MKRN2 Antibody - BSA Free

Western Blot: MKRN2 Antibody [NB100-55250] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. NB100-55250 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B), and used for IP at 3 mcg/mg lysate (B).

Immunohistochemistry-Paraffin: MKRN2 Antibody [NB100-55250] - Human breast carcinoma. Antibody: Affinity purified rabbit anti-MKRN2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MKRN2 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 366 and the C-terminus (residue 416) of human makorin, ring finger protein, 2 using the numbering given in entry NP_054879.3 (GeneID 23609).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MKRN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 to 1:5000
  • Immunohistochemistry-Paraffin 1:1000 to 1:5000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Control:

  MKRN2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MKRN2 Antibody - BSA Free

• EC 6.3.2.-
• HSPC070
• makorin ring finger protein 2
• probable E3 ubiquitin-protein ligase makorin-2
• RNF62RING finger protein 62

Background

The basic function of MKRN2 is mediating ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. Makorin RING zinc finger-2 (MKRN2), overlaps and is antisense to the gene RAF1 in mammals. Northern blot analyses show that human MKRN2 and RAF1 are co-expressed in tissues and cell lines, raising the possibility of mRNA duplex formation. The encoded makorin-2 protein is likely a ribonucleoprotein of unknown function, but its conservation suggests an important cellular role.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MKRN2 Antibody (NB100-55250)(1)

Publication using NB100-55250

• Qian YC, Xie YX, Wang CS et al. Mkrn2 deficiency induces teratozoospermia and male infertility through p53/PERP-mediated apoptosis in testis Asian Journal of Andrology 2020-01-01 [PMID: 31489847]

Bioinformatics

• Gene Symbol: MKRN2
• Entrez:
  • 23609(Human)
  • 67027(Mouse)
• Uniprot:
  • Q9H000(Human)