MICL/CLEC12A Antibody (687317) [Unconjugated]

Human peripheral blood granulocytes were stained with Mouse Anti-Human MICL/CLEC12A Monoclonal Antibody (Catalog # MAB2946, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 687317
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MICL/CLEC12A Antibody (687317) [Unconjugated] Summary

• Additional Information: Clone 687317 was used by HLDA to establish CD designation
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MICL/CLEC12A
  Thr67-Ala265
  Accession # Q5QGZ9
• Specificity: Detects human MICL/CLEC12A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CLEC1, 2, 2A, 3B, 9A, 10A, 12B, 14A, rhCD302/CLEC13A recombinant mouse (rm) CLEC4B2, or rmMICL is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC12A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MICL/CLEC12A Antibody (687317) [Unconjugated]

• CD371
• CLEC12A
• CLL1
• CLL-1
• C-type lectin domain family 12, member A
• DCAL-2
• MICL

Background

Human MICL (myeloid inhibitory C-type lectin-like receptor; also CLL-1 and KLRL-1) is a 75 kDa member of the group V C-type lectin-like receptor family of molecules. Group V molecules have a lectin-type domain that binds non-sugar ligands. MICL is a 265 amino acid (aa) type II transmembrane (TM) glycoprotein that contains a 200 aa extracellular domain (ECD). Multiple isoforms of MICL are reported. One potentially utilizes an alternate start site that adds 10 aa to the cytoplasmic domain. There is one potential soluble form that lacks the TM segment, and two truncated ECD isoforms. Human MICL ECD is 53%, 65%, and 55% aa identical to the ECD in mouse, dog and cow, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MICL/CLEC12A Antibody (MAB2946)(1)

Publication using MAB2946

• CLEC12A-Mediated Antigen Uptake and Cross-Presentation by Human Dendritic Cell Subsets Efficiently Boost Tumor-Reactive T Cell Responses J Immunol, 2016-08-26;0(0):. 2016-08-26 [PMID: 27566820] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CLEC12A
• Uniprot:
  • Q5QGZ9()