MKP-3/DUSP6 Antibody (363246)

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and CTLL‑2 mouse cytotoxic T cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
363246
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

MKP-3/DUSP6 Antibody (363246) Summary

Immunogen
E. coli-derived recombinant human MKP-3
Ile2-Thr381
Accession # Q16828
Specificity
Detects endogenous human, mouse, and rat MKP-3 in Western blots. In Western blots, this antibody does not cross-react with recombinant human MKP-1 or MKP-2.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
DUSP6
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Control
MKP-3/DUSP6 Knockout HeLa Cell Lysate
Publications
Read Publication using
MAB3576 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MKP-3/DUSP6 Antibody (363246)

  • dual specificity phosphatase 6
  • Dual specificity protein phosphatase PYST1
  • DUSP6
  • EC 3.1.3.16
  • EC 3.1.3.48
  • MAP kinase phosphatase 3
  • Mitogen-activated protein kinase phosphatase 3
  • MKP3
  • MKP-3
  • MKP3serine/threonine specific protein phosphatase
  • PYST1
  • PYST1dual specificity protein phosphatase 6

Background

MAP Kinase Phosphatase 3 (MKP-3), also called PYST1 and Dual Specificity Phosphatase 6 (DUSP6), dephosphorylates serine, threonine, and tyrosine residues in proteins. On Western blots, MKP-3 has an apparent molecular weight of 42 kilodaltons. It has a high degree of substrate selectivity for dephosphorylating ERK kinases over stress-related kinases such as p38 and JNK. MKP-3 is ubiquitinylated prior to degradation in the proteosome. The rate of MKP-3 degradation is enhanced by ERK-mediated phosphorylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MKP-3/DUSP6 Antibody (MAB3576)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol DUSP6
Uniprot