Mouse BAFF/BLyS/TNFSF13B Quantikine ELISA Kit

OTULIN Deficiency in Myeloid Cells Leads to Neutrophil Infiltration, Multi-organ Inflammation, Hyper-Immunoglobulinemia, and Autoimmunity(A and B) Micrographs of H&E and Masson’s trichome stained sections of livers and spleens from 4- to 9-months-old LysMCre-Otulinflox mice reveal (A) infiltration in and fibrosis (right, arrowheads) of liver parenchyma and (B) distorted spleen architecture with germinal center activation (left, arrowheads) and fibrosis (right, arrowheads) in LysMCre-OtulinLacZ/flox mice. Scale bars, 200 μm. Micrographs are representative of five mice of each genotype from two independent experiments.(C and D) Flow cytometry analysis of total cellular infiltrate (CD45.1+ and CD45.2+) in peritoneal lavage (PL), spleen, liver, and kidney of LysMCre-Otulinflox mice presented as (C) representative dot plots of neutrophils with percent of cells in gate indicated and (D) total cell number of neutrophils, macrophages, and CD8+ T cells.(E) Concentrations of immunoglobulins in serum from 4- to 9-months-old LysMCre-Otulinflox mice.(F) ELISA analysis of serum autoantibody reactivity to ENA, dsDNA, and Smith antigen from 3- to 9-months-old LysMCre-Otulinflox mice.(G) ELISA analysis of serum B cell activating factor (BAFF) concentration from 3- to 9-months-old LysMCre-Otulinflox mice.(E–G) Data are mean of two technical replicas.(D–F) Data are presented as mean ± SEM, and n represents number of mice.See also Figure S5. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27523608), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse BAFF/BLyS/TNFSF13B Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse BAFF/BLyS Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse BAFF/BLyS in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse BAFF/BLyS and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse BAFF/BLyS ...showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse BAFF/BLyS.
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• Specificity: Natural and recombinant mouse BAFF/BLyS
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Tnfsf13b

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse BAFF/BLyS/TNFSF13B Quantikine ELISA Kit

• ApoL related ligand TALL-1
• B lymphocyte stimulator
• BAFF
• BAFFB-cell activating factor
• B-cell-activating factor
• BLyS
• BLYSB-lymphocyte stimulator
• CD257 antigen
• CD257
• Dendritic cell-derived TNF-like molecule
• DTL
• TALL1
• TALL-1delta BAFF
• TALL1Delta4 BAFF
• THANK
• TNF- and APOL-related leukocyte expressed ligand 1
• TNF and ApoL-related leukocyte expressed ligand 1
• TNF homolog that activates apoptosis
• TNFSF13B
• TNFSF20
• tumor necrosis factor (ligand) superfamily, member 13b
• tumor necrosis factor (ligand) superfamily, member 20
• tumor necrosis factor ligand superfamily member 13B
• tumor necrosis factor superfamily, member 13b
• tumor necrosis factor-like protein ZTNF4
• ZTNF4

Background

BAFF (B-cell activating factor), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a TNF superfamily ligand that plays a critical role in the development and survival of B lineage cells. It can be expressed as a homotrimer or as a heteromer in association with the related protein APRIL. It is produced by many hematopoietic cell types, and a soluble form can be released by activated neutrophils. Both BAFF and APRIL signal through the receptors BCMA and TACI, and BAFF additionally signals through BAFF R. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for BAFF/BLyS/TNFSF13B (MBLYS0)(30)

