Mouse Complement Component C5a DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse Complement Component C5a DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: C5

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Complement Component C5a DuoSet ELISA, 15 Plate

• C5
• C5A
• Complement Component C5a
• HE

Background

Mouse Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as Anaphylatoxins. C5a is a 77 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain. Mouse C5a has four alpha -helices, plus three intrachain disulfide bonds that create a triple loop structure. In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg77 molecule. Mouse C5a shares 60% and 82% aa sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells. Alternatively, it may also bind to a nonsignaling GPCR termed C5L2 whose function is yet to be determined.

Publications for Complement Component C5a (DY2150)(42)

Publications using DY2150

• Schwab, AD;Wyatt, TA;Schanze, OW;Nelson, AJ;Gleason, AM;Duryee, MJ;Mosley, DD;Thiele, GM;Mikuls, TR;Poole, JA; Lung-delivered IL-10 mitigates Lung inflammation induced by repeated endotoxin exposures in male mice Physiological reports 2025-02-01 [PMID: 39980189] (Mouse)
• Li, Y;Jacques, S;Gaikwad, H;Wang, G;Banda, NK;Holers, VM;Scheinman, RI;Tomlinson, S;Moghimi, SM;Simberg, D; Inhibition of acute complement responses towards bolus-injected nanoparticles using targeted short-circulating regulatory proteins Nature nanotechnology 2023-10-05 [PMID: 37798566] (Mouse)
• NC Teske, JY Engelen-Le, S Dyckhoff-S, HW Pfister, M Klein, D van de Bee, CK Kirschning, U Koedel, MC Brouwer The role of plasminogen activator inhibitor-2 in pneumococcal meningitis Acta neuropathologica communications, 2022-10-29;10(1):155. 2022-10-29 [PMID: 36309755] (Mouse)
• G Arora, GE Lynn, X Tang, CE Rosen, D Hoornstra, A Sajid, JW Hovius, NW Palm, AM Ring, E Fikrig CD55 Facilitates Immune Evasion by Borrelia crocidurae, an Agent of Relapsing Fever MBio, 2022-08-29;0(0):e0116122. 2022-08-29 [PMID: 36036625] (Mouse)
• M Llorián-Sa, EM Byrne, M Szczepan, K Little, M Chen, H Xu Complement activation contributes to subretinal fibrosis through the induction of epithelial-to-mesenchymal transition (EMT) in retinal pigment epithelial cells Journal of Neuroinflammation, 2022-07-14;19(1):182. 2022-07-14 [PMID: 35831910] (Mouse)
• K Carvalho, ND Schartz, G Balderrama, HY Liang, SH Chu, P Selvan, A Gomez-Arbo, TJ Petrisko, MI Fonseca, A Mortazavi, AJ Tenner Modulation of C5a-C5aR1 signaling alters the dynamics of AD progression Journal of Neuroinflammation, 2022-07-11;19(1):178. 2022-07-11 [PMID: 35820938] (Mouse)
• P Ding, Y Xu, L Li, X Lv, L Li, J Chen, D Zhou, X Wang, Q Wang, W Zhang, T Liao, QH Ji, QY Lei, W Hu Intracellular complement C5a/C5aR1 stabilizes beta-catenin to promote colorectal tumorigenesis Cell Reports, 2022-05-31;39(9):110851. 2022-05-31 [PMID: 35649359] (Mouse)
• Y Tachida, S Miura, Y Muto, H Takuwa, N Sahara, A Shindo, Y Matsuba, T Saito, N Taniguchi, Y Kawaguchi, H Tomimoto, T Saido, S Kitazume Endothelial expression of human amyloid precursor protein leads to amyloid beta in the blood and induces cerebral amyloid angiopathy in knock-in mice The Journal of Biological Chemistry, 2022-03-31;0(0):101880. 2022-03-31 [PMID: 35367207] (Mouse)
