MGC20410 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: MGC20410 Antibody [NBP2-38963] - Staining of human testis shows strong positivity in nucleoli in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: MGC20410 Antibody [NBP2-38963] - Staining of human kidney shows strong positivity in nucleoli in cells in tubules.

Immunocytochemistry/Immunofluorescence: MGC20410 Antibody [NBP2-38963] - Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoli.

Immunohistochemistry-Paraffin: MGC20410 Antibody [NBP2-38963] - Staining of human colon shows nucleoli positivity in glandular cells.

Immunohistochemistry-Paraffin: MGC20410 Antibody [NBP2-38963] - Staining of human colon shows strong positivity in nucleoli in glandular cells.

Immunohistochemistry-Paraffin: MGC20410 Antibody [NBP2-38963] - Staining of human Fallopian tube shows strong positivity in nucleoli in glandular cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MGC20410 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MGC20410 Antibody - BSA Free (NBP2-38963) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: MHLCGGNGLLTQTDPKEQQRQLKKQKNRAAA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BATF2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  MGC20410 Protein (NBP2-38963PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MGC20410 Antibody - BSA Free

• basic leucine zipper transcription factor, ATF-like 2
• basic leucine zipper transcriptional factor ATF-like 2
• B-ATF-2
• MGC20410
• SARI
• suppressor of AP-1 regulated by IFN

Background

BATF2 (Basic leucine zipper transcriptional factor ATF-like 2) is a 274 amino acid member of the AP-1 transcription factor family. It controls the differentiation of lineage-specific cells in the immune system. It participates in differentiating CD8+ thymic conventional dendritic cells and acts by forming a heterodimer with JUN family proteins. They then recognize and bind the DNA sequence 5'-TGA(CG)TCA-3'. It acts as a tumor suppressor by interfering with AP-1 regulated activation of CYR61/CCN1 transcription and its downstream signaling. CYR61/CCN1 signaling induces anchorage-independent growth and invasion in several cancer types, such as breast cancer, malignant glioma and metastatic melanoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BATF2
• Uniprot:
  • Q8N1L9(Human)