MKK7/MEK7 Antibody (SD20-87)

Western Blot: MKK7/MEK7 Antibody (SD20-87) [NBP2-67764] - Analysis of MEK7 on different lysates using anti-MEK7 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: A431

Immunocytochemistry/Immunofluorescence: MKK7/MEK7 Antibody (SD20-87) [NBP2-67764] - Staining MEK7 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MKK7/MEK7 Antibody (SD20-87) [NBP2-67764] - Analysis of paraffin-embedded rat brain tissue using anti-MEK7 antibody. Counter stained with hematoxylin.

Flow Cytometry: MKK7/MEK7 Antibody (SD20-87) [NBP2-67764] - Analysis of Hela cells with MEK7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: MKK7/MEK7 Antibody (SD20-87) [NBP2-67764] - Staining MEK7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: MKK7/MEK7 Antibody (SD20-87) [NBP2-67764] - Staining MEK7 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MKK7/MEK7 Antibody (SD20-87) [NBP2-67764] - Analysis of paraffin-embedded mouse bladder tissue using anti-MEK7 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MKK7/MEK7 Antibody (SD20-87) [NBP2-67764] - Analysis of paraffin-embedded rat bladder tissue using anti-MEK7 antibody. Counter stained with hematoxylin.

Product Discontinued

MKK7/MEK7 Antibody (SD20-87) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human MKK7/MEK7 aa 370-419 / 419. (SwissProt: O14733 Human; SwissProt: Q8CE90 Mousen; SwissProt: Q4KSH7 Rat)
• Localization: Nucleus, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAP2K7
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MKK7/MEK7 Antibody (SD20-87)

• c-Jun N-terminal kinase kinase 2
• dual specificity mitogen-activated protein kinase kinase 7
• EC 2.7.12.2
• JNK kinase 2
• JNK-activating kinase 2
• JNKK 2
• JNKK2
• MAP kinase kinase 7
• MAP2K7
• MAPK/ERK kinase 7
• MAPKK7
• MEK 7
• MEK7
• mitogen-activated protein kinase kinase 7
• MKE7
• MKK7
• MKK7MAPKK 7
• PRKMK7

Background

c-Jun NH2-terminal protein kinase (JNK), a distant member of the mitogen-activated protein (MAP) kinase family, regulates gene expression in response to various extracellular stimuli. MKK7, a novel member of the MAP kinase kinase family, was phosphorylated and activated by MEKK1, a MAP kinase kinase kinase in the JNK signaling cascade. MKK7 activity was also stimulated by constitutively active forms of Rac and Cdc42Hs, members of the Rho small GTP-binding protein family (1). endogenous MKK7 was activated by treatment with the growth factor interleukin-3 (but not interleukin-4), or by ligation of CD40, the B-cell antigen receptor, or the receptor for the Fc fragment of immunoglobulin. MKK7 was also activated when cells were exposed to heat, UV irradiation, anisomycin, hyperosmolarity or the pro-inflammatory cytokine tumor necrosis factor-alpha, suggesting that MKK7 is involved in many physiological pathways (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for MKK7/MEK7 Antibody (NBP2-67764). (Showing 1 - 1 of 1 FAQ).

1. What area of Human MEK7 was used in generating MKK7/MEK7 Antibody (SD20-87) NBP2-67764?
   • The exact immunogen for the monoclonal antibody NBP2-67764 is the C terminal Human MEK7 (aa 401-419, TESPRTSGVLSQPHLPFFR).

Bioinformatics

• Gene Symbol: MAP2K7