MICALL1 Antibody - Azide and BSA Free

Western Blot: MICALL1 Antibody [H00085377-B01P] - Analysis of MICALL1 expression in transfected 293T cell line by MICALL1 polyclonal antibody. Lane 1: MICAL-L1 transfected lysate(94.93 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: MICALL1 Antibody [H00085377-B01P] - GRAF/WDR44 label a subset of tubular endosomes. Insets show magnifications of the boxed areas. Scale bars: 10 um; scale bars of insets: 2 um. Image collected and cropped by CiteAb from the following publication (//rupress.org/jcb/article/doi/10.1083/jcb.201811014/151714/GRAF2-WDR44-and-MICAL1-mediate-Rab81011dependent) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: MICALL1 Antibody [H00085377-B01P] - IHC staining of MICALL1 in human prostate cancer using DAB with hematoxylin counterstain.

Western Blot: MICALL1 Antibody [H00085377-B01P] - Analysis of MICALL1 expression in HepG2.

Immunocytochemistry/ Immunofluorescence: MICALL1 Antibody [H00085377-B01P] - GRAF/WDR44 label a subset of tubular endosomes. (A–C) HeLa cells were incubated with methanol (vehicle) or BFA (5 µg/ml) for 5 or 15 min. (A) Percentage of cells with endogenous (endo.) WDR44 tubules. n = 7–25. (B) Confocal images of cells stained with alpha -WDR44 & alpha -TGN46 or preincubated with Alexa Fluor 546–Transferrin (10 µg/ml, 1 h). (C) Manders colocalization coefficients for the indicated proteins with endogenous WDR44 structures. n = 10–50 cells. (D) Confocal images of transfected HeLa cells showing colocalization of RFP-WDR44 with GFP-STX16 & GFP-VAMP3. (E) Immunoprecipitation (IP) of transfected 293T cells with alpha -GFP. uGRAF2 was coimmunoprecipitated by GFP-MICAL1, but not by any other member of the MICAL family. (F) Confocal images of transfected HeLa cells stained with alpha -WDR44. In the case of MICAL3pF1KA0819 & MICAL-L2, boxed areas show tubules positive only for WDR44 (red), only for MICAL3pF1KA0819/MICAL-L2 (cyan), or shared by the two proteins (white). (G) Percentage of transfected HeLa cells with endogenous (endo.) WDR44 tubules. n = 4. (H) Confocal images of untransfected HeLa cells stained with alpha -WDR44 & alpha -MICAL-L1. Boxed areas show tubules positive only for WDR44 (red), only for MICAL-L1 (cyan), or shared by the two proteins (white). (I) Internalized Integrin-beta 1 in shRNA-expressing HeLa cells after uptake of alpha -Integrin-beta 1 & following a 4-h chase. n = 4. (A, C, G, & I) Data are means ± SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001; & ****, P < 0.0001. (B, D, F, & H) Insets show magnifications of the boxed areas. Scale bars: 10 µm; scale bars of insets: 2 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32344433), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MICALL1 Antibody - Azide and BSA Free Summary

