MMP-7 Antibody (MM0022-4C21) - Azide and BSA Free

Immunohistochemistry-Paraffin: MMP-7 Antibody (MM0022-4C21) [NB110-60988] - Formalin fixed and Paraffin embedded human placenta tissue section was subjected to IHC staining using NB110-60988.

Western blot shows lysates of A549 human lung carcinoma parental cell line and MMP-7 knockout A549 cell line (KO). Nitrocellulose membrane was probed with MMP-7 Antibody (MM0022-4C21) - Azide and BSA Free (Catalog # NB110-60988) followed by HRP-conjugated secondary antibody. A specific band was detected for MMP-7 at approximately 29.7 kDa (as indicated) in the parental A549 cell line, but is not detectable in knockout A549 cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Product Discontinued

MMP-7 Antibody (MM0022-4C21) - Azide and BSA Free Summary

• Immunogen: Human recombinant MMP-7
• Specificity: This antibody recognizes human MMP-7 in western blot without cross reactivity with other human MMPs. Recognizes both pro- (28 kDa) and active-form (18 kDa).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for MMP-7 Antibody (MM0022-4C21) - Azide and BSA Free

• EC 3.4.24
• EC 3.4.24.23
• Matrilysin
• matrin
• matrix metallopeptidase 7 (matrilysin, uterine)
• matrix metalloproteinase 7 (matrilysin, uterine)
• Matrix metalloproteinase-7
• MMP7
• MMP-7
• MPSL1
• Pump-1 protease
• PUMP1
• PUMP-1
• uterine matrilysin
• Uterine metalloproteinase

Background

Matrix Metalloproteinases (MMPs) belong to a family of proteases that are essential for the breakdown of extracellular matrix (ECM). They are thus important in apoptosis, tumor cell growth, invasion, and metastasis, as well as angiogenesis and wound healing. Most MMPs contain common domain structures that include a signal sequence, a propeptide, a catalytic domain, and a hemopexin like (Hpx) domain. MMP7, also known as Matrilysin, is responsible for hydrolyzing proteoglycans and ECM glycoproteins. It is expressed in normal cells, specifically glandular epithelial cells. MMP7, is thought to play an important role in the early steps of tumor progression. Expression of MMP2, MMP7 and MMP11 is greater in pancreatic carcinoma than in normal pancreas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-7 Antibody (NB110-60988)(3)

Publications using NB110-60988

• Boukhedouni N, Martins C, Darrigade AS et al. Type-1 cytokines regulate MMP-9 production and E-cadherin disruption to promote melanocyte loss in vitiligo JCI Insight 2020-06-04 [PMID: 32369451] (IHC-P, Human)
• Singh M, Kindelberger D, Nagymanyoki Z et al. Matrix metalloproteinases and their inhibitors and inducer in gestational trophoblastic diseases and normal placenta Gynecol Oncol 2011-07-01 [PMID: 21514631] (IF/IHC, Human)
• Davidson B, Stavnes HT, Holth A et al. Gene expression signatures differentiate ovarian/peritoneal serous carcinoma from breast carcinoma in effusions. J Cell Mol Med 2011-03-01 [PMID: 20132413] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: MMP7
• Entrez:
  • 4316(Human)
• Uniprot:
  • P09237()