumor-associated macrophages by targeting mTOR to promote tumor metastasis via Stat5a/IL-1ra pathway in mouse breast cancer Oncogenesis, 2018;7(12):97. 2018 [PMID: 30563983] (Mouse) Mouse
SH Oh, C Choi, JE Noh, N Lee, YW Jeong, I Jeon, JM Shin, JH Kim, HJ Kim, JM Lee, HS Kim, OJ Kim, J Song Interleukin-1 receptor antagonist-mediated neuroprotection by umbilical cord-derived mesenchymal stromal cells following transplantation into a rodent stroke model Exp. Mol. Med., 2018;50(4):22. 2018 [PMID: 29650950] (Mouse) Mouse
EM Nowosielsk, A Cheda, R Zdanowski, S Lewicki, BR Scott, MK Janiak Effect of internal contamination with tritiated water on the neoplastic colonies in the lungs, innate anti-tumour reactions, cytokine profile, and haematopoietic system in radioresistant and radiosensitive mice Radiat Environ Biophys, 2018;0(0):. 2018 [PMID: 29626227] (Mouse) Mouse
BX Ross, N Gao, X Cui, TJ Standiford, J Xu, FX Yu IL-24 Promotes Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas J. Immunol, 2017;0(0):. 2017 [PMID: 28330899] (Mouse) Mouse
C-type Lectin Mincle Recognizes Glucosyl-diacylglycerol of Streptococcus pneumoniae and Plays a Protective Role in Pneumococcal Pneumonia PLoS Pathog, 2016;12(12):e1006038. 2016 [PMID: 27923071] (Mouse) Mouse
Controlled Release of Interleukin-1 Receptor Antagonist from Hyaluronic Acid-Chitosan Microspheres Attenuates Interleukin-1?-Induced Inflammation and Apoptosis in Chondrocytes Biomed Res Int, 2016;2016(0):6290957. 2016 [PMID: 27872853]
Jeschke A, Catala-Lehnen P, Sieber S, Bickert T, Schweizer M, Koehne T, Wintges K, Marshall R, Mautner A, Duchstein L, Otto B, Horst A, Amling M, Kreienkamp H, Schinke T Sharpin Controls Osteogenic Differentiation of Mesenchymal Bone Marrow Cells. J Immunol, 2015;195(8):3675-84. 2015 [PMID: 26363054] (Mouse) Mouse
Correction: Yersinia pestis Activates Both IL-1beta and IL-1 Receptor Antagonist to Modulate Lung Inflammation during Pneumonic Plague. PLoS Pathog, 2015;11(5):e1004921. 2015 [PMID: 25970482] (Mouse) Mouse
Show All 27 Publications.

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FAQs for IL-1ra/IL-1F3/IL1RN (MRA00). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP MRA00

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Bioinformatics

Gene Symbol Il1rn