Mouse MMP-7 ELISA Kit (Colorimetric)

ELISA: Mouse MMP-7 ELISA Kit (Colorimetric) [NBP3-06895] - Samples were spiked with high concentrations of Mouse MMP-7 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse MMP-7 ELISA Kit (Colorimetric) [NBP3-06895] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 62.5 - 4000 pg/mL
• Sensitivity: 37.5 pg/mL

Mouse MMP-7 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse MMP-7 in samples. No significant cross-reactivity or interference between Mouse MMP-7 and analogues was observed.
• Standard Curve Range: 62.5 - 4000 pg/mL
• Sensitivity: 37.5 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 5.17
• Intra-Assay: 5.33
• Spike Recovery: 86-101 %
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MMP7

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse MMP-7 ELISA Kit (Colorimetric)

• EC 3.4.24
• EC 3.4.24.23
• Matrilysin
• matrin
• matrix metallopeptidase 7 (matrilysin, uterine)
• matrix metalloproteinase 7 (matrilysin, uterine)
• Matrix metalloproteinase-7
• MMP7
• MMP-7
• MPSL1
• Pump-1 protease
• PUMP1
• PUMP-1
• uterine matrilysin
• Uterine metalloproteinase

Background

MMP7 is a Metallopeptidase M10A type protease. MMP-7 (Matrilysin) is constitutively produced by exocrine epithelial cells through out the body. It is capable of degrading casein, fibronectin, gelatins of types 1, III, IV, and V, in addition to activating procollagenase. It has been shown to play a key role in invasion by carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for MMP-7 ELISA Kit (NBP3-06895)(1)

Publication using NBP3-06895

• Annalisa Murgo, Fabio Bignami, Giuseppina Federico, Gino Villetti, Maurizio Civelli, Angelo Sala, Daniela Miglietta Harnessing the translational power of bleomycin model: new insights to guide drug discovery for idiopathic pulmonary fibrosis Frontiers in Pharmacology 2023-11-30 [PMID: 38099140]

FAQs for MMP-7 ELISA Kit (NBP3-06895). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: MMP7