Human MMP-25/MT6-MMP ELISA Kit (Colorimetric)

ELISA: Human MMP-25/MT6-MMP ELISA Kit (Colorimetric) [NBP3-06943] - Samples were spiked with high concentrations of Human MMP-25/MT6-MMP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human MMP-25/MT6-MMP ELISA Kit (Colorimetric) [NBP3-06943] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL

Human MMP-25/MT6-MMP ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human MMP-25/MT6-MMP in samples. No significant cross-reactivity or interference between Human MMP-25/MT6-MMP and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 5.15
• Intra-Assay: 4.52
• Spike Recovery: 88-102 %
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MMP25

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human MMP-25/MT6-MMP ELISA Kit (Colorimetric)

• Leukolysin
• matrix metallopeptidase 25
• matrix metallopeptidase-like 1
• matrix metalloproteinase 20
• matrix metalloproteinase 25
• matrix metalloproteinase-25
• matrix metalloproteinase-like 1
• membrane-type 6 matrix metalloproteinase
• membrane-type matrix metalloproteinase 6
• membrane-type-6 matrix metalloproteinase
• MMP20
• MMP20A
• MMP25
• MMP-25
• MMPL1
• MT6MMP
• MT6-MMP
• MT-MMP 6
• MTMMP6
• MT-MMP6

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling. They are also involved in disease processes, such as metastasis and arthritis. Most MMPs are secreted as inactive proproteins which are then activated when cleaved by extracellular proteinases. The protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily, and is attached to the plasma membrane via a glycosylphosphatidyl inositol anchor. The encoded protein is thought to inactivate alpha-1 proteinase inhibitor, a major tissue protectant against proteolytic enzymes released by activated neutrophils, facilitating the transendothelial migration of neutrophils to inflammatory sites in response to bacterial infection or inflammation. The encoded protein may also play a role in tumor invasion and metastasis through activation of MMP2. The gene has previously been referred to as MMP20 but has been renamed MMP25.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for MMP-25/MT6-MMP ELISA Kit (NBP3-06943). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: MMP25