Mouse IFN-beta DuoSet ELISA, 5 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse IFN-beta DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IFN-beta DuoSet ELISA, 5 Plate

• Fibroblast interferon
• IFB
• IFBIFNB
• IFF
• IFNB
• IFNB1
• IFNbeta
• IFN-beta
• interferon beta
• interferon, beta 1, fibroblast
• MGC96956

Background

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Publications for IFN-beta (DY8234-05)(56)

Publications using DY8234-05

• Han, Z;Xiong, S;Zeng, K;Xiao, Z;Zhang, L;Zhang, Y;Guo, J;Peng, W;Xie, Y;Liu, W;Yu, X; Palmitoylation of TBK1 enhances the type I interferon signaling and strengthens anti-malarial immunity in mice Nature communications 2025-11-18 [PMID: 41253837] (Mouse)
• Speaks, S;Papa, J;McFadden, M;Roettger, JE;Liu, BD;Mohan, S;Reznik, BM;Leumi, S;Cable, JM;Forero, A;Yount, JS; Gasdermin E is Dispensable for H1N1 Influenza Virus Pathogenesis in Mice bioRxiv : the preprint server for biology 2025-07-29 [PMID: 40766393] (Mouse)
• Matsuo-Dapaah, J;Alshaweesh, J;Lee, MSJ;Hayashi, T;Dash, R;Kuroda, M;Tainaka, K;Ozawa, M;Kuratani, A;Yamamoto, M;Liu, K;Fukui, R;Miyake, K;Kobiyama, K;Rénia, L;Ishii, KJ;Coban, C; IFN?-inducible Gbp4 and Irgb6 contribute to experimental cerebral malaria pathology in the olfactory bulb mBio 2025-07-03 [PMID: 40607809] (Transgenic Mouse)
• Sharma, L;Singh, RB;Ngeow, C;van der Geest, R;Duray, AM;Tolman, NJ;McVerry, BJ;Dela Cruz, CS;Alcorn, JF;Bain, W;Robinson, KM; Therapeutic JAK inhibition does not impact lung injury during viral or bacterial pneumonia in male mice Physiological reports 2025-02-01 [PMID: 39921246] (Mouse)
• Watanabe, T;Hayashi, M;Arai, M;Matsushita, S;Handa, H;Kawano, M; Interferon-? production in response to the cytotoxic T lymphocyte epitope within an antigen incorporated in simian virus 40 virus-like particles Heliyon 2025-01-30 [PMID: 39897799] (Mouse)
• Drobek, A;Bernaleau, L;Delacrétaz, M;Calderon Copete, S;Royer-Chardon, C;Longepierre, M;Monguió-Tortajada, M;Korzeniowski, J;Rotman, S;Marquis, J;Rebsamen, M; The TLR7/9 adaptors TASL and TASL2 mediate IRF5-dependent antiviral responses and autoimmunity in mouse Nature communications 2025-01-24 [PMID: 39856058] (Mouse, Transgenic Mouse)
• Chan, FHM;Yeap, HW;Liu, Z;Rosli, SN;Low, KE;Bonne, I;Wu, Y;Chong, SZ;Chen, KW; Plasticity of cell death pathways ensures GSDMD activation during Yersinia pseudotuberculosis infection Cell reports 2025-01-16 [PMID: 39823227] (Mouse)
• Wu, Y;Park, J;Le, Q;Byun, J;Choi, J;Xu, H;Lee, J;Oh, Y; NET formation-mediated in situ protein delivery to the inflamed central nervous system Nature communications 2024-12-30 [PMID: 39737919] (N/A)
• Olawuyi, IF;Heo, E;Jeong, M;Kim, JH;Park, JJ;Chae, J;Gwon, S;Do Lee, S;Kim, H;Ojulari, OV;Song, YB;Lee, BH;Gu, BB;Kim, SR;Lee, JH;Lee, W;Hwang, JS;Nam, JO;Hahn, D;Byun, S; Acidic polysaccharide from the edible insect Protaetia brevitarsis seulensis activates antiviral immunity to suppress norovirus infection Carbohydrate polymers 2025-01-01 [PMID: 39486915] (Mouse)
• Denz, PJ;Speaks, S;Kenney, AD;Eddy, AC;Papa, JL;Roettger, J;Scace, SC;Rubrum, A;Hemann, EA;Forero, A;Webby, RJ;Bowman, AS;Yount, JS; Innate immune control of influenza virus interspecies adaptation via IFITM3 Nature communications 2024-10-30 [PMID: 39477971] (Mouse, Transgenic Mouse)
