Mouse IL-1ra/IL-1F3 DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse IL-1ra/IL-1F3 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Il1rn

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-1ra/IL-1F3 DuoSet ELISA, 15 Plate

• DIRA
• ICIL-1ra
• IL1F3
• IL-1F3
• IL1ra
• IL-1ra
• IL-1ra3
• IL1RN
• IL-1RN
• interleukin 1 receptor antagonist
• IRAP
• MVCD4

Background

Mouse Interleukin-1 receptor antagonist (IL-1ra) is a 22-25 kDa glycoprotein produced by a variety of cell types that antagonizes IL-1 activity (1-3). It is a member of the IL-1 family of proteins that includes IL-1 alpha and IL-1 beta . Although there is little amino acid (aa) identity (< 30%) among the three IL-1 family members, all molecules bind to the same receptors, all show a beta -trefoil structure, all are physically placed on mouse chromosome # 2, and all are believed to have evolved from a common ancestral gene (1-4). Mouse IL-1ra is synthesized as a 178 aa precursor that contains a 26 aa signal sequence plus a 152 aa mature region. There is one intrachain disulfide bond and one potential N-linked glycosylation site (3, 5, 6). Mature mouse IL-1ra shares 90%, 77%, 80%, 80%, and 78% aa sequence identity to mature rat (4), human (7), porcine (8), canine (9), and equine (10) IL-1ra, respectively. In humans, three non-secreted IL-1ra isoforms have also been identified (11-14). These result from the use of alternate start sites or exon splicing. In mice, only one of the three human intracellular isoforms has been isolated. This mouse molecule is the ortholog of the 159 aa human intracellular isoform # 1 (6, 12). The mouse intracellular form differs from the secreted precursor by only three amino acids. Cells known to secrete IL-1ra include dermal fibroblasts (15), vascular smooth muscle cells (16), intestinal columnar epithelium (17), chondrocytes (18), macrophages (19), non-keratinized oral stratified squamous epithelium (20), mast cells (21), neutrophils and monocytes (22), Sertoli cells (23), and hepatocytes (24). 

There are two type I transmembrane glycoprotein receptors for IL-1ra: the bioactive 80 kDa type I IL-1 receptor (IL-1 RI), and the inert (decoy) 65 kDa type II IL-1 receptor (IL-1 RII). IL-1ra binding to IL-1 RI competitively blocks IL-1 ( alpha or beta ) binding to the same receptor. Unlike the IL-1/IL-1 RI complex, the IL-1ra/IL-1 RI complex cannot recruit the IL-1 receptor accessory protein that is required for signal transduction. This results in receptor ligation without cell activation (1, 25). IL-1ra also competitively blocks IL-1 binding to the decoy IL-1 RII. In this case, IL-1ra may actually potentiate IL-1 activity by cancelling the functions of both antagonists. 

All activities attributed to IL-1ra are explained by its role as a competitive inhibitor of IL-1 binding to IL-1 RI (1, 2, 26, 27). In general, the ratio of IL-1ra to IL-1 beta is close to 1 in both health and disease (26, 28). To achieve total abrogation of IL-1 activity, more than 95% of all IL-1 RI receptors apparently need to be occupied by IL-1ra (29). Thus, local concentrations of secreted IL-1ra may be the critical determinants of IL-1 activity. The function of intracellular IL-1ra is less clear. While it would seem to be a simple competitor of IL-1 activity, its role may be limited to that of downmodulating non-specific inflammation associated with cell debris and death (14, 27). It has no action intracellularly; only when released through cell death would it then become a functional IL-1 antagonist.

Publications for IL-1ra/IL-1F3/IL1RN (DY480)(20)

