MHC Class II Antibody (ER-TR2)

Images

 
Immunocytochemistry/ Immunofluorescence: MHC Class II Antibody (ER-TR2) [NB100-64959] - Staining of MHC II in conjunctiva associated lymphoid tissue in mice reared in a pathogen free environment for the purposed of ...read more
Immunocytochemistry/ Immunofluorescence: MHC Class II Antibody (ER-TR2) [NB100-64959] - Comparison of MHC II levels in retinal microglia from mice without transplanted GFP+ BM Cells (panel A) and from mice with ...read more
Immunocytochemistry/ Immunofluorescence: MHC Class II Antibody (ER-TR2) [NB100-64959] - Staining of MHC II in conjunctiva associated lymphoid tissue in mice kept in standard living conditions for the purpose of ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr
Clone
ER-TR2
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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Catalog# & Conjugate Size Price

MHC Class II Antibody (ER-TR2) Summary

Immunogen
Isolated C3H thymic stromal cells
Localization
Type I membrane protein. Localizes to late endocytic compartment. Associates with lysosome membranes.
Specificity
NB100-64959 recognizes a polymorphic determinant on the murine MHC (major histocompatibility complex) class II molecule. In the mouse, MHC class II molecules are expressed by dendritic cells, B-cells and macrophages. In thymus tissues, clone ER-TR2 stains cortical and medullary stromal cells. Clone ER-TR2 specifically recognizes mouse strains with the haplotypes I-A k,s,r. Mouse strains with the haplotypes I-A b,d,q,f are not recognized by this antibody.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
HLA-DMA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100-1:200
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
Use in ICC/IF was reported in scientific literature (PMID: 23750207).
Publications
Read Publications using
NB100-64959 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for MHC Class II Antibody (ER-TR2)

  • class II histocompatibility antigen, M alpha chain
  • D6S222E
  • DMA
  • HLA class II histocompatibility antigen, DM alpha chain
  • major histocompatibility complex, class II, DM alpha
  • MHC class II antigen DMA
  • Really interesting new gene 6 protein
  • RING6HLADM

Background

MHC Class II antigens are heterodimers consisting of one alpha chain(31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MHC Class II Antibody (NB100-64959)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-Fr.


Filter By Application
ICC/IF
(2)
IHC
(1)
IHC-Fr
(1)
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Mouse
(2)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for MHC Class II Antibody (NB100-64959) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 350 NB100-64959UV
DyLight 405 NB100-64959V

Additional MHC Class II Products

Array NB100-64959

Bioinformatics Tool for MHC Class II Antibody (NB100-64959)

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Blogs on MHC Class II.

CD74 - a central player in antigen presentation by MHC class II
Cluster of differentiation 74 (CD74) is an important integral membrane protein that serves as a chaperone for MHC class II molecules. CD74, also known as the invariant chain or Ii, is needed for the proper folding and trafficking of MHC class II in...  Read full blog post.

Lymphocyte activation gene 3 (LAG3): No lag time in immune response
The LAG3 protein belongs to the Ig superfamily and contains 4 extracellular Ig-like domains (D1-D4). This molecule plays an key role in the immune response through negative regulation of T-cell proliferation, function, and homeostasis. It is required...  Read full blog post.

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Bioinformatics

Gene Symbol HLA-DMA
Entrez