• An, S;Oh, J;Shon, HJ;Song, J;Choi, YS;Kim, D; Co-adjuvanting Nod2-stimulating bacteria with a TLR7 agonist elicits potent protective immunity against respiratory virus infection International journal of antimicrobial agents 2024-10-28 [PMID: 39477030] (Mouse)
• Hong, NE;Chaplin, A;Di, L;Ravodina, A;Bevan, GH;Gao, H;Asase, C;Gangwar, RS;Cameron, MJ;Mignery, M;Cherepanova, O;Finn, AV;Nayak, L;Pieper, AA;Maiseyeu, A; Nanoparticle-based itaconate treatment recapitulates low-cholesterol/low-fat diet-induced atherosclerotic plaque resolution Cell reports 2024-10-27 [PMID: 39466775] (Mouse)
• Ainslie, K; Modifying Post-Surgical Immunity: Controlled Release of TLR7/8 Agonist for Immune Mediated Clearance of Glioblastoma Research square 2024-09-26 [PMID: 39399681] (Mouse)
• Taheri, M;Tehrani, HA;Farzad, SA;Korourian, A;Arefian, E;Ramezani, M; The potential of mesenchymal stem cell coexpressing cytosine deaminase and secretory IL18-FC chimeric cytokine in suppressing glioblastoma recurrence International immunopharmacology 2024-09-04 [PMID: 39236459] (Mouse)
• Jensen, SB;Jæhger, DE;Serrano-Chávez, E;Halldórsdóttir, HR;Engel, TB;Jørgensen, JS;Björgvinsdóttir, UJ;Kostrikov, S;Scheeper, MJ;Ringgaard, L;Bruun, LM;Stavnsbjerg, C;Christensen, E;Bak, M;Thuroczy, J;Balogh, L;Jensen, ATI;Melander, F;Kjaer, A;Henriksen, JR;Hansen, AE;Andresen, TL; An in situ depot for the sustained release of a TLR7/8 agonist in combination with a TGF? inhibitor promotes anti-tumor immune responses Nature communications 2024-09-03 [PMID: 39227589] (Mouse, Transgenic Mouse)
• Honwad, HH;Najibi, M;Koscso, B;Bogunovic, M;Irazoqui, JE; TFEB-Mediated Pro-inflammatory Response in Murine Macrophages Induced by Acute Alpha7 Nicotinic Receptor Activation bioRxiv : the preprint server for biology 2024-08-10 [PMID: 39211236] (Mouse)
• Gabele, L;Bochow, I;Rieke, N;Sieben, C;Michaelsen-Preusse, K;Hosseini, S;Korte, M; H7N7 viral infection elicits pronounced, sex-specific neuroinflammatory responses in vitro Frontiers in cellular neuroscience 2024-08-07 [PMID: 39171200] (Mouse)
• Huang, Q;Yang, G;Tang, C;Dou, B;Hu, Y;Liu, H;Wu, X;Zhang, H;Wang, H;Xu, L;Yang, XD;Xu, Y;Zheng, Y; Rujin Jiedu decoction protects against influenza virus infection by modulating gut microbiota Heliyon 2024-07-15 [PMID: 39071618] (Mouse)
• Li, J;Moresco, P;Fearon, DT; Intratumoral NKT cell accumulation promotes antitumor immunity in pancreatic cancer Proceedings of the National Academy of Sciences of the United States of America 2024-07-16 [PMID: 38980903] (Mouse)
• Miles, MA;Luong, R;To, EE;Erlich, JR;Liong, S;Liong, F;Logan, JM;O'Leary, J;Brooks, DA;Selemidis, S; TLR9 Monotherapy in Immune-Competent Mice Suppresses Orthotopic Prostate Tumor Development Cells 2024-01-02 [PMID: 38201300] (Mouse)
• Zhang, H;Xue, K;Li, W;Yang, X;Gou, Y;Su, X;Qian, F;Sun, L; Cullin5 drives experimental asthma exacerbations by modulating alveolar macrophage antiviral immunity Nature communications 2024-01-04 [PMID: 38177117] (Mouse, Transgenic Mouse)
• Heinzl, GC;Eriksen, DB;Johnsen, PR;Scarafoni, A;Frøkiær, H; Protein Concentration Affects the Food Allergen ?-Conglutin Uptake and Bacteria-Induced Cytokine Production in Dendritic Cells Biomolecules 2023-10-16 [PMID: 37892213] (Mouse)
