MMP-9 Antibody (116125) [Biotin] - Pro form

• Reactivity: Mu
• Applications: ELISA(Det)
• Clone: 116125
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin
• Concentration: LYOPH

MMP-9 Antibody (116125) [Biotin] - Pro form Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MMP‑9
  Ala20-Pro730
  Accession # P41245.1
• Specificity: Detects mouse Pro-MMP-9 in direct ELISAs. Does not detect the mature form of MMP-9. In sandwich ELISAs, no cross-reactivity was observed with recombinant human (rh) MMP-1, rhMMP-2, rhMMP-3, rhMMP-8, rhMMP-10, rhMMP-12, and rhMMP‑13.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: MMP9
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Mouse Pro-MMP-9 Antibody (Catalog # MAB9092)
  ELISA Detection: Mouse Pro-MMP-9 Biotinylated Antibody (Catalog # BAM909)
  Standard: Recombinant Mouse MMP-9 (Catalog # 909-MM)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-9 Antibody (116125) [Biotin] - Pro form

• 92 kDa gelatinase
• 92 kDa type IV collagenase
• CLG4B
• EC 3.4.24
• EC 3.4.24.35
• Gelatinase B
• GELB
• macrophage gelatinase
• MANDP2
• matrix metallopeptidase 9
• matrix metalloproteinase 9
• matrix metalloproteinase-9
• MMP9
• MMP-9
• type V collagenase

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 may be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain. Compared to the human MMP-9 (R&D Systems, Catalog # 911-MP), the mouse enzyme contains extra sequences in the linker region and in the hemopexin-like domain, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-9 Antibody (BAM909)(3)

Publications using BAM909

• KG Blecharz-L, V Prinz, M Burek, D Frey, T Schenkel, SM Krug, M Fromm, P Vajkoczy Gelatinolytic activity of autocrine matrix metalloproteinase-9 leads to endothelial de-arrangement in Moyamoya disease J. Cereb. Blood Flow Metab., 2018-04-10;0(0):271678X187684. 2018-04-10 [PMID: 29633884] (Western Blot, Human)
• Miraglia M, Scian R, Samartino C, Barrionuevo P, Rodriguez A, Ibanez A, Coria L, Velasquez L, Baldi P, Cassataro J, Delpino M, Giambartolomei G Brucella abortus induces TNF-alpha-dependent astroglial MMP-9 secretion through mitogen-activated protein kinases. J Neuroinflammation, 2013-04-12;10(0):47. 2013-04-12 [PMID: 23587438] (ELISA Development (Detection), Mouse)
• Yurdakul P, Dalton J, Beattie L, Brown N, Erguven S, Maroof A, Kaye PM Compartment-specific remodeling of splenic micro-architecture during experimental visceral leishmaniasis. Am. J. Pathol., 2011-04-30;179(1):23-9. 2011-04-30 [PMID: 21703391] (Flow Cytometry, IHC-P, Mouse)

FAQs for MMP-9 Antibody (BAM909). (Showing 1 - 1 of 1 FAQs).

1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
   • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.

Bioinformatics

• Gene Symbol: MMP9
• Uniprot:
  • P41245.1()