Publications using DY480

• Ely, EV;Kapinski, AT;Paradi, SG;Tang, R;Guilak, F;Collins, KH; Designer Fat Cells: Adipogenic Differentiation of CRISPR-Cas9 Genome-Engineered Induced Pluripotent Stem Cells bioRxiv : the preprint server for biology 2023-10-26 [PMID: 37961399] (Mouse)
• Griffith, JW;Faustino, LD;Cottrell, VI;Nepal, K;Hariri, LP;Chiu, RS;Jones, MC;Julé, A;Gabay, C;Luster, AD; Regulatory T cell-derived IL-1Ra suppresses the innate response to respiratory viral infection Nature immunology 2023-11-09 [PMID: 37945820] (Mouse)
• Schädel, P;Wichmann-Costaganna, M;Czapka, A;Gebert, N;Ori, A;Werz, O; Short-Term Caloric Restriction and Subsequent Re-Feeding Compromise Liver Health and Associated Lipid Mediator Signaling in Aged Mice Nutrients 2023-08-21 [PMID: 37630850] (Mouse)
• P Schädel, A Czapka, N Gebert, ID Jacobsen, A Ori, O Werz Metabololipidomic and proteomic profiling reveals aberrant macrophage activation and interrelated immunomodulatory mediator release during aging Aging Cell, 2023-04-26;0(0):e13856. 2023-04-26 [PMID: 37101405] (Mouse)
• L Pferdehirt, AR Damato, M Dudek, QJ Meng, ED Herzog, F Guilak Synthetic gene circuits for preventing disruption of the circadian clock due to interleukin-1-induced inflammation Science Advances, 2022-05-25;8(21):eabj8892. 2022-05-25 [PMID: 35613259] (Mouse)
• P Rozier, M Maumus, ATJ Maria, K Toupet, C Jorgensen, P Guilpain, D Noël Lung Fibrosis Is Improved by Extracellular Vesicles from IFNgamma-Primed Mesenchymal Stromal Cells in Murine Systemic Sclerosis Cells, 2021-10-13;10(10):. 2021-10-13 [PMID: 34685707] (Mouse)
• CM Percopo, M McCullough, AR Limkar, KM Druey, HF Rosenberg Impact of controlled high-sucrose and high-fat diets on eosinophil recruitment and cytokine content in allergen-challenged mice PLoS ONE, 2021-08-12;16(8):e0255997. 2021-08-12 [PMID: 34383839] (Mouse)
• XAMH van Dieren, T Sancerni, MC Alves-Guer, R Stienstra The role of uncoupling protein 2 in macrophages and its impact on obesity-induced adipose tissue inflammation and insulin resistance J Biol Chem, 2020-01-01;0(0):. 2020-01-01 [PMID: 33051201] (Mouse)
• Z Julier, R Karami, B Nayer, YZ Lu, AJ Park, K Maruyama, GA Kuhn, R Müller, S Akira, MM Martino Enhancing the regenerative effectiveness of growth factors by local inhibition of interleukin-1 receptor signaling Sci Adv, 2020;6(24):eaba7602. 2020 [PMID: 32582857] (Mouse)
• Z Julier, R Karami, B Nayer, YZ Lu, AJ Park, K Maruyama, GA Kuhn, R Müller, S Akira, MM Martino Enhancing the regenerative effectiveness of growth factors by local inhibition of interleukin-1 receptor signaling Sci Adv, 2020-06-12;6(24):eaba7602. 2020-06-12 [PMID: 32582857] (Mouse)
• V Besnard, R Dagher, T Madjer, A Joannes, M Jaillet, M Kolb, P Bonniaud, LA Murray, MA Sleeman, B Crestani Identification of periplakin as a major regulator of lung injury and repair in mice JCI Insight, 2018-03-08;3(5):. 2018-03-08 [PMID: 29515024] (Mouse)
• JX Yang, KC Hsieh, YL Chen, CK Lee, M Conti, TH Chuang, CP Wu, SC Jin Phosphodiesterase 4B negatively regulates endotoxin-activated interleukin-1 receptor antagonist responses in macrophages Sci Rep, 2017-04-06;7(0):46165. 2017-04-06 [PMID: 28383060] (Mouse)
• Cell autonomous or systemic EGFR blockade alters the immune-environment in squamous cell carcinomas Int J Cancer, 2016-08-29;0(0):. 2016-08-29 [PMID: 27509256] (Mouse)
• Avila L, Bruggemann T, Bobinski F, da Silva M, Oliveira R, Martins D, Mazzardo-Martins L, Duarte M, de Souza L, Dafre A, Vieira R, Santos A, Bonorino K, Hizume Kunzler D Effects of High-Intensity Swimming on Lung Inflammation and Oxidative Stress in a Murine Model of DEP-Induced Injury. PLoS ONE, 2015-09-02;10(9):e0137273. 2015-09-02 [PMID: 26332044] (Mouse)
• Hoyt T, Dobrinen E, Kochetkova I, Meissner N B cells modulate systemic responses to Pneumocystis murina lung infection and protect on-demand hematopoiesis via T cell-independent innate mechanisms when type I interferon signaling is absent. Infect Immun, 2014-12-01;83(2):743-58. 2014-12-01 [PMID: 25452554] (Mouse)
• Stahl M, Schupp J, Jager B, Schmid M, Zissel G, Muller-Quernheim J, Prasse A Lung collagens perpetuate pulmonary fibrosis via CD204 and M2 macrophage activation. PLoS ONE, 2013-11-20;8(11):e81382. 2013-11-20 [PMID: 24278429] (Human)
• Horton J, Hudak K, Chung E, White A, Scroggins B, Burkeen J, Citrin D Mesenchymal stem cells inhibit cutaneous radiation-induced fibrosis by suppressing chronic inflammation. Stem Cells, 2013-10-01;31(10):2231-41. 2013-10-01 [PMID: 23897677] (Mouse)
• Lipocalin 2 deficiency dysregulates iron homeostasis and exacerbates endotoxin-induced sepsis. J. Immunol., 2012-07-11;189(4):1911-9. 2012-07-11 [PMID: 22786765] (Mouse)
• Barber DL, Mayer-Barber KD, Feng CG CD4 T cells promote rather than control tuberculosis in the absence of PD-1-mediated inhibition. J. Immunol., 2010-12-20;186(3):1598-607. 2010-12-20 [PMID: 21172867] (Mouse)
• Lamacchia C, Palmer G, Bischoff L, Rodriguez E, Talabot-Ayer D, Gabay C Distinct roles of hepatocyte- and myeloid cell-derived IL-1 receptor antagonist during endotoxemia and sterile inflammation in mice. J. Immunol., 2010-07-16;185(0):2516-24. 2010-07-16 [PMID: 20639493] (Mouse)

FAQs for IL-1ra/IL-1F3/IL1RN (DY480). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: Il1rn