Mouse Flt-3 Ligand/FLT3L DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse Flt-3 Ligand/FLT3L DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Flt3l

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Flt-3 Ligand/FLT3L DuoSet ELISA, 15 Plate

• FL
• FLG3L
• Flt3 ligand
• Flt-3 Ligand
• Flt3L
• FLT3LG
• fms-related tyrosine kinase 3 ligand
• SL cytokine

Background

Flt-3 Ligand, also known as FL, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Mature human Flt-3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. Within the ECD, human Flt-3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt-3 Ligand, respectively. Human and mouse Flt-3 Ligand show cross-species activity. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates. Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain. Alternate splicing of human Flt-3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine domain. Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation. Animal studies also show Flt-3 Ligand to reduce the severity of experimentally induced allergic inflammation.

Publications for Flt-3 Ligand/FLT3L (DY427)(6)

Publications using DY427

• Chun, D;Park, J;Lee, S;Kim, HJ;Park, JE;Kang, SJ; Flt3L enhances clonal diversification and selective expansion of intratumoral CD8+ T cells while differentiating into effector-like cells Cell reports 2024-11-30 [PMID: 39616612] (Human, Mouse, Transgenic Mouse, Xenograft)
• Yu, CI;Maser, R;Marches, F;Banchereau, J;Palucka, K; Long-term engraftment of adult hematopoietic progenitors in a novel model of humanized mice bioRxiv : the preprint server for biology 2023-10-03 [PMID: 37873457] (Mouse)
• MB Lutz, S Ali, C Audiger, SE Autenrieth, L Berod, V Bigley, L Cyran, M Dalod, J Dörrie, D Dudziak, G Flórez-Gra, L Giusiano, GJ Godoy, M Heuer, AB Krug, CHK Lehmann, CT Mayer, SH Naik, S Scheu, G Schreibelt, E Segura, K Seré, T Sparwasser, J Tel, H Xu, M Zenke Guidelines for mouse and human DC generation European Journal of Immunology, 2022-10-27;0(0):. 2022-10-27 [PMID: 36303448] (Mouse)
• MA Meyer, JM Baer, BL Knolhoff, TM Nywening, RZ Panni, X Su, KN Weilbaeche, WG Hawkins, C Ma, RC Fields, DC Linehan, GA Challen, R Faccio, RL Aft, DG DeNardo Breast and pancreatic cancer interrupt IRF8-dependent dendritic cell development to overcome immune surveillance Nat Commun, 2018-03-28;9(1):1250. 2018-03-28 [PMID: 29593283] (Mouse)
• Chen Y, Chang S, Chen T, Lee C Efficient Generation of Plasmacytoid Dendritic Cell from Common Lymphoid Progenitors by Flt3 Ligand. PLoS ONE, 2015-08-11;10(8):e0135217. 2015-08-11 [PMID: 26263178] (Mouse)
• Andersson S, Svensson M, Erlandsson M, Dehlin M, Andersson K, Bokarewa M Activation of Fms-like tyrosine kinase 3 signaling enhances survivin expression in a mouse model of rheumatoid arthritis. PLoS ONE, 2012-10-17;7(10):e47668. 2012-10-17 [PMID: 23082191] (Mouse)

FAQs for Flt-3 Ligand/FLT3L (DY427). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Flt3l