Recombinant Human MMP-14/MT1-MMP Protein

SDS-Page: Recombinant Human MMP-14/MT1-MMP Protein [NBP2-61379] - SDS-Page of Recombinant Human MMP-14/MT1-MMP Protein under reducing and non-reducing conditions

• Reactivity: Hu
• Applications: PAGE

Recombinant Human MMP-14/MT1-MMP Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 264 amino acids corresponding to MMP-14/MT1-MMP Source: E. coli

  Uniprot ID: P50281

  Amino Acid Sequence: ALASLGSAQS SSFSPEAWLQ QYGYLPPGDL RTHTQRSPQS LSAAIAAMQK FYGLQVTGKA DADTMKAMRR PRCGVPDKFG AEIKANVRRK RYAIQGLKWQ HNEITFCIQN YTPKVGEYAT YEAIRKAFRV WESATPLRFR EVPYAYIREG HEKQADIMIF FAEGFHGDST PFDGEGGFLA HAYFPGPNIG GDTHFDSAEP WTVRNEDLNG NDIFLVAVHE LGHALGLEHS SDPSAIMAPF YQWMDTENFV LPDDDRRGIQ QLYG

• Details of Functionality: Bioassay data are not available
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MMP14
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of MMP-14/MT1-MMP as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 29.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in 20 mM Tris-HCl, pH 7.4, 300 mM NaCl, 3 mM CaCl2, 10 uM ZnCl2
• Preservative: 30% glycerol
• Purity: >95%, by SDS-PAGE and HPLC

Alternate Names for Recombinant Human MMP-14/MT1-MMP Protein

• EC 3.4.24
• EC 3.4.24.80
• matrix metallopeptidase 14 (membrane-inserted)
• matrix metalloproteinase 14 (membrane-inserted)
• matrix metalloproteinase-14
• membrane type 1 metalloprotease
• Membrane-type matrix metalloproteinase 1
• Membrane-type-1 matrix metalloproteinase
• MMP14
• MMP-14
• MMP-X1
• MT1MMP
• MT1-MMP
• MT1-MMPMTMMP1
• MT-MMP 1
• MT-MMP1

Background

The matrix metalloproteinase (MMP) family (approximately 25 members in mammals) has been implicated in extracellular matrix remodeling associated with embryonic development, cancer formation and progression, and various other physiological and pathological events. MMP-14 is a membrane-bound matrix metalloproteinase (MT-MMP) capable of mediating pericellular proteolysis of extracellular matrix components. MMP-14 is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix (1). Gelatinase A (type-IV collagenase; M(r) 72,000) is produced by tumour stroma cells and is believed to be crucial for their invasion and metastasis, acting by degrading extracellular matrix macro-molecules such as type IV collagen. MMP-14 may thus trigger invasion by tumor cells by activating pro-gelatinase A on the tumor cell surface (2). Observations identify MT1-MMP as a tumor-derived growth factor that regulates proliferation by controlling cell geometry within the confines of the 3D extracellular matrix (ECM) (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MMP14