![Western Blot: MLKL Antibody [H00197259-B01P] - Analysis of MLKL expression in transfected 293T cell line by MLKL polyclonal antibody. Lane 1: MLKL transfected lysate(51.81 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MLKL-Antibody-Western-Blot-H00197259-B01P-img0004.jpg "Western Blot: MLKL Antibody [H00197259-B01P] - Analysis of MLKL expression in transfected 293T cell line by MLKL polyclonal antibody. Lane 1: MLKL transfected lysate(51.81 KDa). Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | MLKL (NP_689862.1, 1 a.a. - 471 a.a.) full-length human protein. MENLKHIITLGQVIHKRCEEMKYCKKQCRRLGHRVLGLIKPLEMLQDQGKRSVPSEKLTTAMNRFKAALEEANGEIEKFSNRSNICRFLTASQDKILFKDVNRKLSDVWKELSLLLQVEQRMPVSPISQGASWAQEDQQDADEDRRAFQMLRRDNEKIEASLRRLEINMKEIKETLRQYLPPKCMQEIPQEQIKEIKKEQLSGSPWILLRENEVSTLYKGEYHRAPVAIKVFKKLQAGSIAIVRQTFNKEIKTMKKFESPNILRIFGICIDETVTPPQFSIVMEYCELGTLRELLDREKDLTLGKRMVLVLGAARGLYRLHHSEAPELHGKIRSSNFLVTQGYQVKLAGFELRKTQTSMSLGTTREKTDRVKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK |
| Specificity | MLKL - mixed lineage kinase domain-like, |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Mouse |
| Gene | MLKL |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against Recombinant Protein with GST tag on Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. It has also been used for immunofluorescence. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for MLKL Antibody (H00197259-B01P)Find related products by research area.
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Rest in Peace: Is the Receptor Interacting Protein (RIP) Kinase-3 (RIPK3) a Protector or Offender? By Jamshed Arslan, Pharm. D., PhD. Receptor interacting protein kinases (RIPKs) in necroptosisDeath is perhaps inevitable. Cell death can be programed and immunologically silent (apoptosis), unprogrammed and inflamm... Read full blog post. |
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Apoptosis and Necroptosis Part II: Inhibitors of apoptosis proteins (IAPs); Key regulators of the balance between necroptosis, apoptosis and survival In the first installment of this two-part blog post titled "Apoptosis and Necroptosis: Important factors to identify both types of programmed cell death", the mechanisms by which cell death occurs and ways to identify these pathways were ... Read full blog post. |
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Apoptosis and Necroptosis Part I: Important factors to identify both types of programmed cell death Different types of cell death have classically been identified by discrete morphological changes. The hallmarks of apoptosis include cell shrinkage, nuclear fragmentation and membrane blebbing whereas necroptosis is characterized by cell swelling ... Read full blog post. |
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| Gene Symbol | MLKL |
| Entrez |
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| Uniprot |
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![Immunocytochemistry/Immunofluorescence: MLKL Antibody [H00197259-B01P] - Analysis of purified antibody to MLKL on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/MLKL-Antibody-Immunocytochemistry-Immunofluorescence-H00197259-B01P-img0003.jpg "Immunocytochemistry/Immunofluorescence: MLKL Antibody [H00197259-B01P] - Analysis of purified antibody to MLKL on HeLa cell. (antibody concentration 10 ug/ml)")
![Western Blot: MLKL Antibody [H00197259-B01P] - Analysis of MLKL expression in transfected 293T cell line by MLKL MaxPab polyclonal antibody. Lane 1: MLKL transfected lysate (51.81 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MLKL-Antibody-Western-Blot-H00197259-B01P-img0001.jpg "Western Blot: MLKL Antibody [H00197259-B01P] - Analysis of MLKL expression in transfected 293T cell line by MLKL MaxPab polyclonal antibody. Lane 1: MLKL transfected lysate (51.81 KDa). Lane 2: Non-transfected lysate.")
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