Mouse Flt-3 Ligand/FLT3L DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse Flt-3 Ligand/FLT3L DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Flt3l

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Flt-3 Ligand/FLT3L DuoSet ELISA, 15 Plate

• FL
• FLG3L
• Flt3 ligand
• Flt-3 Ligand
• Flt3L
• FLT3LG
• fms-related tyrosine kinase 3 ligand
• SL cytokine

Background

Flt-3 Ligand, also known as FL, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Mature human Flt-3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. Within the ECD, human Flt-3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt-3 Ligand, respectively. Human and mouse Flt-3 Ligand show cross-species activity. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates. Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain. Alternate splicing of human Flt-3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine domain. Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation. Animal studies also show Flt-3 Ligand to reduce the severity of experimentally induced allergic inflammation.

Publications for Flt-3 Ligand/FLT3L (DY427)(6)

Publications using DY427

• Chun, D;Park, J;Lee, S;Kim, HJ;Park, JE;Kang, SJ; Flt3L enhances clonal diversification and selective expansion of intratumoral CD8+ T cells while differentiating into effector-like cells Cell reports 2024-11-30 [PMID: 39616612] (Human, Mouse, Transgenic Mouse, Xenograft)
• Yu, CI;Maser, R;Marches, F;Banchereau, J;Palucka, K; Long-term engraftment of adult hematopoietic progenitors in a novel model of humanized mice bioRxiv : the preprint server for biology 2023-10-03 [PMID: 37873457] (Mouse)
• MB Lutz, S Ali, C Audiger, SE Autenrieth, L Berod, V Bigley, L Cyran, M Dalod, J Dörrie, D Dudziak, G Flórez-Gra, L Giusiano, GJ Godoy, M Heuer, AB Krug, CHK Lehmann, CT Mayer, SH Naik, S Scheu, G Schreibelt, E Segura, K Seré, T Sparwasser, J Tel, H Xu, M Zenke Guidelines for mouse and human DC generation European Journal of Immunology, 2022-10-27;0(0):. 2022-10-27 [PMID: 36303448] (Mouse)
• MA Meyer, JM Baer, BL Knolhoff, TM Nywening, RZ Panni, X Su, KN Weilbaeche, WG Hawkins, C Ma, RC Fields, DC Linehan, GA Challen, R Faccio, RL Aft, DG DeNardo Breast and pancreatic cancer interrupt IRF8-dependent dendritic cell development to overcome immune surveillance Nat Commun, 2018-03-28;9(1):1250. 2018-03-28 [PMID: 29593283] (Mouse)
• Chen Y, Chang S, Chen T, Lee C Efficient Generation of Plasmacytoid Dendritic Cell from Common Lymphoid Progenitors by Flt3 Ligand. PLoS ONE, 2015-08-11;10(8):e0135217. 2015-08-11 [PMID: 26263178] (Mouse)
• Andersson S, Svensson M, Erlandsson M, Dehlin M, Andersson K, Bokarewa M Activation of Fms-like tyrosine kinase 3 signaling enhances survivin expression in a mouse model of rheumatoid arthritis. PLoS ONE, 2012-10-17;7(10):e47668. 2012-10-17 [PMID: 23082191] (Mouse)

Bioinformatics

• Gene Symbol: Flt3l