• AK Bongoni, IB Vikstrom, JL McRae, EJ Salvaris, N Fisicaro, MJ Pearse, S Wymann, T Rowe, AB Morelli, MP Hardy, PJ Cowan A potent truncated form of human soluble CR1 is protective in a mouse model of renal ischemia-reperfusion injury Scientific Reports, 2021-11-08;11(1):21873. 2021-11-08 [PMID: 34750424] (Mouse)
• TR Mikuls, R Gaurav, GM Thiele, BR England, MG Wolfe, BP Shaw, KL Bailey, TA Wyatt, AJ Nelson, MJ Duryee, CD Hunter, D Wang, DJ Romberger, DP Ascherman, JA Poole The impact of airborne endotoxin exposure on rheumatoid arthritis-related joint damage, autoantigen expression, autoimmunity, and lung disease International immunopharmacology, 2021-08-27;100(0):108069. 2021-08-27 [PMID: 34461491] (Mouse)
• A Doni, R Parente, I Laface, E Magrini, C Cunha, FS Colombo, JF Lacerda, A Campos, SN Mapelli, F Petroni, R Porte, T Schorn, A Inforzato, T Mercier, K Lagrou, J Maertens, JD Lambris, B Bottazzi, C Garlanda, M Botto, A Carvalho, A Mantovani Serum amyloid P component is an essential element of resistance against Aspergillus fumigatus Nature Communications, 2021-06-18;12(1):3739. 2021-06-18 [PMID: 34145258] (Mouse)
• SA Appiah, CL Foxx, D Langgartne, A Palmer, CA Zambrano, S Braumüller, EJ Schaefer, U Wachter, BL Elam, P Radermache, CE Stamper, JD Heinze, SN Salazar, AK Luthens, AL Arnold, SO Reber, M Huber-Lang, CA Lowry, R Halbgebaue Evaluation of the gut microbiome in association with biological signatures of inflammation in murine polytrauma and shock Scientific Reports, 2021-03-23;11(1):6665. 2021-03-23 [PMID: 33758228] (Mouse)
• RS Aquino, A Hayashida, PW Park Host syndecan-1 promotes listeriosis by inhibiting intravascular neutrophil extracellular traps PLoS Pathog., 2020-05-26;16(5):e1008497. 2020-05-26 [PMID: 32453780] (Mouse)
• FH Brennan, T Jogia, ER Gillespie, LV Blomster, XX Li, B Nowlan, GM Williams, E Jacobson, GW Osborne, FA Meunier, SM Taylor, KE Campbell, KP MacDonald, JP Levesque, TM Woodruff, MJ Ruitenberg Complement receptor C3aR1 controls neutrophil mobilization following spinal cord injury through physiological antagonism of CXCR2 JCI Insight, 2019-05-02;4(9):. 2019-05-02 [PMID: 31045582] (Mouse)
• V Escamilla-, A Solorio-Ro, M Uribe-Ramí, O Lozano, S Lucas, A Chagolla-L, R Winkler, A De Vizcaya Plasma protein adsorption on Fe3O4-PEG nanoparticles activates the complement system and induces an inflammatory response Int J Nanomedicine, 2019-03-25;14(0):2055-2067. 2019-03-25 [PMID: 30988608] (Mouse)
• C Hu, L Li, P Ding, L Li, X Ge, L Zheng, X Wang, J Wang, W Zhang, N Wang, H Gu, F Zhong, M Xu, R Rong, T Zhu, W Hu Complement Inhibitor CRIg/FH Ameliorates Renal Ischemia Reperfusion Injury via Activation of PI3K/AKT Signaling J. Immunol., 2018-11-14;0(0):. 2018-11-14 [PMID: 30429287] (Mouse)
• F Fattahi, MW Russell, EA Malan, M Parlett, E Abe, FS Zetoune, PA Ward Harmful Roles of TLR3 and TLR9 in Cardiac Dysfunction Developing during Polymicrobial Sepsis Biomed Res Int, 2018-09-30;2018(0):4302726. 2018-09-30 [PMID: 30364002] (Mouse)
• B Wang, H Xu, J Li, HM Gao, YH Xing, Z Lin, HJ Li, YQ Wang, SH Cao Complement depletion with cobra venom factor alleviates acute hepatic injury induced by ischemia?reperfusion Mol Med Rep, 2018-09-14;0(0):. 2018-09-14 [PMID: 30221740] (Rat)
