MMP-19 Overexpression Lysate (Native)

Images

 
Western Blot: MMP19 Overexpression Lysate (Adult Normal) [NBL1-13155] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MMP19.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MMP-19 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC208245 and based on accession number NM_002429. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens matrix metallopeptidase 19 (MMP19), transcript variant 1, mRNA.
Gene
MMP19

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
55.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MMP-19

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MMP-19 Overexpression Lysate (Native)

  • EC 3.4.24.-
  • matrix metallopeptidase 19
  • matrix metalloproteinase 18
  • matrix metalloproteinase 19
  • Matrix metalloproteinase RASI
  • Matrix metalloproteinase-18
  • matrix metalloproteinase-19
  • MMP-18
  • MMP18MMP-19
  • MMP19
  • MMP-19
  • RASI
  • RASI-1

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This protein is expressed in human epidermis and it has a role in cellular proliferation as well as migration and adhesion to type I collagen. Multiple transcript variants encoding distict isoforms have been identified for this gene. Transcript Variant: This variant 1, alternatively referred to as rasi-1, represents the longer transcript and encodes the longer isoform (rasi-1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for MMP-19 Lysate (NBL1-13155) (0)

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Bioinformatics

Gene Symbol MMP19
Entrez