MMP-9 Antibody

Western Blot: MMP-9 Antibody [NBP1-39597] - Analysis of anti-MMP-9 antibody with mouse liver tissue lysate.

Immunohistochemistry-Paraffin: MMP-9 Antibody [NBP1-39597] - Analysis of anti-MMP-9 antibody with human spleen, neutrophils at concentration 5 ug/ml.

Product Discontinued

MMP-9 Antibody Summary

• Immunogen: Synthetic peptide from human MMP9.
• Specificity: KLH conjugated synthetic peptide comprising amino acids 659 - 674 [LDTHDVFQYREKAYFC] of the human matrix metalloproteinase 9 (MMP9) protein.
• Predicted Species: Rabbit (93%), Primate (100%), Equine (93%), Monkey (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MMP9
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:100-1:500
• Control:

  Human Artery Membrane Tissue Lysate (Adult Membrane Normal)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gibbon (100%), Marmoset (100%), Elephant (93%), Canine (80%), Porcine (80%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for MMP-9 Antibody

• 92 kDa gelatinase
• 92 kDa type IV collagenase
• CLG4B
• EC 3.4.24
• EC 3.4.24.35
• Gelatinase B
• GELB
• macrophage gelatinase
• MANDP2
• matrix metallopeptidase 9
• matrix metalloproteinase 9
• matrix metalloproteinase-9
• MMP9
• MMP-9
• type V collagenase

Background

Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases that degrade substances within the extracellular matrix. The MMP family includes six different groups of enzymes: collagenases, gelatinases, stromelysins, transmembrane MMPs, matrilysins and others. MMPs are secreted as proenzymes that have to be cleaved in order to be activated. Other MMPs, plasmins as well as other factors activate MMPs. MMPs are thought to play an important role in tissue remodeling associated with various physiological and pathological processes. MMP9 degrades of proteins in the extracellular matrix and activates growth factors like proTGF beta and proTNF alpha. MMP9 contributes to the invasion and metastasis of various human malignancies. Clone F37P4A3 has been shown to be useful for western blotting, immunoflorence staining and immunohistochemistry of human, rabbit, dog, and pig acetylated MMP9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for MMP-9 Antibody (NBP1-39597). (Showing 1 - 1 of 1 FAQ).

1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
   • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.

Bioinformatics

• Gene Symbol: MMP9
• Entrez:
  • 4318(Human)
• Uniprot:
  • P14780()