Mitofilin Antibody (H6/C12)

Product Discontinued

Mitofilin Antibody (H6/C12) Summary

• Immunogen: Human mitochondrial membrane preparation.
• Marker: Mitochondria Marker
• Specificity: Recognizes an antigen expressed on the inner membrane of mitochondria which is partially sensitive to proteinase digestion.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: IMMT
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA 1:50
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tissue culture supernatant
• Preservative: 0.09% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for Mitofilin Antibody (H6/C12)

• DKFZp779P1653
• inner membrane protein, mitochondrial
• MGC111146
• Mic 60
• Mic60
• MICOS Complex Subunit MIC60
• mitochondrial (mitofilin)
• mitofilin
• motor protein
• p87/89
• proliferation-inducing gene 4

Background

The inner mitochondrial membrane forms internal compartments known as cristae and holds the essential proteins (NADH dehydrogenase, cytochrome c reductase, cytochrome c oxidase) involved in the respiratory chain, facilitating the production of ATP from NADH.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Mitofilin Antibody (NB100-64695) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer WB Rat 12/10/2016

Summary

• Application: Western Blot
• Sample Tested: Brain
• Species: Rat
• Lot: 010507

Comments

• Comments: Per the Novus Innovator's Reward, we decided to test whether this Mitofilin Supernatant product would work well for Western Blot, seeing that it worked well for other applications in our target species Rat.
  The gel was loaded with 15 ug per lane of brain lysate. PVDF membranes were blocked with 5% milk for 1 h, and incubated with Mitofilin H6/C12 overnight at 4C. Anti mouse-HRP secondary was applied for 2h at room temp, with blots developed using high-sensitivity Supersignal West Femto ECL reagent. We attempted using this antibody at 1:200 and 1:40 (shown here), with no signal detected. The same blot was successfully tested with known good antibody, which worked, pointing to successful transfer and detection.

Bioinformatics

• Gene Symbol: IMMT