Mind Bomb 2/MIB2 Antibody (739505) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB
• Clone: 739505
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

Mind Bomb 2/MIB2 Antibody (739505) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human Mind Bomb 2/MIB2
  Ala418-Lys520
  Accession # Q96AX9
• Specificity: Detects human Mind Bomb 2/MIB2 in direct ELISAs and Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mind Bomb 2/MIB2 Antibody (739505) [Alexa Fluor® 532]

• E3 ubiquitin-protein ligase MIB2
• EC 6.3.2
• EC 6.3.2.-
• EC 6.3.2.13
• FLJ20648
• FLJ41406
• MIB2
• Mind Bomb 2
• Mind bomb homolog 2
• Mind Bomb-2
• mindbomb homolog 2 (Drosophila)
• Novel zinc finger protein
• Novelzin
• Putative NF-kappa-B-activating protein 002N
• SKD
• Skeletrophin
• Zinc finger ZZ type with ankyrin repeat domain protein 1
• ZZ type with ankyrin repeat domain 1
• ZZANK1
• ZZZ5

Background

MIB-2 (Mind Bomb-2), also called skeletrophin, is a widely expressed, 1013 amino acid (aa) cytoplasmic phosphoprotein that is an E3 ubiquitin ligase for Notch ligands such as Jagged-2. It contains two MIB/HERC2 domains surrounding a ZZ-type zinc finger (aa 59-285), an ankyrin repeat domain (aa 522-856), and two RING-type zinc fingers that have E3 ubiquitin ligase activity (aa 890-1002). If short gaps in the protein sequence are ignored, human MIB-2 (aa 418-520) shares 95% and 92% aa identity with mouse and rat MIB-2, respectively. Eight alternately spliced isoforms of 672-1009 aa lack various sequences, but all contain aa 418-520. MIB-2 has been detected as a 70 kDa form in skeletal muscle, binds actin, and is downregulated in malignant melanoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.