MMRN2 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

MMRN2 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human MMRN2.
  
  

  Source: E. coli

  Amino Acid Sequence: HAQHFTLHRSISELQADVDTKLKRLHKAQEAPGTNGSLVLATPGAGARPEPDSLQARLGQLQRNLSELHMTTARREEELQYTLEDMRATLTRHVDEIKELYSESDETFDQISKVERQVEELQVNHTALRELRVILMEK

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: MMRN2
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-83977.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for MMRN2 Recombinant Protein Antigen

• Elastin microfibril interface located protein 3
• Elastin microfibril interfacer 3EMILIN-3
• EMILIN3
• EMILIN-like protein EndoGlyx-1
• EndoGlyx-1 p125/p140 subunit
• Endoglyx-1
• FLJ13465
• MMRN2
• multimerin 2
• multimerin-2

Background

The MMRN2 gene encodes a 949 amino acid long, 104 kDA multimerin-2 protein that prevents endothelial cells motility. The elastic microfibril interface-located (EMILIN) protein family is an extracellular matrix glycoprotein. Additionally, this protein acts as a negative regulator of angiogenesis by downregulating activation by binding VEGFA. MMRN2 has been linked to adenocarcinoma and skin cancer as it participates in MAPK signaling, PTEN pathway, transendothelial migration of leukocytes, and UPA-UPAR pathway. It has been known to interact with genes VDR, MAPK7, MAP3K3, and DAXX.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MMRN2