• Immunogen: MICAL-L1 (AAI48805.1, 1 a.a. - 863 a.a.) full-length human protein. MAGPRGALLAWCRRQCEGYRGVEIRDLSSSFRDGLAFCAILHRHRPDLLDFDSLSKDNVFENNRLAFEVAEKELGIPALLDPNDMVSMSVPDCLSIMTYVSQYYNHFCSPGQAGVSPPRKGLAPCSPPSVAPTPVEPEDVAQGEELSSGSLSEQGTGQTPSSTCAACQQHVHLVQRYLADGRLYHRHCFRCRRCSSTLLPGAYENGPEEGTFVCAEHCARLGPGTRSGTRPGPFSQPKQQHQQQLAEDAKDVPGGGPSSSAPAGAEADGPKASPEARPQIPTKPRVPGKLQELASPPAGRPTPAPRKASESTTPAPPTPRPRSSLQQENLVEQAGSSSLVNGRLHELPVPKPRGTPKPSEGTPAPRKDPPWITLVQAEPKKKPAPLPPSSSPGPPSQDSRQVENGGTEEVAQPSPTASLESKPYNPFEEEEEDKEEEAPAAPSLATSPALGHPESTPKSLHPWYGITPTSSPKTKKRPAPRAPSASPLALHASRLSHSEPPSATPSPALSVESLSSESASQTAGAELLEPPAVPKSSSEPAVHAPGTPGNPVSLSTNSSLASSGELVEPRVEQMPQASPGLAPRTRGSSGPQPAKPCSGATPTPLLLVGDRSPVPSPGSSSPQLQVKSSCKENPFNRKPSPAASPATKKATKGSKPVRPPAPGHGFPLIKRKVQADQYIPEEDIHGEMDTIERRLDALEHRGVLLEEKLRGGLNEGREDDMLVDWFKLIHEKHLLVRRESELIYVFKQQNLEQRQADVEYELRCLLNKPEKDWTEEDRAREKVLMQELVTLIEQRNAIINCLDEDRQREEEEDKMLEAMIKKKEFQREAEPEGKKKGKFKTMKMLKLLGNKRDAKSKSPRDKS
• Specificity: MICAL-L1 - MICAL-like 1,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: MICALL1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:400
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. Antibody is also useful for Immunohistochemistry on paraffin-embedded sections. Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Immunoprecipitation were reported in scientific literature, but Immunocytochemistry/Immunofluorescence has been experiencing lot-to-lot variations. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 24019528).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MICALL1 Antibody - Azide and BSA Free

• DKFZp686M2226
• KIAA1668
• MICAL-L1
• MICAL-like 1
• MICAL-like protein 1

Background

MICALL1 may be a cytoskeletal regulator

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MICALL1 Antibody (H00085377-B01P)(25)

