Immunohistochemistry-Paraffin: MLKL [p Thr357] Antibody (954724) [MAB91871] - MLKL was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using MLKL Monoclonal Antibody at 5 ug/ml. ...read more
Immunohistochemistry: MLKL [p Thr357] Antibody (954724) [MAB91871] - MLKL was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using MLKL Monoclonal Antibody at 5 ug/ml. Tissue was ...read more
FAQs for MLKL Antibody (MAB91871). (Showing 1 - 1 of 1 FAQ).
hello! we're working with MAB91871. the IF staining is looking pretty good, but occasionally we are finding bright specular artifacts. From the secondary controls we have narrow it down to being due to the presence of the primary - do y'all have any handling data on this product and how to minimize aggregates?
Here are some general suggestions to try and reduce aggregation background signal in your organoid IF staining with MAB91871. This is a purified antibody with no BSA, so it ought not to be an issue with buffer but its possible it's could be sticky.Do you use 0.05-0.1 % Triton X 100?You might try adding NaCl to the blocking buffer/antibody diluent so that the final concentration is between 0.15 M and 0.6 M NaCl. The best NaCl concentration to use will have to be determined empirically, but this should help reduce ionic interactions and keep the AB soluble. If it's a blocking issue, you could of course increase the blocking buffer composition and/or concentration.It's possible that high primary AB concentration is leading to non-specific binding and background staining, so you might try reducing the final concentration of the primary antibody used.
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