MKK6/MEK6 Antibody [CoraFluor™ 1]

• Reactivity: Hu, Mu, Rt
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: CoraFluor 1

MKK6/MEK6 Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant human MKK6
  Ser2-Asp334
  Accession # P52564
• Specificity: Detects human, mouse, and rat MKK6.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MAP2K6
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for MKK6/MEK6 Antibody [CoraFluor™ 1]

• MAP kinase kinase 6
• MAP2K6
• MAPK/ERK kinase 6
• MAPKK6dual specificity mitogen-activated protein kinase kinase 6
• MEK6
• MEK6MAPKK 6
• mitogen-activated protein kinase kinase 6
• MKK6
• MKK6MEK 6
• PRKMK6
• PRKMK6protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6)
• SAPKK3
• SAPKK3EC 2.7.12.2

Background

Mitogen-acitvated protein kinase kinase 6 (MKK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1-2). MKK6, closely related to MKK3, efficiently phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines and environmental stress but not JNK or ERK (3). MTK1 phosphorylates MKK6 on serine 207 and threonine 211 (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP2K6