MGAT1 Antibody

Images

 
Western Blot: MGAT1 Antibody [NBP1-52077] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MGAT1 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MGAT1 Antibody Summary

Immunogen
Peptide with sequence C-QVEKVRTNDR corresponding to internal region according to NP_002397.2.
Epitope
C-QVEKVRTNDR
Specificity
Reported variants represent identical protein: NP_002397.2, NP_001108090.1, NP_001108091.1, NP_001108092.1, NP_001108089.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MGAT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 - 1 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: In transfected HEK293 transiently expressing full-length human MGAT1 (myc and DYKDDDDK tagged), a band of approx. 55 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody.
Publications
Read Publication using NBP1-52077.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MGAT1 Antibody

  • alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
  • EC 2.4.1.101
  • GGNT1
  • GlcNAc-T I
  • GlcNac-TI
  • GLCT1
  • GLYT1GLCNAC-TI
  • GNT-1
  • GNT-I
  • mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
  • MGAT
  • MGAT1
  • N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I

Background

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MGAT1 Antibody (NBP1-52077)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MGAT1
Entrez
Uniprot