Migfilin Antibody (OTI3G1) - Azide and BSA Free

Western Blot: Migfilin Antibody (OTI3G1) - Azide and BSA Free [NBP2-72691] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Migfilin.

Western Blot: Migfilin Antibody (OTI3G1) - Azide and BSA Free [NBP2-72691] - Analysis of extracts (10ug) from 2 different cell lines.

• Reactivity: Hu
• Applications: WB
• Clone: OTI3G1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Migfilin Antibody (OTI3G1) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Migfilin Antibody (OTI3G1) - Azide and BSA Free (NBP2-45595) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-242 of human FBLIM1(NP_060026) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FBLIM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
• Theoretical MW: 40.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Migfilin Antibody (OTI3G1) - Azide and BSA Free

• CAL
• DKFZp434G171
• FBLP-1FBLP1CSX-associated LIM
• filamin binding LIM protein 1
• filamin-binding LIM protein 1
• filamin-binding LIM protein-1
• MIG2-interacting protein
• migfilin
• mitogen-inducible 2 interacting protein
• Mitogen-inducible 2-interacting protein

Background

FBLIM1 encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FBLIM1