Moesin Antibody

Western Blot: Moesin Antibody [NB100-40811] - Analysis of Moesin Antibody using this antibody at 1ug/ml in Human Spleen lysate (35ug protein in RIPA buffer).

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Moesin Antibody Summary

• Description: Novus Biologicals Goat Moesin Antibody (NB100-40811) is a polyclonal antibody validated for use in WB and ELISA. Anti-Moesin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NARDESKKTAND corresponding to internal region according to NP_002435.1.
• Predicted Species: Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MSN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in human and mouse spleen lysates (calculated MW of 67.8 kDa band according to NP_002435.1).
• Control:

  Human Spleen Whole Tissue Lysate (Adult Whole Normal)
  Moesin Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Moesin Antibody

• Membrane-organizing extension spike protein
• moesin

Background

Moesin is a member of the ERM (Ezrin-Radixin-Moesin) protein family, which possesses structural and probably functional relationships; they function as plasma membrane-actin cytoskeleton linkers and participate in the formation of specialized domains of the plasma membrane and are all localized to the submembranous cytoskeleton. Moesin is widely expressed in different tissues in cells, where it is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signalling and for cell movement.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Moesin Antibody (NB100-40811)(1)

Publication using NB100-40811

• Ivetic A, Florey O, Deka J et al. Mutagenesis of the ezrin-radixin-moesin binding domain of L-selectin tail affects shedding, microvillar positioning, and leukocyte tethering. J Biol Chem 2004-08-06 [PMID: 15178693]

FAQs for Moesin Antibody (NB100-40811). (Showing 1 - 1 of 1 FAQ).

1. I am looking to use shRNA to inhibit Moesin expression. I have had people advise me that my initial MOI should be low as 'less is more' and 'a little goes a long way' in terms of siRNA. I was wondering if you could elaborate on this for me and explain why my initial MOI should be low.
   • The reason for a low MOI is most likely because RNAi is a very strong and efficient technique. Wikipedia does a good job of explaining <a href="http://en.wikipedia.org/wiki/RNA_interference" target="_blank">RNA interference</a>. However, I would imagine that in a cell, there will be at most 1-2 copies of the gene mRNA present at any given time, unless you're dealing with a highly expressed protein such as Actin, where I would imagine silencing Actin would be lethal to the cell. I can imagine a few reasons to not use too much siRNA. First, it is expensive, so you don't want to waste it. Second, using too much would cause there to be a lot of non-translatable RNA present in the cell, which could trigger an immune response, as the presence of uncapped RNAs can indicate presence of a virus and one of the TLRs may respond to this.

Bioinformatics

• Gene Symbol: MSN
• Entrez:
  • 4478(Human)
  • 17698(Mouse)
  • 81521(Rat)
• Uniprot:
  • NP_002435.1(Human)