MMP-13 Antibody [Unconjugated]

Images

 
MMP‑13 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Goat Anti-HumanMMP‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF511) overnight at 4 ...read more
MMP‑13 was detected in immersion fixed paraffin-embedded sections of human breast array using Goat Anti-Human MMP‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF511) at 15 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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MMP-13 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP-13
Leu20-Cys471
Accession # P45452
Specificity
Detects human MMP-13 in Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) MMP-3 and rhMMP-8 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MMP13
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF511 in the following applications:

Publications
Read Publications using
AF511 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-13 Antibody [Unconjugated]

  • CLG 3
  • CLG3EC 3.4.24
  • collagenase 3
  • EC 3.4.24.-
  • EC 3.4.24.22
  • EC 3.4.24.24
  • EC 3.4.24.35
  • EC 3.4.24.65
  • EC 3.4.24.7
  • MANDP1
  • matrix metallopeptidase 13 (collagenase 3)
  • matrix metalloproteinase 13 (collagenase 3)
  • Matrix metalloproteinase-13
  • MMP13
  • MMP-13

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is distinguished from the other human collagenases by its effecient degradation of type II collagen. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. Structurally, MMP-13 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

  1. Jeffery, J.J. (1998) in Collagenase 3. A.J. Barrett, et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1167.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-13 Antibody (AF511)(4)

We have publications tested in 2 confirmed species: Human, Rabbit.

We have publications tested in 2 applications: ELISA Development, Western Blot.


Filter By Application
ELISA Development
(1)
Western Blot
(3)
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Filter By Species
Human
(3)
Rabbit
(1)
All Species

Review for MMP-13 Antibody (AF511) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF511:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
WB Human 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22521401
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22521401

Details

Detection NotesSee PMID 22521401

Comments

CommentsPublished: See PMID 22521401Independent review from antibodyresource.comgood sensitivity but tricky reproducibility from sample to sample. use alioquots quickly aftyer thawing.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF511

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Bioinformatics Tool for MMP-13 Antibody (AF511)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Anonymous
01/08/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol MMP13
Uniprot