MKK6/MEK6 Antibody [Alexa Fluor® 594]

• Reactivity: Hu, Mu, Rt
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 594

MKK6/MEK6 Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human MKK6
  Ser2-Asp334
  Accession # P52564
• Specificity: Detects human, mouse, and rat MKK6.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MKK6/MEK6 Antibody [Alexa Fluor® 594]

• MAP kinase kinase 6
• MAP2K6
• MAPK/ERK kinase 6
• MAPKK6dual specificity mitogen-activated protein kinase kinase 6
• MEK6
• MEK6MAPKK 6
• mitogen-activated protein kinase kinase 6
• MKK6
• MKK6MEK 6
• PRKMK6
• PRKMK6protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6)
• SAPKK3
• SAPKK3EC 2.7.12.2

Background

Mitogen-activated protein kinase kinase 6 (MKK6), also known as MEK6, and the closely related MKK3 are activated by pro-inflammatory cytokines and environmental stresses (1, 2). Both MKK3 and MKK6 are dual specificity protein kinases, phosphorylating and activating the p38 MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr (3). Activation of MKK6 occurs through phosphorylation at Ser207 and Thr211 (4) by several upstream MAPK kinase kinases, including ASK1 and TAK1 (5, 6). Purification of E. coli-expressed wild-type active MKK6 capable of p38 alpha phosphorylation has been previously noted (7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.