MLKL [p Thr357] Antibody (954724)

Images

 
Immunohistochemistry-Paraffin: MLKL [p Thr357] Antibody (954724) [MAB91871] - MLKL was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using MLKL Monoclonal Antibody at 5 ug/ml. ...read more
Immunohistochemistry: MLKL [p Thr357] Antibody (954724) [MAB91871] - MLKL was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using MLKL Monoclonal Antibody at 5 ug/ml. Tissue was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P, Flow (-), ICC/IF
Clone
954724
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MLKL [p Thr357] Antibody (954724) Summary

Immunogen
Synthetic peptide from human MLKL phosphorylated at T357 [UniProt Q8NB16]
Modification
p Thr357
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MLKL
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Simple Western
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunocytochemistry
Application Notes
This MLKL [p Thr357] Antibody (954724) is validated for Simple Western from a verified customer review.
Reviewed Applications
Read 2 Reviews rated 4.5
using
MAB91871 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Notes

>90% by SDS PAGE

Alternate Names for MLKL [p Thr357] Antibody (954724)

  • FLJ34389
  • mixed lineage kinase domain-like protein
  • mixed lineage kinase domain-like
  • MLKL

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB91871
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Applications: WB, Simple Western, IHC, IHC-P, Flow (-), ICC/IF

Publications for MLKL Antibody (MAB91871) (0)

There are no publications for MLKL Antibody (MAB91871).
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Reviews for MLKL Antibody (MAB91871) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Human and Mouse.

Reviews using MAB91871:
Filter by Applications
Simple Western
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Human and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western MLKL MAB91871
Enlarge
5
reviewed by:
Anonymous
Simple Western Human and Mouse 10/16/2020
View

Summary

ApplicationSimple Western
Sample TestedHT-29 human colon adenocarcinoma cell line,MC-38
SpeciesHuman and Mouse
LotCKLX01-4

Comments

Comments*Novus Innovators Program - new species or application used on a primary antibody.
Immunohistochemistry-Paraffin MLKL MAB91871
Enlarge
4
reviewed by:
Taotao Zhang
IHC-P Human 10/09/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedFFPE
SpeciesHuman
LotCKLX01-4

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MLKL Antibody (MAB91871). (Showing 1 - 1 of 1 FAQ).

  1. hello! we're working with MAB91871. the IF staining is looking pretty good, but occasionally we are finding bright specular artifacts. From the secondary controls we have narrow it down to being due to the presence of the primary - do y'all have any handling data on this product and how to minimize aggregates?
    • Here are some general suggestions to try and reduce aggregation background signal in your organoid IF staining with MAB91871. This is a purified antibody with no BSA, so it ought not to be an issue with buffer but its possible it's could be sticky.Do you use  0.05-0.1 % Triton X 100?You might try adding NaCl to the blocking buffer/antibody diluent so that the final concentration is between 0.15 M and 0.6 M NaCl. The best NaCl concentration to use will have to be determined empirically, but this should help reduce ionic interactions and keep the AB soluble. If it's a blocking issue, you could of course increase the blocking buffer composition and/or concentration.It's possible that high primary AB concentration is leading to non-specific binding and background staining, so you might try reducing the final concentration of the primary antibody used.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 MAB91871AF350
Alexa Fluor 405 MAB91871AF405
Alexa Fluor 488 MAB91871AF488
Alexa Fluor 532 MAB91871AF532
Alexa Fluor 594 MAB91871AF594
Alexa Fluor 647 MAB91871AF647
Alexa Fluor 700 MAB91871AF700
Alexa Fluor 750 MAB91871AF750
Biotin MAB91871B
DyLight 350 MAB91871UV
DyLight 405 MAB91871V
DyLight 488 MAB91871G
DyLight 550 MAB91871R
DyLight 594 MAB91871DL594
DyLight 650 MAB91871C
DyLight 680 MAB91871FR
DyLight 755 MAB91871IR
FITC MAB91871F
HRP MAB91871H
Janelia Fluor 549 MAB91871JF549
Janelia Fluor 646 MAB91871JF646

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Bioinformatics Tool for MLKL Antibody (MAB91871)

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Research Areas for MLKL Antibody (MAB91871)

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Blogs on MLKL.

Rest in Peace: Is the Receptor Interacting Protein (RIP) Kinase-3 (RIPK3) a Protector or Offender?
By Jamshed Arslan, Pharm. D., PhD. Receptor interacting protein kinases (RIPKs) in necroptosisDeath is perhaps inevitable. Cell death can be programed and immunologically silent (apoptosis), unprogrammed and inflamm...  Read full blog post.

Apoptosis and Necroptosis Part II: Inhibitors of apoptosis proteins (IAPs); Key regulators of the balance between necroptosis, apoptosis and survival
In the first installment of this two-part blog post titled "Apoptosis and Necroptosis: Important factors to identify both types of programmed cell death", the mechanisms by which cell death occurs and ways to identify these pathways were...  Read full blog post.

Apoptosis and Necroptosis Part I: Important factors to identify both types of programmed cell death
Different types of cell death have classically been identified by discrete morphological changes. The hallmarks of apoptosis include cell shrinkage, nuclear fragmentation and membrane blebbing whereas necroptosis is characterized by cell swelling...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anonymous
10/16/2020
Application: Simple Western
Species: Human and Mouse

Taotao Zhang
10/09/2020
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol MLKL