Recombinant Human MIS12 His Protein

SDS-Page: MIS12 Protein [NBP2-23237]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human MIS12 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-205 of Human MIS12
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSVDPMT YEAQFFGFTP QTCMLRIYIA FQDYLFEVMQ AVEQVILKKL DGIPDCDISP VQIRKCTEKF LCFMKGHFDN LFSKMEQLFL QLILRIPSNI LLPEDKCKET PYSEEDFQHL QKEIEQLQEK YKTELCTKQA LLAELEEQKI VQAKLKQTLT FFDELHNVGR DHGTSDFRES LVSLVQNSRK LQNIRDNVEK ESKRLKIS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MIS12
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 26.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 2M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human MIS12 His Protein

• 2510025F08Rik
• hMis12
• homolog of yeast Mis12
• KNTC2AP
• MGC2488
• MIS12 homolog
• MIS12, MIND kinetochore complex component, homolog (S. pombe)
• MIS12, MIND kinetochore complex component, homolog (yeast)
• MTW1
• protein MIS12 homolog

Background

Protein MIS12 homolog, also known as MIS12, is a component of the MIS12 complex, which is required for kinetochore formation during mitosis and normal chromosome alignment and segregation. The MIS12 complex consists of MIS12, DSN1, NSL1 and PMF-1. MIS12 is part of a network of complexes that provide microtubule attachment and generates pulling forces from depolymerization. Recombinant human MIS12 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MIS12