MMP-11 Antibody (135421)

• Reactivity: Hu
• Applications: CyTOF-ready, Flow
• Clone: 135421
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MMP-11 Antibody (135421) Summary

• Immunogen: E. coli-derived recombinant human MMP‑11
  Met1-Leu488
  Accession # P24347
• Specificity: Detects human MMP-11 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP11
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-11 Antibody (135421)

• EC 3.4.24
• EC 3.4.24.-
• EC 3.4.24.24
• matrix metallopeptidase 11 (stromelysin 3)
• matrix metalloproteinase 11 (stromelysin 3)
• Matrix metalloproteinase-11
• MMP11
• MMP-11
• SL-3
• ST3
• STMY3stromelysin 3
• stromelysin III
• stromelysin-3

Background

MMP-11, also called Stromelysin-3, is a metalloproteinase secreted by cells of mesenchymal origin. Human MMP-11 contains a 31 amino acid (aa) signal sequence, a 65 aa propeptide cleaved at a furin consensus sequence, and a 390 aa mature sequence with zinc metalloproteinase and hemopexin-like domains. The human 45 kDa mature MMP-11 shows 89% aa identity with mouse. An inducible low-abundance 40 kDa isoform is a constitutively active intracellular form. MMP-11 is upregulated in stromal cells near many invasive carcinomas, where it can cleave IGFBP, allowing IGF-1 to promote tumor cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-11 Antibody (MAB3657)(1)

Publication using MAB3657

• V Borgna, L Lobos-Gonz, F Guevara, E Landerer, M Bendek, R Ávila, V Silva, C Villota, M Araya, A Rivas, C López, T Socias, J Castillo, L Alarcón, LO Burzio, VA Burzio, J Villegas Targeting antisense mitochondrial noncoding RNAs induces bladder cancer cell death and inhibition of tumor growth through reduction of survival and invasion factors J Cancer, 2020-01-17;11(7):1780-1791. 2020-01-17 [PMID: 32194789] (Western Blot, Human)

Bioinformatics

• Gene Symbol: MMP11
• Uniprot:
  • P24347()