Mind Bomb 2/MIB2 Antibody (739505) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB
• Clone: 739505
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

Mind Bomb 2/MIB2 Antibody (739505) [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human Mind Bomb 2/MIB2
  Ala418-Lys520
  Accession # Q96AX9
• Specificity: Detects human Mind Bomb 2/MIB2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mind Bomb 2/MIB2 Antibody (739505) [Alexa Fluor® 405]

• E3 ubiquitin-protein ligase MIB2
• EC 6.3.2
• EC 6.3.2.-
• EC 6.3.2.13
• FLJ20648
• FLJ41406
• MIB2
• Mind Bomb 2
• Mind bomb homolog 2
• Mind Bomb-2
• mindbomb homolog 2 (Drosophila)
• Novel zinc finger protein
• Novelzin
• Putative NF-kappa-B-activating protein 002N
• SKD
• Skeletrophin
• Zinc finger ZZ type with ankyrin repeat domain protein 1
• ZZ type with ankyrin repeat domain 1
• ZZANK1
• ZZZ5

Background

MIB-2 (Mind Bomb-2), also called skeletrophin, is a widely expressed, 1013 amino acid (aa) cytoplasmic phosphoprotein that is an E3 ubiquitin ligase for Notch ligands such as Jagged-2. It contains two MIB/HERC2 domains surrounding a ZZ-type zinc finger (aa 59-285), an ankyrin repeat domain (aa 522-856), and two RING-type zinc fingers that have E3 ubiquitin ligase activity (aa 890-1002). If short gaps in the protein sequence are ignored, human MIB-2 (aa 418-520) shares 95% and 92% aa identity with mouse and rat MIB-2, respectively. Eight alternately spliced isoforms of 672-1009 aa lack various sequences, but all contain aa 418-520. MIB-2 has been detected as a 70 kDa form in skeletal muscle, binds actin, and is downregulated in malignant melanoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q96AX9(Human)