• R Spirig, IK Campbell, S Koernig, CG Chen, BJB Lewis, R Butcher, I Muir, S Taylor, J Chia, D Leong, J Simmonds, P Scotney, P Schmidt, L Fabri, A Hofmann, M Jordi, MO Spycher, S Cattepoel, J Brasseit, C Panousis, T Rowe, DR Branch, A Baz Morell, F Käsermann, AW Zuercher rIgG1 Fc Hexamer Inhibits Antibody-Mediated Autoimmune Disease via Effects on Complement and Fc?Rs J. Immunol., 2018-03-12;0(0):. 2018-03-12 [PMID: 29531170] (Mouse)
• JD Boehme, S Stegemann-, A Autengrube, N Peters, J Wissing, L Jänsch, A Jeron, D Bruder Chronic lung inflammation primes humoral immunity and augments antipneumococcal resistance Sci Rep, 2017-07-10;7(1):4972. 2017-07-10 [PMID: 28694492] (Mouse)
• Y Wang, P Lai, X Chen, C He, X Huang, S Geng, C Luo, S Wu, W Ling, L Zhong, Z Lu, P Li, J Weng, X Du Attenuation of cGVHD by C5a/C5aR blockade is associated with increased frequency of Treg Sci Rep, 2017-06-15;7(1):3603. 2017-06-15 [PMID: 28620195] (Mouse)
• S Bergdolt, A Kovtun, Y Hägele, A Liedert, T Schinke, M Amling, M Huber-Lang, A Ignatius Osteoblast-specific overexpression of complement receptor C5aR1 impairs fracture healing PLoS ONE, 2017-06-14;12(6):e0179512. 2017-06-14 [PMID: 28614388] (Mouse)
• JH Sheen, MG Strainic, J Liu, W Zhang, Z Yi, ME Medof, PS Heeger TLR-Induced Murine Dendritic Cell (DC) Activation Requires DC-Intrinsic Complement J. Immunol., 2017-05-24;0(0):. 2017-05-24 [PMID: 28539427] (Mouse)
• AK Bongoni, B Lu, EJ Salvaris, V Roberts, D Fang, JL McRae, N Fisicaro, KM Dwyer, PJ Cowan Overexpression of Human CD55 and CD59 or Treatment with Human CD55 Protects against Renal Ischemia-Reperfusion Injury in Mice J. Immunol., 2017-05-12;0(0):. 2017-05-12 [PMID: 28500075] (Mouse)
• LG Coulthard, OA Hawksworth, R Li, A Balachandr, JD Lee, F Sepehrband, N Kurniawan, A Jeanes, DG Simmons, E Wolvetang, TM Woodruff Complement C5aR1 Signaling Promotes Polarization and Proliferation of Embryonic Neural Progenitor Cells through PKC? J. Neurosci., 2017-04-28;0(0):. 2017-04-28 [PMID: 28455369] (Mouse)
• KM Cunnion, NK Krishna, HK Pallera, A Pineros-Fe, MG Rivera, PS Hair, BP Lassiter, R Huyck, MA Clements, AF Hood, GT Rodeheaver, PS Cottler, JL Nadler, AD Dobrian Complement Activation and STAT4 Expression Are Associated with Early Inflammation in Diabetic Wounds PLoS ONE, 2017-01-20;12(1):e0170500. 2017-01-20 [PMID: 28107529] (Mouse)
• High-fat diet-induced complement activation mediates intestinal inflammation and neoplasia, independent of obesity Mol Cancer Res, 2016-08-17;0(0):. 2016-08-17 [PMID: 27535705] (Mouse)
• The Complement System Component C5a Produces Thermal Hyperalgesia via Macrophage-to-Nociceptor Signaling That Requires NGF and TRPV1 J Neurosci, 2016-05-04;36(18):5055-70. 2016-05-04 [PMID: 27147658] (Mouse)
• Sun D, Zhang M, Liu G, Wu H, Zhu X, Zhou H, Shi M Real-Time Imaging of Interactions of Neutrophils with Cryptococcus neoformans Demonstrates a Crucial Role of Complement C5a-C5aR Signaling. Infect Immun, 2015-10-26;84(1):216-29. 2015-10-26 [PMID: 26502909] (Mouse)
• Brennan F, Gordon R, Lao H, Biggins P, Taylor S, Franklin R, Woodruff T, Ruitenberg M The Complement Receptor C5aR Controls Acute Inflammation and Astrogliosis following Spinal Cord Injury. J Neurosci, 2015-04-22;35(16):6517-31. 2015-04-22 [PMID: 25904802] (Mouse)