• Wang, X;Wang, Y;Cao, A;Luo, Q;Chen, D;Zhao, W;Xu, J;Li, Q;Bu, X;Quan, J; Development of cyclopeptide inhibitors of cGAS targeting protein-DNA interaction and phase separation Nature communications 2023-10-02 [PMID: 37783727] (Mouse)
• Barasa, L;Chaudhuri, S;Zhou, JY;Jiang, Z;Choudhary, S;Green, RM;Wiggin, E;Cameron, M;Humphries, F;Fitzgerald, KA;Thompson, PR; Development of LB244, an Irreversible STING Antagonist Journal of the American Chemical Society 2023-09-20 [PMID: 37695732] (Mouse)
• Rasmussen, M;Alvik, K;Kannen, V;Olafsen, NE;Erlingsson, LAM;Grimaldi, G;Takaoka, A;Grant, DM;Matthews, J; Loss of PARP7 Increases Type I Interferon Signaling in EO771 Breast Cancer Cells and Prevents Mammary Tumor Growth by Increasing Antitumor Immunity Cancers 2023-07-20 [PMID: 37509350] (Mouse)
• Pérez-Cabello, JA;Silvera-Carrasco, L;Franco, JM;Capilla-González, V;Armaos, A;Gómez-Lima, M;García-García, R;Yap, XW;Leal-Lasarte, M;Lall, D;Baloh, RH;Martínez, S;Miyata, Y;Tartaglia, GG;Sawarkar, R;García-Domínguez, M;Pozo, D;Roodveldt, C; MAPK/MAK/MRK overlapping kinase (MOK) controls microglial inflammatory/type-I IFN responses via Brd4 and is involved in ALS Proceedings of the National Academy of Sciences of the United States of America 2023-07-11 [PMID: 37399380] (Mouse)
• Hu, Z;Xie, Y;Lu, J;Yang, J;Zhang, J;Jiang, H;Li, H;Zhang, Y;Wu, D;Zeng, K;Bai, X;Yu, X; VANGL2 inhibits antiviral IFN-I signaling by targeting TBK1 for autophagic degradation Science advances 2023-06-23 [PMID: 37352355] (Transgenic Mouse)
• Weilun Pang, Fenghua Hu C9ORF72 suppresses JAK-STAT mediated inflammation iScience 2023-05-19 [PMID: 37250330] (Mouse)
• Jack P. Green, Lina Y. El-Sharkawy, Stefan Roth, Jie Zhu, Jiayu Cao, Andrew G. Leach et al. Discovery of an inhibitor of DNA-driven inflammation that preferentially targets the AIM2 inflammasome iScience 2023-05-19 [PMID: 37216118] (Mouse)
• D Levy, SN Abadchi, N Shababi, MR Ravari, NH Pirolli, C Bergeron, A Obiorah, F Mokhtari-E, S Gheshlaghi, JM Abraham, IM Smith, E Powsner, T Solomon, JW Harmon, SM Jay Induced pluripotent stem cell-derived extracellular vesicles promote wound repair in a diabetic mouse model via an anti-inflammatory immunomodulatory mechanism bioRxiv : the preprint server for biology, 2023-03-23;0(0):. 2023-03-23 [PMID: 36993554] (Mouse)
• R Falahat, A Berglund, P Perez-Vill, RM Putney, I Hamaidi, S Kim, S Pilon-Thom, GN Barber, JJ Mulé Epigenetic state determines the in vivo efficacy of STING agonist therapy Nature Communications, 2023-03-22;14(1):1573. 2023-03-22 [PMID: 36949064] (Mouse)
• S Speaks, A Zani, A Solstad, A Kenney, L Zhang, AC Eddy, AO Amer, R Robinson, C Cai, J Ma, EA Hemann, A Forero, JS Yount Loss of Gasdermin D is protective against influenza virus-induced inflammation and mortality bioRxiv : the preprint server for biology, 2023-04-07;0(0):. 2023-04-07 [PMID: 36945485] (Mouse)
• A Hooftman, CG Peace, DG Ryan, EA Day, M Yang, AF McGettrick, M Yin, EN Montano, L Huo, JE Toller-Kaw, V Zecchini, TAJ Ryan, A Bolado-Car, AM Casey, HA Prag, ASH Costa, G De Los San, M Ishimori, DJ Wallace, S Venuturupa, E Nikitopoul, N Frizzell, C Johansson, A Von Kriegs, MP Murphy, C Jefferies, C Frezza, LAJ O'Neill Macrophage fumarate hydratase restrains mtRNA-mediated interferon production Nature, 2023-03-08;615(7952):490-498. 2023-03-08 [PMID: 36890227] (Mouse, Transgenic Mouse)