Publications using MBLYS0

• Qian, Y;Kang, Z;Zhao, L;Chen, H;Zhou, C;Gao, Q;Wang, Z;Liu, Q;Cui, Y;Li, X;Chen, Y;Zou, T;Fang, J; Berberine might block colorectal carcinogenesis by inhibiting the regulation of B-cell function by Veillonella parvula Chinese medical journal 2023-08-09 [PMID: 37553874] (Mouse)
• BF Brian, ML Sauer, JT Greene, SE Senevirath, AJ Lindstedt, OL Funk, BL Ruis, LA Ramirez, JL Auger, WL Swanson, MG Nunez, BS Moriarity, CA Lowell, BA Binstadt, TS Freedman A dominant function of LynB kinase in preventing autoimmunity Science Advances, 2022-04-22;8(16):eabj5227. 2022-04-22 [PMID: 35452291] (Mouse)
• S Chappaz, K McArthur, L Kealy, CW Law, M Tailler, RM Lane, A Lieschke, ME Ritchie, KL Good-Jacob, A Strasser, BT Kile Homeostatic apoptosis prevents competition-induced atrophy in follicular B cells Cell Reports, 2021-07-20;36(3):109430. 2021-07-20 [PMID: 34289356] (Mouse)
• X Peng, Z Yong, W Xiaoyan, C Yuanshan, W Guangzhu, L Xuehuan Mechanism of Graft Damage Caused by NTPDase1-activated Macrophages in Acute Antibody-Mediated Rejection Transplant. Proc., 2020-08-06;0(0):. 2020-08-06 [PMID: 32773283] (Mouse)
• SH Khiew, D Jain, J Chen, J Yang, D Yin, JS Young, A Dent, R Sciammas, ML Alegre, AS Chong Transplantation tolerance modifies donor-specific B cell fate to suppress de novo alloreactive B cells J. Clin. Invest., 2020-07-01;0(0):. 2020-07-01 [PMID: 32452834] (Mouse)
• MR Edwards, R Dai, B Heid, C Cowan, SR Werre, T Cecere, SA Ahmed Low-dose 17&alpha-ethinyl estradiol (EE) exposure exacerbates lupus renal disease and modulates immune responses to TLR7/9 agonists in genetically autoimmune-prone mice Sci Rep, 2020-03-23;10(1):5210. 2020-03-23 [PMID: 32251357] (Mouse)
• Y Nakamura, M Abe, K Kawasaki, T Miyake, T Watanabe, O Yoshida, M Hirooka, B Matsuura, Y Hiasa Depletion of B cell-activating factor attenuates hepatic fat accumulation in a murine model of nonalcoholic fatty liver disease Sci Rep, 2019-01-30;9(1):977. 2019-01-30 [PMID: 30700810] (Mouse)
• AS Wilhelmson, M Lantero Ro, A Stubelius, P Fogelstran, I Johansson, MB Buechler, S Lianoglou, VN Kapoor, ME Johansson, JB Fagman, A Duhlin, P Tripathi, A Camponesch, BT Porse, AG Rolink, H Nissbrandt, SJ Turley, H Carlsten, IL Mårtensson, MCI Karlsson, Å Tivesten Testosterone is an endogenous regulator of BAFF and splenic B cell number Nat Commun, 2018-05-25;9(1):2067. 2018-05-25 [PMID: 29802242] (Mouse)
• W Alturaiki, AJ McFarlane, K Rose, R Corkhill, PS McNamara, J Schwarze, BF Flanagan Expression of the B cell differentiation factor BAFF and chemokine CXCL13 in a murine model of Respiratory Syncytial Virus infection Cytokine, 2018-02-01;0(0):. 2018-02-01 [PMID: 29396045] (Mouse)
• LH Thai, S Le Gallou, A Robbins, E Crickx, T Fadeev, Z Zhou, N Cagnard, J Mégret, C Bole, JC Weill, CA Reynaud, M Mahévas BAFF and CD4T cells are major survival factors for long-lived splenic plasma cells in a B-cell-depletion context Blood, 2018-01-29;0(0):. 2018-01-29 [PMID: 29378696] (Mouse)
• KM Azzam, JH Madenspach, DW Cain, L Lai, KM Gowdy, P Rai, K Janardhan, N Clayton, W Cunningham, H Jensen, PS Patel, JF Kearney, GA Taylor, MB Fessler Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces JCI Insight, 2017-08-17;2(16):. 2017-08-17 [PMID: 28814662] (Mouse)
• S Sanges, M Jendoubi, N Kavian, C Hauspie, S Speca, JC Crave, T Guerrier, G Lefèvre, V Sobanski, A Savina, E Hachulla, PY Hatron, M Labalette, F Batteux, S Dubucquoi, D Launay B Cell Homeostasis and Functional Properties Are Altered in an Hypochlorous Acid-Induced Murine Model of Systemic Sclerosis Front Immunol, 2017-02-07;8(0):53. 2017-02-07 [PMID: 28223983] (Mouse)
• FM Verhamme, LJ Seys, EG De Smet, S Provoost, W Janssens, D Elewaut, GF Joos, GG Brusselle, KR Bracke Elevated GDF-15 contributes to pulmonary inflammation upon cigarette smoke exposure Mucosal Immunol, 2017-02-01;0(0):. 2017-02-01 [PMID: 28145442] (Mouse)
• The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity Cell, 2016-08-11;0(0):. 2016-08-11 [PMID: 27523608] (Mouse)
• BANK1 Regulates IgG Production in a Lupus Model by Controlling TLR7-Dependent STAT1 Activation PLoS ONE, 2016-05-26;11(5):e0156302. 2016-05-26 [PMID: 27228057] (Mouse)
• MRL Strains Have a BAFFR Mutation without Functional Consequence PLoS ONE, 2016-05-05;11(5):e0154518. 2016-05-05 [PMID: 27149280] (Mouse)