• Chou C, Lai S, Ho C, Lin W, Chen C, Lee P, Peng F, Kuo S, Wu S, Lai H Lysophosphatidic acid alters the expression profiles of angiogenic factors, cytokines, and chemokines in mouse liver sinusoidal endothelial cells. PLoS ONE, 2015-03-30;10(3):e0122060. 2015-03-30 [PMID: 25822713] (Mouse)
• Price P, Banki Z, Scheideler A, Stoiber H, Verschoor A, Sutter G, Lehmann M Complement component C5 recruits neutrophils in the absence of C3 during respiratory infection with modified vaccinia virus Ankara. J Immunol, 2014-12-29;194(3):1164-8. 2014-12-29 [PMID: 25548218] (Mouse)
• Sharif O, Gawish R, Warszawska J, Martins R, Lakovits K, Hladik A, Doninger B, Brunner J, Korosec A, Schwarzenbacher R, Berg T, Kralovics R, Colinge J, Mesteri I, Gilfillan S, Salmaggi A, Verschoor A, Colonna M, Knapp S The triggering receptor expressed on myeloid cells 2 inhibits complement component 1q effector mechanisms and exerts detrimental effects during pneumococcal pneumonia. PLoS Pathog, 2014-06-12;10(6):e1004167. 2014-06-12 [PMID: 24945405] (Mouse)
• Ehrnthaller , Christia, Huber-Lang , Markus, Nilsson , Per, Bindl , Ronny, Redeker , Simon, Recknagel , Stefan, Rapp , Anna, Mollnes , Tom, Amling , Michael, Gebhard , Florian, Ignatius , Anita Complement C3 and C5 deficiency affects fracture healing. PLoS ONE, 2013-11-18;8(11):e81341. 2013-11-18 [PMID: 24260573] (Mouse)
• Gauvreau D, Gupta A, Fisette A, Tom F, Cianflone K Deficiency of C5L2 increases macrophage infiltration and alters adipose tissue function in mice. PLoS ONE, 2013-04-22;8(4):e60795. 2013-04-22 [PMID: 23630572] (Mouse)
• Kwan WH, Hashimoto D, Paz-Artal E Antigen-presenting cell-derived complement modulates graft-versus-host disease. J. Clin. Invest., 2012-05-15;122(6):2234-8. 2012-05-15 [PMID: 22585573] (Mouse)
• Jang JH, Liang D, Kido K Increased local concentration of complement C5a contributes to incisional pain in mice. J Neuroinflammation, 2011-07-07;8(0):80. 2011-07-07 [PMID: 21736743] (Mouse)
• Vieyra M, Leisman S, Raedler H, Kwan WH, Yang M, Strainic MG, Medof ME, Heeger PS Complement regulates CD4 T-cell help to CD8 T cells required for murine allograft rejection. Am. J. Pathol., 2011-06-23;179(2):766-74. 2011-06-23 [PMID: 21704012] (Mouse)
• O'Brien KB, Morrison TE, Dundore DY A protective role for complement C3 protein during pandemic 2009 H1N1 and H5N1 influenza A virus infection. PLoS ONE, 2011-03-09;6(3):e17377. 2011-03-09 [PMID: 21408070] (Mouse)
• Heimbach L, Li Z, Berkowitz P The C5a Receptor on Mast Cells Is Critical for the Autoimmune Skin-blistering Disease Bullous Pemphigoid. J. Biol. Chem., 2011-03-10;286(17):15003-9. 2011-03-10 [PMID: 21393236] (Mouse)
• Dahlke K, Wrann CD, Sommerfeld O, Sossdorf M, Recknagel P, Sachse S, Winter SW, Klos A, Stahl GL, Ma YX, Claus RA, Reinhart K, Bauer M, Riedemann NC Distinct Different Contributions of the Alternative and Classical Complement Activation Pathway for the Innate Host Response during Sepsis. J. Immunol., 2011-01-24;186(5):3066-3075. 2011-01-24 [PMID: 21263075] (Mouse)
• Subramaniam KS, Datta K, Quintero E, Manix C, Marks MS, Pirofski LA The absence of serum IgM enhances the susceptibility of mice to pulmonary challenge with Cryptococcus neoformans. J. Immunol., 2010-04-19;184(10):5755-67. 2010-04-19 [PMID: 20404271] (Mouse)

FAQs for Complement Component C5a (DY2150). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: C5