MHC class II (I-A/I-E) Antibody (M5/114.15.2) [PE]

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Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [PE] [NBP1-43767] - See Notes

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
M5/114.15.2
Clonality
Monoclonal
Host
Rat
Conjugate
PE
Concentration
0.2 mg/ml

Order Details

MHC class II (I-A/I-E) Antibody (M5/114.15.2) [PE] Summary

Immunogen
The immunogen for this antibody was MHC Class II.
Specificity
The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
H2-Ea-ps
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions and can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

Use in Human reported in scientific literature (PMID:29990503).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS (pH 7.2) with 0.1% gelatin
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for MHC class II (I-A/I-E) Antibody (M5/114.15.2) [PE]

  • H2-Ea
  • MHC class II (I-A/I-E)
  • MHC class II IA/IE

Background

The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u but not from mice carrying the H-2s or H-2f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-43312AF350
Alexa Fluor 405 NBP1-43312AF405
Alexa Fluor 488 NBP1-43312AF488
Alexa Fluor 532 NBP1-43312AF532
Alexa Fluor 594 NBP1-43312AF594
Alexa Fluor 647 NBP1-43312AF647
Alexa Fluor 700 NBP1-43312AF700
Alexa Fluor 750 NBP1-43312AF750
Allophycocyanin NBP1-43312APC
Allophycocyanin NBP1-42997
Allophycocyanin/Cy7 NBP1-43312APCCY7
Biotin NBP1-43552
Biotin NBP1-43312B
Biotin NBP1-43552
DyLight 350 NBP1-43312UV
DyLight 405 NBP1-43312V
DyLight 488 NBP1-43312G
DyLight 550 NBP1-43312R
DyLight 594 NBP1-43312DL594
DyLight 650 NBP1-43312C
DyLight 680 NBP1-43312FR
DyLight 755 NBP1-43312IR
FITC NBP1-43312F
FITC NBP1-43950
FITC NBP1-43950
HRP NBP1-43312H
Janelia Fluor 549 NBP1-43312JF549
Janelia Fluor 646 NBP1-43312JF646
PE NBP1-43767
PE NBP1-43767
PE NBP1-43767
PE NBP1-43312PE
PE/Atto594 NBP1-43312PEATT594
PE/Cy5 NBP1-43070
PE/Cy5 NBP1-43070
PE/Cy5.5 NBP1-43312PECY55
PE/Cy7 NBP1-43312PECY7
PerCP NBP1-43312PCP

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Bioinformatics

Gene Symbol H2-Ea-ps