| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65541SS | |||
| Azide and BSA Free | NBP2-80849 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-6405SS | |||
| Azide and BSA Free | NBP2-80848 | |||
VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing central nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="IL-1 RI was detected in perfusion fixed frozen sections of mouse skin (tail) using Goat Anti-Mouse IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF771) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing central nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF771-SP | |||
263-2R) inhibits Recombinant Human IL-1 beta / IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 RII Monoclonal Antibody (Catalog # MAB263). The ND50 is typically 2-6 µg/mL." alt="Recombinant Human IL-1 RII (263-2R) inhibits Recombinant Human IL-1 beta / IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 RII Monoclonal Antibody (Catalog # MAB263). The ND50 is typically 2-6 µg/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB263-SP | |||
AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." alt="HUVEC human umbilical vein endothelial cells were labeled with Human IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF269, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB269F-100 | |||
| Carrier Free | FAB269P-100 | |||
| Carrier Free | FAB269A-100 | |||
| Carrier Free | FAB269N-100 | |||
| Carrier Free | FAB269N-025 | |||
| Carrier Free | FAB269A-025 | |||
| Carrier Free | FAB269P-025 | |||
| Carrier Free | AF269-SP | |||
| Carrier Free | FAB269F-025 | |||
263-2R) inhibits Recombinant Human IL-1 beta /IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-263-NA). The ND50 is typically 15‑30 µg/mL." alt="Recombinant Human IL-1 RII (263-2R) inhibits Recombinant Human IL-1 beta /IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-263-NA). The ND50 is typically 15‑30 µg/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF-263-SP | |||
AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." alt="EL-4 mouse lymphoblast cell line was stained with Goat Anti-Mouse IL-1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF563, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF563-SP | |||
263-2R) inhibits Recombinant Human IL-1 beta /IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Human IL-1 RII Monoclonal Antibody (Catalog # MAB663). The ND50 is typically 5-20 µg/mL." alt="Recombinant Human IL-1 RII (263-2R) inhibits Recombinant Human IL-1 beta /IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Human IL-1 RII Monoclonal Antibody (Catalog # MAB663). The ND50 is typically 5-20 µg/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB663F | |||
| Carrier Free | FAB663P | |||
| Carrier Free | FAB663A | |||
| Carrier Free | FAB663N | |||
| Carrier Free | MAB663-SP | |||
| Carrier Free | MAB663-500 | |||
![Western Blot: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Total protein from mouse placenta and spleen was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RT1b in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Western-Blot-NB100-65541-img0011.jpg)
![Immunohistochemistry-Paraffin: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using MHC Class II RT1B antibody (clone OX-6) at 1:100 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the nuclei were counterstained with hematoxylin. The antibody generated very specific signal in a subset of spleenocytes.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Immunohistochemistry-Paraffin-NB100-65541-img0014.jpg)
![Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Staining of rat spleen cells.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Flow-Cytometry-NB100-65541-img0005.jpg)
![Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Analysis using Alexa Fluor (R) 488 conjugate of NB100-65541. Rat Splenocytes were stained with MHC Class II RT1B (OX-6) antibody NB100-65541 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Flow-Cytometry-NB100-65541-img0012.jpg)
![Western Blot: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Rat Spleen Tissue lysate probed with MHC Class II antibody.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Western-Blot-NB100-65541-img0002.jpg)
![Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] - Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B](http://images.novusbio.com/fullsize/nb100-65541_mouse-monoclonal-mhc-class-ii-rt1b-antibody-ox-6-17720241044625.jpg)
![Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] - Staining of rat spleen cells.](http://images.novusbio.com/fullsize/nb100-65541_mouse-monoclonal-mhc-class-ii-rt1b-antibody-ox-6-17720241044643.jpg)
![Immunocytochemistry/ Immunofluorescence: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] - Administration of cord blood cells with plasma in the 6-OHDA PD rat model: Assessment of immune cell activation, & pro-inflammatory cytokine production in the intestine. Confocal imaging shows a significant reduction in OX-6 (MHC II) expression in the intestinal mucosa of 6-OHDA animals that were given a combination of cord blood cells & plasma. This therapy also resulted in a significant decrease in TNF expression in this animal model. Quantitative analyses of the estimated OX-6 expression & TNF are displayed in the graphs above. Representative merged images above show co-localization of OX-6 (Green) or TNF (Red) with DAPI+ (Blue) expression from cells in the small intestine of 6-OHDA PD animals. Arrow heads indicate positive staining of OX-6 & TNF expression in intestinal villi. Scale bar = 100 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31317505), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-65541_mouse-monoclonal-mhc-class-ii-rt1b-antibody-ox-6-310202415532025.jpg)
