MMP-13 Antibody (87512) [Janelia Fluor® 525]

• Reactivity: Hu
• Applications: WB, IHC, IP, AP, ICC/IF
• Clone: 87512
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 525

MMP-13 Antibody (87512) [Janelia Fluor® 525] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human MMP-13
  Leu20-Cys471
  Accession # P45452
• Specificity: Detects human MMP-13 in direct ELISAs and Western blots. Detects both pro and active forms of human MMP-13 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, or -12 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP13
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoaffinity Purification
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for MMP-13 Antibody (87512) [Janelia Fluor® 525]

• CLG 3
• CLG3EC 3.4.24
• collagenase 3
• EC 3.4.24.-
• EC 3.4.24.22
• EC 3.4.24.24
• EC 3.4.24.35
• EC 3.4.24.65
• EC 3.4.24.7
• MANDP1
• matrix metallopeptidase 13 (collagenase 3)
• matrix metalloproteinase 13 (collagenase 3)
• Matrix metalloproteinase-13
• MMP13
• MMP-13

Background

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 (also designated macrophage metalloelastase) is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration through the epidermal and vascular basement membranes in inflammatory disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MMP13