• Y Wang, Y Wang, L Ding, X Ren, B Wang, L Wang, S Zhao, X Yue, Z Wu, C Li, X Liang, C Ma, L Gao Tim-4 reprograms cholesterol metabolism to suppress antiviral innate immunity by disturbing the Insig1-SCAP interaction in macrophages Cell Reports, 2022-11-29;41(9):111738. 2022-11-29 [PMID: 36450259] (Mouse, Transgenic Mouse)
• L Willemsen, KHM Prange, AE Neele, CPAA van Roomen, M Gijbels, GR Griffith, MD Toom, L Beckers, R Siebeler, NJ Spann, HJ Chen, LA Bosmans, A Gorbatenko, S van Wouw, N Zelcer, H Jacobs, F van Leeuwe, MPJ de Winther DOT1L regulates lipid biosynthesis and inflammatory responses in macrophages and promotes atherosclerotic plaque stability Cell Reports, 2022-11-22;41(8):111703. 2022-11-22 [PMID: 36417856] (Transgenic Mouse)
• N Bartonicek, R Rouet, J Warren, C Loetsch, GS Rodriguez, S Walters, F Lin, D Zahra, J Blackburn, JM Hammond, ALM Reis, IW Deveson, N Zammit, M Zeraati, S Grey, D Christ, JS Mattick, T Chtanova, R Brink, ME Dinger, RJ Weatheritt, J Sprent, C King The retroelement Lx9 puts a brake on the immune response to virus infection Nature, 2022-08-10;0(0):. 2022-08-10 [PMID: 35948641] (Mouse)
• Z Qin, X Fang, W Sun, Z Ma, T Dai, S Wang, Z Zong, H Huang, H Ru, H Lu, B Yang, S Lin, F Zhou, L Zhang Deactylation by SIRT1 enables liquid-liquid phase separation of IRF3/IRF7 in innate antiviral immunity Nature Immunology, 2022-07-25;0(0):. 2022-07-25 [PMID: 35879450] (Mouse)
• M Perego, S Fu, Y Cao, A Kossenkov, M Yao, K Alicea-Tor, W Liu, Z Jiang, Z Chen, SY Fuchs, J Zhou, DI Gabrilovic Mechanisms regulating transitory suppressive activity of neutrophils in newborns: PMNs-MDSCs in newborns Oncogene, 2022-06-21;0(0):. 2022-06-21 [PMID: 35726818] (Mouse)
• CJ Batty, MD Gallovic, J Williams, TM Ross, EM Bachelder, KM Ainslie Multiplexed electrospray enables high throughput production of cGAMP microparticles to serve as an adjuvant for a broadly acting influenza vaccine International journal of pharmaceutics, 2022-05-24;622(0):121839. 2022-05-24 [PMID: 35623484] (Mouse)
• AD Kenney, SL Aron, C Gilbert, N Kumar, P Chen, A Eddy, L Zhang, A Zani, N Vargas-Mal, S Speaks, J Kawahara, PJ Denz, L Dorn, F Accornero, J Ma, H Zhu, MVS Rajaram, C Cai, RA Langlois, JS Yount Influenza virus replication in cardiomyocytes drives heart dysfunction and fibrosis Science Advances, 2022-05-11;8(19):eabm5371. 2022-05-11 [PMID: 35544568] (Mouse)
• JT Hinkle, J Patel, N Panicker, SS Karuppagou, D Biswas, B Belingon, R Chen, S Brahmachar, O Pletnikova, JC Troncoso, VL Dawson, TM Dawson STING mediates neurodegeneration and neuroinflammation in nigrostriatal alpha-synucleinopathy Proceedings of the National Academy of Sciences of the United States of America, 2022-04-08;119(15):e2118819119. 2022-04-08 [PMID: 35394877] (Mouse)
• D Olmeda, D Cerezo-Wal, C Mucientes, TG Calvo, E Cañón, D Alonso-Cur, N Ibarz, J Muñoz, JL Rodriguez-, P Ortiz-Rome, S Ortega, MS Soengas Live imaging of neolymphangiogenesis identifies acute antimetastatic roles of dsRNA mimics Embo Molecular Medicine, 2021-11-11;0(0):e12924. 2021-11-11 [PMID: 34762341] (Mouse)
• DE Jæhger, ML Hübbe, MK Kræmer, G Clergeaud, AV Olsen, C Stavnsbjer, MN Wiinholt, A Kjær, JR Henriksen, AE Hansen, TL Andresen Enhancing adoptive CD8 T cell therapy by systemic delivery of tumor associated antigens Scientific Reports, 2021-10-05;11(1):19794. 2021-10-05 [PMID: 34611284] (Mouse)
