CTS013). Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="MKP-1 was detected in immersion fixed paraffin-embedded sections of human prostate using Rat Anti-Human MKP-1 Monoclonal Antibody (Catalog # MAB7168) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7168-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57354-25ul | |||
![Western Blot: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of MKP-1/DUSP1 on SK-BR-3 cell tissue lysates with Rabbit anti-MKP-1/DUSP1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size:39 kDa Observed band size: 39 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-JJ0930-Western-Blot-NBP2-67909-img0005.jpg "Western Blot: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of MKP-1/DUSP1 on SK-BR-3 cell tissue lysates with Rabbit anti-MKP-1/DUSP1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size:39 kDa Observed band size: 39 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of paraffin-embedded mouse lung tissue using anti-DUSP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-JJ0930-Immunohistochemistry-Paraffin-NBP2-67909-img0003.jpg)
![Flow Cytometry: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of HepG2 cells with DUSP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-JJ0930-Flow-Cytometry-NBP2-67909-img0001.jpg)
![Western Blot: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of DUSP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysateLane 2: Jurkat cell lysate Lane 3: mouse spleen tissue lysate](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-JJ0930-Western-Blot-NBP2-67909-img0004.jpg)
![Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of paraffin-embedded human lung cancer tissue using anti-DUSP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-JJ0930-Immunohistochemistry-Paraffin-NBP2-67909-img0002.jpg)
![Western Blot: MKP-1/DUSP1 Antibody [NB100-834] - Staining of HeLa cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-Western-Blot-NB100-834-img0004.jpg "Western Blot: MKP-1/DUSP1 Antibody [NB100-834] - Staining of HeLa cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody [NB100-834] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-Immunohistochemistry-Paraffin-NB100-834-img0006.jpg)
![Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody [NB100-834] - Staining of paraffin embedded Human Prostate. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-Immunohistochemistry-Paraffin-NB100-834-img0005.jpg)
![ELISA: Human MKP-1/DUSP1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31428] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31428_human-mkp-1-dusp1-ready-to-use-elisa-kit-colorimetric-20620241527375.png "ELISA: Human MKP-1/DUSP1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31428] - Standard Curve Reference")
![Immunocytochemistry/Immunofluorescence: MKP-1/DUSP1 Antibody (3A9) [H00001843-M05] - Analysis of monoclonal antibody to DUSP1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-3A9-Immunocytochemistry-Immunofluorescence-H00001843-M05-img0002.jpg "Immunocytochemistry/Immunofluorescence: MKP-1/DUSP1 Antibody (3A9) [H00001843-M05] - Analysis of monoclonal antibody to DUSP1 on HeLa cell. Antibody concentration 10 ug/ml")
![Sandwich ELISA: MKP-1/DUSP1 Antibody (3A9) [H00001843-M05] - Detection limit for recombinant GST tagged DUSP1 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-3A9-Sandwich-ELISA-H00001843-M05-img0003.jpg)
![Proximity Ligation Assay: MKP-1/DUSP1 Antibody (4H7) [H00001843-M02] - Analysis of protein-protein interactions between MAPK3 and DUSP1. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-DUSP1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-4H7-Proximity-Ligation-Assay-H00001843-M02-img0001.jpg "Proximity Ligation Assay: MKP-1/DUSP1 Antibody (4H7) [H00001843-M02] - Analysis of protein-protein interactions between MAPK3 and DUSP1. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-DUSP1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![Sandwich ELISA: MKP-1/DUSP1 Antibody (4H7) [H00001843-M02] - Detection limit for recombinant GST tagged DUSP1 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-4H7-Sandwich-ELISA-H00001843-M02-img0002.jpg)
![Western Blot: MKP-1/DUSP1 Antibody [NBP2-57354] - Analysis in human cell line U-2 OS.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-Western-Blot-NBP2-57354-img0003.jpg "Western Blot: MKP-1/DUSP1 Antibody [NBP2-57354] - Analysis in human cell line U-2 OS.")
![Immunocytochemistry/Immunofluorescence: MKP-1/DUSP1 Antibody [NBP2-57354] - Staining of human cell line SiHa shows localization to nucleoli & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57354-img0001.jpg)
![SDS-Page: MKP-1/DUSP1 Recombinant Protein [H00001843-P01]](http://images.novusbio.com/fullsize/MKP-1-DUSP1-Recombinant-Protein-SDS-Page-H00001843-P01-img0001.jpg "SDS-Page: MKP-1/DUSP1 Recombinant Protein [H00001843-P01]")
![ELISA: Human MKP-1/DUSP1 ELISA Kit (Colorimetric) [NBP3-31426] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31426_human-mkp-1-dusp1-elisa-kit-colorimetric-206202415294097.png "ELISA: Human MKP-1/DUSP1 ELISA Kit (Colorimetric) [NBP3-31426] - Standard Curve Reference")
