MKRN2 Overexpression Lysate

Images

 
Western Blot: MKRN2 Overexpression Lysate (Adult Normal) [NBL1-13127] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MKRN2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MKRN2 Overexpression Lysate Summary

Description

MKRN2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC203714

Accession#: NM_014160

Protein Tag: C-MYC/DDK
Gene
MKRN2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
46.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MKRN2 Overexpression Lysate

  • EC 6.3.2.-
  • HSPC070
  • makorin ring finger protein 2
  • probable E3 ubiquitin-protein ligase makorin-2
  • RNF62RING finger protein 62

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-45816
Species: Hu, Mu
Applications: Flow, IHC, IHC-P, WB
MAB4540
Species: Hu, Mu, Rt
Applications: IHC, Simple Western, WB
NBP2-38464
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBP1-82956
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
PP-H3210-00
Species: Hu
Applications: DirELISA, IP, WB
NBP1-81838
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP2-13440
Species: Hu
Applications: IHC, IHC-P, WB
AF2209
Species: Hu
Applications: ELISA, IHC, WB
NBP2-19804
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-33116
Species: Bv, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-14081
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-85768
Species: Hu
Applications: IHC, IHC-P
NBP1-81013
Species: Hu
Applications: IHC, IHC-P
NBP2-15071
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
AF4096
Species: Hu
Applications: CyTOF-ready, Flow, ICC, WB
NB600-1071
Species: Hu(-), Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-46404
Species: Hu, Mu, Rt
Applications: IHC, IHC-P
AF761
Species: Mu
Applications: AdBlk, CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, Simple Western, WB
NBL1-13127
Species: Hu
Applications: WB

Publications for MKRN2 Lysate (NBL1-13127) (0)

There are no publications for MKRN2 Lysate (NBL1-13127).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MKRN2 Lysate (NBL1-13127) (0)

There are no reviews for MKRN2 Lysate (NBL1-13127). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MKRN2 Lysate (NBL1-13127) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional MKRN2 Products

Bioinformatics Tool for MKRN2 Lysate (NBL1-13127)

Discover related pathways, diseases and genes to MKRN2 Lysate (NBL1-13127). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MKRN2 Lysate (NBL1-13127)

Discover more about diseases related to MKRN2 Lysate (NBL1-13127).
 

Pathways for MKRN2 Lysate (NBL1-13127)

View related products by pathway.

Research Areas for MKRN2 Lysate (NBL1-13127)

Find related products by research area.

Blogs on MKRN2

There are no specific blogs for MKRN2, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MKRN2 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol MKRN2