MMP-13 Antibody (87512) [Unconjugated]

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MMP‑13 was detected in immersion fixed MDA‑MB‑231 human breast cancer cell line using Human MMP‑13 Monoclonal Antibody (Catalog # MAB511) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more
MMP‑13 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 8 µg/mL Mouse Anti-Human MMP‑13 Monoclonal Antibody (Catalog # MAB511) overnight at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, AP, ICC/IF
Clone
87512
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

MMP-13 Antibody (87512) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-13
Leu20-Cys471
Accession # P45452
Specificity
Detects human MMP-13 in direct ELISAs and Western blots. Detects both pro and active forms of human MMP-13 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, or -12 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MMP13
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Immunoaffinity Purification
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
MAB511 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-13 Antibody (87512) [Unconjugated]

  • CLG 3
  • CLG3EC 3.4.24
  • collagenase 3
  • EC 3.4.24.-
  • EC 3.4.24.22
  • EC 3.4.24.24
  • EC 3.4.24.35
  • EC 3.4.24.65
  • EC 3.4.24.7
  • MANDP1
  • matrix metallopeptidase 13 (collagenase 3)
  • matrix metalloproteinase 13 (collagenase 3)
  • Matrix metalloproteinase-13
  • MMP13
  • MMP-13

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is distinguished from the other human collagenases by its effecient degradation of type II collagen. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. Structurally, MMP-13 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

  1. Jeffery, J.J. (1998) in Collagenase 3. A.J. Barrett, et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1167.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
Applications: WB, IHC, IP, AP, ICC/IF

Publications for MMP-13 Antibody (MAB511)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 6 applications: ELISA Development, ICC, IHC Frozen, IHC Paraffin-embedded, Neut, WB.


Filter By Application
ELISA Development
(1)
ICC
(1)
IHC Frozen
(1)
IHC Paraffin-embedded
(1)
Neut
(1)
WB
(3)
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Filter By Species
Human
(6)
Mouse
(1)
Canine
(1)
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Showing Publications 1 - 8 of 8.
Publications using MAB511 Applications Species
Orgaz J, Pandya P, Dalmeida R, Karagiannis P, Sanchez-Laorden B, Viros A, Albrengues J, Nestle F, Ridley A, Gaggioli C, Marais R, Karagiannis S, Sanz-Moreno V Diverse matrix metalloproteinase functions regulate cancer amoeboid migration. Nat Commun, 2014;5(0):4255. 2014 [PMID: 24963846] (WB,, Human) WB Human
Nurmenniemi S, Kuvaja P, Lehtonen S Toll-like receptor 9 ligands enhance mesenchymal stem cell invasion and expression of matrix metalloprotease-13. Exp. Cell Res., 2010;316(16):2676-82. 2010 [PMID: 20553713] (Neut,, Human) Neut Human
Cavallo C, Desando G, Facchini A, Grigolo B Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold. J Biomed Mater Res A, 2010;93(1):86-95. 2010 [PMID: 19484766] (ICC,, Human) ICC Human
Ohlund D, Ardnor B, Oman M, Naredi P, Sund M Expression pattern and circulating levels of endostatin in patients with pancreas cancer. Int. J. Cancer, 2008;122(12):2805-10. 2008 [PMID: 18360823] (IHC Frozen,, Human) IHC Frozen Human
Peng Z, Fernandez P, Wilder T, Yee H, Chiriboga L, Chan ES, Cronstein BN Ecto-5'-nucleotidase (CD73) -mediated extracellular adenosine production plays a critical role in hepatic fibrosis. FASEB J., 2008;22(7):2263-72. 2008 [PMID: 18263696] (WB,, Mouse) WB Mouse
Im HJ, Muddasani P, Natarajan V, Schmid TM, Block JA, Davis F, van Wijnen AJ, Loeser RF Basic fibroblast growth factor stimulates matrix metalloproteinase-13 via the molecular cross-talk between the mitogen-activated protein kinases and protein kinase Cdelta pathways in human adult articular chondrocytes. J. Biol. Chem., 2007;282(15):11110-21. 2007 [PMID: 17311929] (WB,, Human) WB Human
Olivotto E, Vitellozzi R, Fernandez P, Falcieri E, Battistelli M, Burattini S, Flamigni F, Santi S, Borzi RM Chondrocyte hypertrophy and apoptosis induced by GROalpha require three-dimensional interaction with the extracellular matrix and a co-receptor role of chondroitin sulfate and are associated with the mitochondrial splicing variant of cathepsin B. J. Cell. Physiol., 2007;210(2):417-27. 2007 [PMID: 17096385] (ELISA Development,, Human) ELISA Development Human
Pelletier JP, Boileau C, Brunet J, Boily M, Lajeunesse D, Reboul P, Laufer S, Martel-Pelletier J The inhibition of subchondral bone resorption in the early phase of experimental dog osteoarthritis by licofelone is associated with a reduction in the synthesis of MMP-13 and cathepsin K. Bone, 2004;34(3):527-38. 2004 [PMID: 15003800] (IHC Paraffin-embedded,, Canine) IHC Paraffin-embedded Canine

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Bioinformatics

Gene Symbol MMP13
Uniprot