MMP-9 Antibody (526515) - Pro form

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line stimulated with 100 ng/ml PMA for 24 hours under reducing (R) and non-reducing (NR) conditions. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human Pro MMP-9 Monoclonal Antibody (Catalog # MAB9111) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Pro MMP-9 was detected at approximately 85-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IP
• Clone: 526515
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MMP-9 Antibody (526515) - Pro form Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MMP‑9
  Ala20-Asp707 (Gln279Arg)
  Accession # P14780
• Specificity: Detects human Pro MMP-9 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human MMP-1, 2, 3, 7, 8, 10, 12, 13, 14, 16, or recombinant mouse MMP-9 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP9
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-9 Antibody (526515) - Pro form

• 92 kDa gelatinase
• 92 kDa type IV collagenase
• CLG4B
• EC 3.4.24
• EC 3.4.24.35
• Gelatinase B
• GELB
• macrophage gelatinase
• MANDP2
• matrix metallopeptidase 9
• matrix metalloproteinase 9
• matrix metalloproteinase-9
• MMP9
• MMP-9
• type V collagenase

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (Gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-9 Antibody (MAB9111)(3)

Publications using MAB9111

• Pambianchi E, Ferrara F, Pecorelli A et al. Deferoxamine Treatment Improves Antioxidant Cosmeceutical Formulation Protection against Cutaneous Diesel Engine Exhaust Exposure Antioxidants (Basel, Switzerland) 11/30/2021 [PMID: 34943031]
• Y Higashi, S Mummidi, S Sukhanov, T Yoshida, M Noda, P Delafontai, B Chandrasek Minocycline inhibits PDGF-BB-induced human aortic smooth muscle cell proliferation and migration by reversing miR-221- and -222-mediated RECK suppression Cell. Signal., 2019-02-01;57(0):20-Oct. 2019-02-01 [PMID: 30716386] (Western Blot, Human)
• Jon Christensen, V Prasad Shastri Matrix-metalloproteinase-9 is cleaved and activated by Cathepsin K BMC Research Notes 2015-12-01 [PMID: 26219353]

FAQs for MMP-9 Antibody (MAB9111). (Showing 1 - 1 of 1 FAQs).

1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
   • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.

Bioinformatics

• Gene Symbol: MMP9
• Uniprot:
  • P14780()