Mouse CCL12/MCP-5 Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse CCL12/MCP-5 Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse MCP-5 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse MCP-5 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse MCP-5 and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse MCP-5 showed linear curves that were parallel to the standard curves obta...ined using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse MCP-5.
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• Specificity: Natural and recombinant mouse MCP-5
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Ccl12

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse CCL12/MCP-5 Quantikine ELISA Kit

• C-C motif chemokine 12
• CCL12
• chemokine (C-C motif) ligand 12
• MCP5
• MCP-5
• Scya12

Background

Mouse MCP-5, also known as CCL12, encodes a 104 amino acid (aa) precursor protein with a 22 aa hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. MCP-5 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, MCP-5 expression is also induced in activated macrophages and mast cells.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CCL12/MCP-5 (MCC120)(6)

Publications using MCC120

• Sha, L;Li, J;Shen, H;Wang, Q;Meng, P;Zhang, X;Deng, Y;Zhu, W;Xu, Q; LHPP-mediated inorganic pyrophosphate hydrolysis-driven lysosomal acidification in astrocytes regulates adult neurogenesis Cell reports 2023-08-11 [PMID: 37573508] (Mouse)
• W Li, X Zhang, C Zhang, J Yan, X Hou, S Du, C Zeng, W Zhao, B Deng, DW McComb, Y Zhang, DD Kang, J Li, WE Carson, Y Dong Biomimetic nanoparticles deliver mRNAs encoding costimulatory receptors and enhance T cell mediated cancer immunotherapy Nature Communications, 2021-12-14;12(1):7264. 2021-12-14 [PMID: 34907171] (Mouse)
• Alm AS, Li K, Yang D, Andersson R, Lu Y, Wang X Varying susceptibility of pulmonary cytokine production to lipopolysaccharide in mice. Cytokine, 2009-12-29;49(3):256-63. 2009-12-29 [PMID: 20042347] (Mouse)
• Oh SY, Zheng T, Bailey ML, Barber DL, Schroeder JT, Kim YK, Zhu Z Src homology 2 domain-containing inositol 5-phosphatase 1 deficiency leads to a spontaneous allergic inflammation in the murine lung. J. Allergy Clin. Immunol., 2006-10-23;119(1):123-31. 2006-10-23 [PMID: 17208593] (Mouse)
• Weighardt H, Kaiser-Moore S, Schlautkotter S, Rossmann-Bloeck T, Schleicher U, Bogdan C, Holzmann B Type I IFN modulates host defense and late hyperinflammation in septic peritonitis. J. Immunol., 2006-10-15;177(8):5623-30. 2006-10-15 [PMID: 17015750] (Mouse)
• Stathopoulos GT, Zhu Z, Everhart MB, Kalomenidis I, Lawson WE, Bilaceroglu S, Peterson TE, Mitchell D, Yull FE, Light RW, Blackwell TS Nuclear factor-kappaB affects tumor progression in a mouse model of malignant pleural effusion. Am. J. Respir. Cell Mol. Biol., 2005-10-06;34(2):142-50. 2005-10-06 [PMID: 16210694] (Mouse)

FAQs for CCL12/MCP-5 (MCC120). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Ccl12