![Immunocytochemistry/ Immunofluorescence: MHC Class II RT1B Antibody (OX-6) - BSA Free [NB100-65541] - Administration of cord blood cells with plasma in the 6-OHDA PD rat model: Assessment of TH-positive dopaminergic neurons, immune cell activation, & pro-inflammatory cytokine production. Tyrosine hydroxylase (TH) staining (top row) was used to investigate the potential effect of combined cord blood cell with plasma injection on dopaminergic neuron populations in the substantia nigra pars compacta (SNpc). Analysis of fluorescent intensity revealed significant protection & retention of dopaminergic cells in the SNpc in 6-OHDA animals treated with cord blood cells & plasma, compared to vehicle controls. Cord blood treated groups also demonstrated significant reductions immune cell activation (OX-6) & pro-inflammatory cytokine production (TNF). Overall, the combination of cord blood cells & plasma can significantly modulate the exacerbated immune response through downregulation of pro-inflammatory cytokine production & immune cell activation in the 6-OHDA rat model at all time points tested. Photomicrographs correspond to representative SN in coronal sections immune-labeled with TH, OX-6 or TNF antibody. Scale bar = 50 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31317505), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-65541_mouse-monoclonal-mhc-class-ii-rt1b-antibody-ox-6-310202415523917.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MHC Class I Antibody (OX18) [NB120-6405] - Analysis of FFPE rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Endothelial staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-OX18-Immunohistochemistry-Paraffin-NB120-6405-img0008.jpg)
![Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-OX18-Flow-Cytometry-NB120-6405-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-MHC Class I (OX18) NB120-6405 at a 1:100 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-OX18-Immunocytochemistry-Immunofluorescence-NB120-6405-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC12 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MHC Class I Antibody (OX18) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-OX18-Immunocytochemistry-Immunofluorescence-NB120-6405-img0010.jpg)
![Flow (Intracellular): MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were stained with MHC Class I NB120-6405 (Blue) and a matched isotype control NBP2-27287 (Orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by DyLight488-conjugated anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-OX18-Flow-Intracellular-NB120-6405-img0004.jpg)
![Immunohistochemistry: MHC Class I Antibody (OX18) - BSA Free [NB120-6405] - Effects of anodal tDCS on protein expression. Representative immunohistochemical images of Ox18 + cells in the SVZ ipsilateral to anodal tDCS or sham stimulation. Staining for Ox18 (MHC I) in the ipsilateral SVZ revealed more Ox18 + cells in animals treated by anodal tDCS (left panel) compared to sham stimulation (right panel) by trend (scale bars = 100 μm). Results are displayed as mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31708742), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-6405_mouse-monoclonal-mhc-class-i-antibody-ox18-31020241534348.jpg)
400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1a/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF771). The ND50 is typically 0.2-1.0 µg/mL." class="big_thumb" />
![N/A IL-1 RII [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_IL1_RII_DR1B00_ELISA_799.jpg "N/A IL-1 RII [HRP]")
![N/A IL-1 RII [HRP]](http://images.novusbio.com/fullsize/elisa/IL-1_RII_DR1B00_ELISA_166.jpg)
NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for 
MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # 
201-LB) induces CXCL1/GROa secretion in the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line),as measured by the Human CXCL1/GROa DuoSet (Catalog # 
269-1R) was serially diluted and captured by Mouse Anti-Human IL‑1 RI Monoclonal Antibody (Catalog # 
263-2R) was serially diluted and captured by Mouse Anti-Human IL‑1 RII Monoclonal Antibody (Catalog # 
CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for ![Western Blot: IL-1 RII Antibody [NBP1-32681] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IL1 Receptor 2 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-1-RII-Antibody-Western-Blot-NBP1-32681-img0012.jpg "Western Blot: IL-1 RII Antibody [NBP1-32681] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IL1 Receptor 2 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: IL-1 RII Antibody [NBP1-32681] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/IL-1-RII-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32681-img0006.jpg)
![Immunohistochemistry-Paraffin: IL-1 RII Antibody [NBP1-32681] - Human lung cancer. IL1 Receptor 2 antibody [N3C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/IL-1-RII-Antibody-Immunohistochemistry-Paraffin-NBP1-32681-img0009.jpg)
![Immunohistochemistry-Paraffin: IL-1 RII Antibody [NBP1-32681] - Human colon cancer. IL1 Receptor 2 antibody [N3C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/IL-1-RII-Antibody-Immunohistochemistry-Paraffin-NBP1-32681-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>LoVo whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IL-1 RII antibody [N3C3] (NBP1-32681) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32681_rabbit-polyclonal-il-1-rii-antibody-western-blot-141120251829416.jpg)
![LoVo whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IL1 Receptor 2 antibody [N3C3] (NBP1-32681) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32681_rabbit-polyclonal-il-1-rii-antibody-western-blot-261120251854117.jpg)
MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # 
263-2R) was serially diluted and captured by Mouse Anti-Human IL‑1 RII Monoclonal Antibody (Catalog # MAB663) coated on a Clear Polystyrene Microplate (Catalog # 
