Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA, 5 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Tnfsf13b

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA, 5 Plate

• ApoL related ligand TALL-1
• B lymphocyte stimulator
• BAFF
• BAFFB-cell activating factor
• B-cell-activating factor
• BLyS
• BLYSB-lymphocyte stimulator
• CD257 antigen
• CD257
• Dendritic cell-derived TNF-like molecule
• DTL
• TALL1
• TALL-1delta BAFF
• TALL1Delta4 BAFF
• THANK
• TNF- and APOL-related leukocyte expressed ligand 1
• TNF and ApoL-related leukocyte expressed ligand 1
• TNF homolog that activates apoptosis
• TNFSF13B
• TNFSF20
• tumor necrosis factor (ligand) superfamily, member 13b
• tumor necrosis factor (ligand) superfamily, member 20
• tumor necrosis factor ligand superfamily member 13B
• tumor necrosis factor superfamily, member 13b
• tumor necrosis factor-like protein ZTNF4
• ZTNF4

Background

BAFF (B-cell activating factor), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a TNF superfamily ligand that plays a critical role in the development and survival of B lineage cells. It can be expressed as a homotrimer or as a heteromer in association with the related protein APRIL. It is produced by many hematopoietic cell types, and a soluble form can be released by activated neutrophils. Both BAFF and APRIL signal through the receptors BCMA and TACI, and BAFF additionally signals through BAFF R. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells.

Publications for BAFF/BLyS/TNFSF13B (DY2106-05)(9)

Publications using DY2106-05

• JR Doll, ME Moreno-Fer, TE Stankiewic, JL Wayland, A Wilburn, B Weinhaus, CA Chougnet, D Giordano, M Cappellett, P Presicce, SG Kallapur, N Salomonis, T Tilburgs, S Divanovic BAFF and APRIL counterregulate susceptibility to inflammation-induced preterm birth Cell Reports, 2023-04-05;42(4):112352. 2023-04-05 [PMID: 37027297] (Mouse)
• R Kuley, KE Draves, DH Fuller, NV Giltiay, EA Clark, D Giordano B cell activating factor (BAFF) from neutrophils and dendritic cells is required for protective B cell responses against Salmonella typhimurium infection PLoS ONE, 2021-10-27;16(10):e0259158. 2021-10-27 [PMID: 34705890] (Mouse)
• L Liu, M Akkoyunlu Circulating CD138 enhances disease progression by augmenting autoreactive antibody production in a mouse model of systemic lupus erythematosus The Journal of Biological Chemistry, 2021-08-06;0(0):101053. 2021-08-06 [PMID: 34364875] (Mouse)
• N Azuma, S Omachi, W Hanazawa, A Morimoto, C Sanjoba, Y Matsumoto, W Fujii, Y Goto Variable dependency on BAFF in IgG antibody production during Leishmania infection Parasitol. Int., 2019-10-15;74(0):101997. 2019-10-15 [PMID: 31626986] (Mouse)
• J Dang, X Bian, X Ma, J Li, F Long, S Shan, Q Yuan, Q Xin, Y Li, F Gao, Y Gong, Q Liu ORMDL3 Facilitates the Survival of Splenic B Cells via an ATF6?-Endoplasmic Reticulum Stress-Beclin1 Autophagy Regulatory Pathway J. Immunol., 2017-07-26;0(0):. 2017-07-26 [PMID: 28747345] (Mouse)
• S Omachi, W Fujii, N Azuma, A Morimoto, C Sanjoba, Y Matsumoto, Y Goto B-cell activating factor deficiency suppresses splenomegaly during Leishmania donovani infection Biochem. Biophys. Res. Commun., 2017-06-03;0(0):. 2017-06-03 [PMID: 28583852] (Mouse)
• Silica-Triggered Autoimmunity in Lupus-Prone Mice Blocked by Docosahexaenoic Acid Consumption PLoS ONE, 2016-08-11;11(8):e0160622. 2016-08-11 [PMID: 27513935] (Mouse)
• Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells. Leukemia, 2015-07-03;30(1):163-72. 2015-07-03 [PMID: 26139429] (Mouse)
• Cremasco , Viviana, Woodruff , Matthew, Onder , Lucas, Cupovic , Jovana, Nieves-Bonilla , Janice M, Schildberg , Frank A, Chang , Jonathan, Cremasco , Floriana, Harvey , Christop, Wucherpfennig , Kai, Ludewig , Burkhard, Carroll , Michael, Turley , Shannon B cell homeostasis and follicle confines are governed by fibroblastic reticular cells. Nat Immunol, 2014-08-24;15(10):973-81. 2014-08-24 [PMID: 25151489] (Mouse)

FAQs for BAFF/BLyS/TNFSF13B (DY2106-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Tnfsf13b