MMP-9 Antibody (116125) [Biotin] - Pro form

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA(Det)
Clone
116125
Clonality
Monoclonal
Host
Rat
Conjugate
Biotin
Concentration
LYOPH

Order Details

MMP-9 Antibody (116125) [Biotin] - Pro form Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MMP‑9
Ala20-Pro730
Accession # P41245.1
Specificity
Detects mouse Pro-MMP-9 in direct ELISAs. Does not detect the mature form of MMP-9. In sandwich ELISAs, no cross-reactivity was observed with recombinant human (rh) MMP-1, rhMMP-2, rhMMP-3, rhMMP-8, rhMMP-10, rhMMP-12, and rhMMP‑13.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
MMP9
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Mouse Pro-MMP-9 Antibody (Catalog # MAB9092)
ELISA Detection: Mouse Pro-MMP-9 Biotinylated Antibody (Catalog # BAM909)
Standard: Recombinant Mouse MMP-9 (Catalog # 909-MM)
Readout System
Publications
Read Publications using
BAM909 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-9 Antibody (116125) [Biotin] - Pro form

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 may be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain. Compared to the human MMP-9 (R&D Systems, Catalog # 911-MP), the mouse enzyme contains extra sequences in the linker region and in the hemopexin-like domain, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-9 Antibody (BAM909)(3)

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FAQs for MMP-9 Antibody (BAM909). (Showing 1 - 1 of 1 FAQs).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&applications=ELISA&common_name=MMP-9It looks like 2 have been tested for capture (please note the tested species for each of these).https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Capture%20(Matched%20Antibody%20Pair)1 has been tested for detection.https://www.novusbio.com/search?keywords=MMP-9&category=Primary%20Antibodies&common_name=MMP-9&applications=ELISA%20Detection%20(Matched%20Antibody%20Pair)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAM909

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Bioinformatics

Gene Symbol MMP9
Uniprot