• S Rastogi, S Ellinwood, J Augenstrei, KD Mayer-Barb, V Briken Mycobacterium tuberculosis inhibits the NLRP3 inflammasome activation via its phosphokinase PknF PloS Pathogens, 2021-07-29;17(7):e1009712. 2021-07-29 [PMID: 34324582] (Mouse)
• Y Li, C Bax, J Patel, T Vazquez, A Ravishanka, MM Bashir, M Grinnell, D Diaz, VP Werth Plasma-derived DNA containing-extracellular vesicles induce STING-mediated proinflammatory responses in dermatomyositis Theranostics, 2021-05-21;11(15):7144-7158. 2021-05-21 [PMID: 34158841] (Mouse)
• J Chen, M Qiu, Z Ye, T Nyalile, Y Li, Z Glass, X Zhao, L Yang, J Chen, Q Xu In situ cancer vaccination using lipidoid nanoparticles Science Advances, 2021-05-05;7(19):. 2021-05-05 [PMID: 33952519] (Mouse)
• M Najibi, HH Honwad, JA Moreau, SM Becker, JE Irazoqui A NOVEL NOX/PHOX-CD38-NAADP-TFEB AXIS IMPORTANT FOR MACROPHAGE ACTIVATION DURING BACTERIAL PHAGOCYTOSIS Autophagy, 2021-04-13;0(0):. 2021-04-13 [PMID: 33818279] (Mouse)
• N Hijaze, M Ledersnaid, E Simanovich, S Kassem, MA Rahat Inducing regulated necrosis and shifting macrophage polarization with anti-EMMPRIN antibody (161-pAb) and complement factors J Leukoc Biol, 2020-11-17;0(0):. 2020-11-17 [PMID: 33205451] (Mouse)
• JC DuBois, AK Ray, P Davies, B Shafit-Zag Anti-Axl antibody treatment reduces the severity of experimental autoimmune encephalomyelitis J Neuroinflammation, 2020-10-29;17(1):324. 2020-10-29 [PMID: 33121494] (Mouse)
• M Sermershei, AD Kenney, PH Lin, TM McMichael, C Cai, K Gumpper, TMA Adesanya, H Li, X Zhou, KH Park, JS Yount, J Ma MG53 suppresses interferon-&beta and inflammation via regulation of ryanodine receptor-mediated intracellular calcium signaling Nat Commun, 2020-07-17;11(1):3624. 2020-07-17 [PMID: 32681036] (Human)
• J Wu, N Weisshaar, A Hotz-Wagen, A Madi, S Ma, A Mieg, M Hering, K Mohr, T Schlimbach, H Borgers, G Cui Skeletal muscle antagonizes antiviral CD8+ T cell exhaustion Sci Adv, 2020-06-12;6(24):eaba3458. 2020-06-12 [PMID: 32582853] (Mouse)
• O Danov, L Lasswitz, H Obernolte, C Hesse, A Braun, S Wronski, K Sewald Rupintrivir reduces RV-induced TH-2 cytokine IL-4 in precision-cut lung slices (PCLS) of HDM-sensitized mice ex vivo Respir. Res., 2019-10-22;20(1):228. 2019-10-22 [PMID: 31640701] (Mouse)
• L Yue, M Yan, ML Tremblay, TJ Lin, H Li, T Yang, X Song, T Xie, Z Xie PTP1B negatively regulates nitric oxide-mediated Pseudomonas aeruginosa killing by neutrophils PLoS ONE, 2019-09-18;14(9):e0222753. 2019-09-18 [PMID: 31532798] (Mouse)
• M Coorens, VAF Schneider, AM de Groot, A van Dijk, M Meijerink, JM Wells, MR Scheenstra, EJA Veldhuizen, HP Haagsman Cathelicidins Inhibit Escherichia coli-Induced TLR2 and TLR4 Activation in a Viability-Dependent Manner J. Immunol., 2017-07-14;0(0):. 2017-07-14 [PMID: 28710255] (Mouse)
• PC Campos, MT Gomes, ES Guimarães, G Guimarães, SC Oliveira TLR7 and TLR3 Sense Brucella abortus RNA to Induce Proinflammatory Cytokine Production but They Are Dispensable for Host Control of Infection Front Immunol, 2017-01-23;8(0):28. 2017-01-23 [PMID: 28167945] (Mouse)
• Joerg Mattes TRAIL signaling is pro-inflammatory and pro-viral in a murine model of rhinovirus 1B infection Am. J. Physiol. Lung Cell Mol. Physiol., 2016-11-11;0(0):ajplung.00200. 2016-11-11 [PMID: 27836899] (Mouse)

FAQs for IFN-beta (DY8234-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.