Publications using H00085377-B01P

• Rangaraj N, Vaibhava V, Sudhakar C et al. Transferrin-induced signaling through transferrin receptor and AKT kinase mediates formation of Rab8- and MICAL-L1-positive tubules involved in receptor recycling bioRxiv 2023-02-07 (ICC/IF, Human)
• Bali L, Christopher H, Rose W et al. Disruptions in endocytic traffic contribute to the activation of the NLRP3 inflammasome. Sci Signal. 2023-02-21 [PMID: 36809026]
• Finicle BT Targeting endolysosomal trafficking with synthetic sphingolipid analogs to improve the delivery of oligonucleotide therapeutics Thesis 2023-01-01 (ICC/IF)
• Dhawan K, Naslavsky N, Caplan S Coronin2A links actin-based endosomal processes to the EHD1 fission machinery Molecular biology of the cell 2022-08-03 [PMID: 35921168]
• Mamais A, Kluss JH, Bonet-Ponce L et al. Mutations in LRRK2 linked to Parkinson disease sequester Rab8a to damaged lysosomes and regulate transferrin-mediated iron uptake in microglia PLoS biology 2021-12-01 [PMID: 34914695] (ICC/IF, Human)
• Lucken-Ardjomande HAsler S, Vallis Y, Pasche M, McMahon HT GRAF2, WDR44, and MICAL1 mediate Rab8/10/11-dependent export of E-cadherin, MMP14, and CFTR delta, F508 J. Cell Biol. 2020-05-04 [PMID: 32344433] (WB, Human)
• Siddiqa A, Massimi P, Pim D, Banks L Diverse Papillomavirus Types Induce Endosomal Tubulation Front Cell Infect Microbiol 2019-05-28 [PMID: 31192164] (ICC/IF, Human)
• Fukuda M Rab10 regulates tubular endosome formation through KIF13A/B motors. J Cell Sci. 2019-02-19 [PMID: 30700496]
• R Sakai, R Fukuda, S Unida, M Aki, Y Ono, A Endo, S Kusumi, D Koga, T Fukushima, M Komada, T Okiyoneda The integral function of endocytic recycling compartment is regulated by RFFL-mediated ubiquitination of Rab11 effectors J. Cell. Sci., 2019-02-07;0(0):. 2019-02-07 [PMID: 30659120]
• Xie S, Reinecke JB, Farmer T et al. Vesicular trafficking plays a role in centriole disengagement and duplication Mol. Biol. Cell 2018-11-01 [PMID: 30188792] (WB, Human)
• Finicle BT, Ramirez MU, Liu G et al. Sphingolipids inhibit endosomal recycling of nutrient transporters by inactivating ARF6. J Cell Sci 2018-06-25 [PMID: 29848659] (Human)
• Mukadam AS, Breusegem SY, Seaman MNJ. Analysis of novel endosome-to-Golgi retrieval genes reveals a role for PLD3 in regulating endosomal protein sorting and amyloid precursor protein processing Cell. Mol. Life Sci. 2018-01-24 [PMID: 29368044] (WB, Human)
• Siddiqa A, Massimi P, Pim D et al. Human Papillomavirus 16 infection induces VAP-dependent endosomal tubulation J. Virol. 2018-01-10 [PMID: 29321327] (Human)
• Vidal-Quadras M, Holst MR, Francis MK et al. Endocytic turnover of Rab8 controls cell polarization. J. Cell. Sci. 2017-03-15 [PMID: 28137756] (WB, Human)
• Bahl K, Xie S, Spagnol G et al. EHD3 is Required for Tubular Recycling Endosome Stabilization and an Asparagine-Glutamic Acid Residue Pair within its EH Domain Dictates its Selective Binding to NPF Peptides. J. Biol. Chem. 2016-05-04 [PMID: 27189942] (Human)
• Reinecke Jb, Katafiasz D, Naslavsky N, Caplan S. Novel Functions for the Endocytic Regulatory Proteins MICAL-L1 AnD EHD1 in Mitosis. Traffic. 2014-10-07 [PMID: 25287187] (ICC/IF, WB, Human)
• Xie S, Naslavsky N, Caplan S. Diacylglycerol kinase alpha regulates tubular recycling endosome biogenesis and major histocompatibility complex class I recycling. J Biol Chem. 2014-09-23 [PMID: 25248744]
• Kobayashi H, Etoh K, Ohbayashi N, Fukuda M. Rab35 promotes the recruitment of Rab8, Rab13 and Rab36 to recycling endosomes through MICAL-L1 during neurite outgrowth. Biol Open. 2014-08-01 [PMID: 25086062]
• Cai B, Giridharan SS, Zhang J et al. Differential Roles of C-terminal Eps15 Homology Domain Proteins as Vesiculators and Tubulators of Recycling Endosomes. J Biol Chem. 2013-10-18 [PMID: 24019528] (ICC/IF, Human)
• Giridharan SS, Cai B, Vitale N et al. Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis. Mol Biol Cell. 2013-04-17 [PMID: 23596323] (WB, ICC/IF, IP, Human)
• Kobayashi H, Fukuda M. Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth. J Cell Sci. 2013-04-09 [PMID: 23572513]
• Cai B, Caplan S, Naslavsky N. cPLA2? and EHD1 interact and regulate the vesiculation of cholesterol-rich, GPI-anchored, protein-containing endosomes. Mol Biol Cell;23(10):1874-88. 2012-05-01 [PMID: 22456504] (ICC/IF, Human)
• Rahajeng J, Panapakkam Giridharan SS, Cai B, Naslavsky N, Caplan S. MICAL-L1 is a tubular endosomal membrane hub that connects Rab35 and Arf6 with Rab8a. Traffic;13(1):82-93. 2012-01-01 [PMID: 21951725] (ICC/IF, Human)
• Rahajeng J, Giridharan SS, Naslavsky N, Caplan S. Collapsin response mediator protein-2 (Crmp2) regulates trafficking by linking endocytic regulatory proteins to dynein motors. J Biol Chem 2010-10-15 [PMID: 20801876] (ICC/IF, Human)
• Sharma M, Giridharan SS, Rahajeng J et al. MICAL-L1 links EHD1 to tubular recycling endosomes and regulates receptor recycling. Mol Biol Cell 2009-12-01 [PMID: 19864458] (IF/IHC)

Bioinformatics

• Gene Symbol: MICALL1
• Entrez:
  • 85377(Human)
• Uniprot:
  • Q8N3F8(Human)