• Li S, Abbas A, Solomon L, Groux G, DeKoter R Nfkb1 activation by the E26 transformation-specific transcription factors PU.1 and Spi-B promotes Toll-like receptor-mediated splenic B cell proliferation. Mol Cell Biol, 2015-03-02;35(9):1619-32. 2015-03-02 [PMID: 25733685] (Mouse)
• Neill , Daniel R, Saint , Gemma L, Bricio-Moreno , Laura, Fothergill , Joanne L, Southern , Kevin W, Winstanley , Craig, Christmas , Stephen, Slupsky , Joseph R, McNamara , Paul S, Kadioglu , Aras, Flanagan , Brian F The B lymphocyte differentiation factor (BAFF) is expressed in the airways of children with CF and in lungs of mice infected with Pseudomonas aeruginosa. PLoS ONE, 2014-05-21;9(5):e95892. 2014-05-21 [PMID: 24847941] (Mouse)
• Uslu K, Coleman A, Allman W, Katsenelson N, Bram R, Alugupalli K, Akkoyunlu M Impaired B cell receptor signaling is responsible for reduced TACI expression and function in X-linked immunodeficient mice. J Immunol, 2014-03-19;192(8):3582-95. 2014-03-19 [PMID: 24646744] (Mouse, Transgenic Mouse)
• Kotani Y, Kunisawa J, Suzuki Y, Sato I, Saito T, Toba M, Kohda N, Kiyono H Role of Lactobacillus pentosus Strain b240 and the Toll-like receptor 2 axis in Peyer's patch dendritic cell-mediated immunoglobulin A enhancement. PLoS ONE, 2014-03-14;9(3):e91857. 2014-03-14 [PMID: 24632732] (Mouse)
• Patton D, Plumb A, Redpath S, Osborne L, Perona-Wright G, Abraham N The development and survival but not function of follicular B cells is dependent on IL-7Ralpha Tyr449 signaling. PLoS ONE, 2014-02-13;9(2):e88771. 2014-02-13 [PMID: 24551160] (Mouse)
• Kikuchi , Yosuke, Kunitoh-Asari , Ayami, Hayakawa , Katsuyuk, Imai , Shinjiro, Kasuya , Kenji, Abe , Kimio, Adachi , Yu, Fukudome , Shin-Ich, Takahashi , Yoshimas, Hachimura , Satoshi Oral administration of Lactobacillus plantarum strain AYA enhances IgA secretion and provides survival protection against influenza virus infection in mice. PLoS ONE, 2014-01-22;9(1):e86416. 2014-01-22 [PMID: 24466081] (Mouse)
• van Til N, Sarwari R, Visser T, Hauer J, Lagresle-Peyrou C, van der Velden G, Malshetty V, Cortes P, Jollet A, Danos O, Cassani B, Zhang F, Thrasher A, Fontana E, Poliani P, Cavazzana M, Verstegen M, Villa A, Wagemaker G Recombination-activating gene 1 (Rag1)-deficient mice with severe combined immunodeficiency treated with lentiviral gene therapy demonstrate autoimmune Omenn-like syndrome. J Allergy Clin Immunol, 2013-12-09;133(4):1116-23. 2013-12-09 [PMID: 24332219] (Mouse)
• Inhibition of hedgehog signaling for the treatment of murine sclerodermatous chronic graft-versus-host disease. Blood, 2012-08-22;120(14):2909-17. 2012-08-22 [PMID: 22915638] (Mouse)
• Johnson AC, Davison LM, Giltiay NV, Vareechon C, Li X, Jorgensen TN Lack of T cells in Act1-deficient mice results in elevated IgM-specific autoantibodies but reduced lupus-like disease. Eur. J. Immunol., 2012-07-01;42(7):1695-705. 2012-07-01 [PMID: 22585710] (Mouse)
• McCarthy DD, Kujawa J, Wilson C Mice overexpressing BAFF develop a commensal flora-dependent, IgA-associated nephropathy. J. Clin. Invest., 2011-09-01;121(10):3991-4002. 2011-09-01 [PMID: 21881212] (Mouse)
• Giltiay NV, Lu Y, Allman D, Jorgensen TN, Li X The adaptor molecule Act1 regulates BAFF responsiveness and self-reactive B cell selection during transitional B cell maturation. J. Immunol., 2010-06-11;185(1):99-109. 2010-06-11 [PMID: 20543113] (Mouse)
• Notley CA, Baker N, Ehrenstein MR Secreted IgM enhances B cell receptor signaling and promotes splenic but impairs peritoneal B cell survival. J. Immunol., 2010-03-05;184(7):3386-93. 2010-03-05 [PMID: 20207998] (Mouse)
• Li Z, Wang H, Xue L, Shin DM, Roopenian D, Xu W, Qi CF, Sangster MY, Orihuela CJ, Tuomanen E, Rehg JE, Cui X, Zhang Q, Morse HC, Morris SW Emu-BCL10 mice exhibit constitutive activation of both canonical and noncanonical NF-kappaB pathways generating marginal zone (MZ) B-cell expansion as a precursor to splenic MZ lymphoma. Blood, 2009-08-20;114(19):4158-68. 2009-08-20 [PMID: 19696203] (Mouse)
• Kahn P, Ramanujam M, Bethunaickan R, Huang W, Tao H, Madaio MP, Factor SM, Davidson A Prevention of murine antiphospholipid syndrome by BAFF blockade. Arthritis Rheum., 2008-09-01;58(9):2824-34. 2008-09-01 [PMID: 18759321] (Mouse)

Bioinformatics

• Gene Symbol: Tnfsf13b