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MFF Antibody

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Western Blot: MFF Antibody [NBP2-59110] - Detection of human MFF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T (50ug) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MFF antibody NBP2-59110 used for IP at 20 ul per reaction. MFF was also immunoprecipitated by rabbit anti-MFF antibody NBP2-59109. For blotting immunoprecipitated MFF,NBP2-59110 was used at 1:250. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: MFF Antibody [NBP2-59110] - Detection of human MFF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T (50ug) cells prepared using ...read more
Western Blot: MFF Antibody [NBP2-59110] - Detection of human and mouse MFF by western blot. Samples: Whole cell lysate from HeLa (5 and 15 ug) 293T, (50ug), and Jurkat (50ug) cells prepared using NETN lysis buffer. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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MFF Antibody Summary

Immunogen
Maps to a region between 50 and 100
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MFF
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 20 ul/mg lysate
  • Western Blot 1:1000

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
Tris-buffered Saline containing 0.1% BSA
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MFF Antibody

  • mitochondrial fission factor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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DRP1 Antibody -
BSA Free
NB110-55288
Flow Cytometry: DRP1 Antibody [NB110-55288] - An intracellular stain was performed on HeLa cells with NB110-55288 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.
Immunocytochemistry/Immunofluorescence: DRP1 Antibody [NB110-55288] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-DRP1 at 5 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/Immunofluorescence: DRP1 Antibody [NB110-55288] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DRP1 Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
(34 Publications)
Species: Fi, Hu, Mu, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
CRMP1 Antibody
(JU73-30)
NBP2-75449
Flow Cytometry: CRMP1 Antibody (JU73-30) [NBP2-75449] - Analysis of SH-SY-5Y cells with CRMP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: CRMP1 Antibody (JU73-30) [NBP2-75449] - Staining CRMP1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: CRMP1 Antibody (JU73-30) [NBP2-75449] - Staining CRMP1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Utrophin Antibody
NBP1-84074
Immunohistochemistry-Paraffin: Utrophin Antibody [NBP1-84074] - Staining of human prostate shows moderate to strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Utrophin Antibody [NBP1-84074] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli and cells in tubules.
Immunohistochemistry-Paraffin: Utrophin Antibody [NBP1-84074] - Staining of human stomach shows moderate membranous positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
DAP Kinase 2
Antibody (OTI1C5)
NBP2-02477
Flow Cytometry: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Analysis of Jurkat cells, using anti-DAP Kinase 2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DAP Kinase 2 antibody, and then analyzed by flow cytometry.
Immunocytochemistry/Immunofluorescence: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DAP Kinase 2.
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
DENR Antibody
NBP2-13913
Western Blot: DENR Antibody [NBP2-13913] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunohistochemistry-Paraffin: DENR Antibody [NBP2-13913] - Staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Western Blot: DENR Antibody [NBP2-13913] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-159
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
TTC11 Antibody
NB100-56646
Western Blot: TTC11 Antibody [NB100-56646] - Effect of liraglutide on brain cortical mitochondrial fission/fusion markers in female mice with early AD-like pathology. Brain cortical Fis1 (A) protein levels were determined and normalized to beta-actin levels, and representative Western blotting images displayed. Data are the mean +/- SE from 6 mice/group. Statistical significance: * p < 0.05, by the one-way ANOVA with the Fisher LSD post-hoc test for multiple comparisons. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/21/5/1746), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: TTC11 Antibody [NB100-56646] - Analysis of methcarn-fixed, paraffin-embedded hippocampus from control (normal) and Alzheimer brain. Quantification in five AD brains and five age-matched controls indicated that Fis1 was detected in only the soma in ~80% of pyramidal neurons in AD hippocampus, significantly different from what was seen for control hippocampal neurons. Source: Wang et al, 2009.
Knockdown Validated: TTC11 Antibody [NB100-56646] - Analysis of Fis1 in A) human HeLa and B) mouse NIH 3T3 lysate using Fis1 antibody at 1:1000 dilution. B. Specificity validation of the Fis1 antibody. Fis1 was analyzed in M17 cells stably transfected with vector only, Fis1 RNAi, Fis1 (Fis1wt OE) or left untransfected (negative control). The western blot data showed that M17 cells expressed Fis1 (Vector, Untransfected control) which could be knocked down by Fis1 RNAi. Cells transfected with Fis1 overexpressed Fis1. M17 is a human neuroblastoma cell line.
(26 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, WB
Mitofusin 2
Antibody - BSA Free
NBP2-66383
Immunohistochemistry-Paraffin: Mitofusin 2 Antibody [NBP2-66383] - Analysis of FFPE tissue section of mouse heart using Mitofusin 2/MFN2 antibody at 5 ug/mL concentration (1:200 dilution). The primary antibody binding to MFN2 antigen was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This MFN2 antibody generated a diffused to punctate cytoplasmic staining of mitochondria in the cardiac muscles (brown color). The nuclei of the muscle cells and the macrophages are stained blue in this image (40X image of a transverse section of heart tissue).
Immunocytochemistry/Immunofluorescence: Mitofusin 2 Antibody [NBP2-66383] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Mitofusin 2 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: Mitofusin 2 Antibody [NBP2-66383] - Total protein from human HeLa and A431, mouse Neuro2A and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-MFN2 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
SMCR7L Antibody
NBP1-59480
Western Blot: SMCR7L Antibody [NBP1-59480] - HT1080 cell lysate, concentration 0.2-1 ug/ml.
Western Blot: SMCR7L Antibody [NBP1-59480] - Human Fetal Heart.
Western Blot: SMCR7L Antibody [NBP1-59480] - Human Fetal Lung.
Species: Hu
Applications: WB
Cytochrome c
Antibody (7H8.2C12) - ...
NB100-56503
Flow Cytometry: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Using the Alexa Fluor 488 direct conjugate, an intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow (Intracellular): Cytochrome c Antibody (7H8.2C12) [NB100-56503] - An intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Metformin activates apoptotic cell death; Right panel: MCF-7 cells were kept in the presence or absence of metformin for 20 days and then processed to obtain mitochondrial or whole cellular extracts. BAX and CYCS expression levels were determined by Western blot as indicated under Material and Methods. Densitometric analysis of the gels was performed as indicated under Material and Methods. PHB and CDK4 were used as purity and loading controls. Image collected and cropped by CiteAb from the following publication (http://www.mdpi.com/2073-4409/8/1/49), licensed under a CC-BY licence.
(58 Publications)
Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
TNF-alpha
[Unconjugated]
210-TA/CF
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA/CF) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
(702 Publications)
Species: Hu
Applications: BA
REEP4 Antibody
NBP2-34081
Western Blot: REEP4 Antibody [NBP2-34081] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: REEP4 Antibody [NBP2-34081] - Staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.
Species: Hu
Applications: IHC, IHC-P, WB
OPA1 Antibody -
BSA Free
NB110-55290
Western Blot: OPA1 Antibody [NB110-55290] - Depletion of Oma1 in fish affects development. Immunoblot of protein extracts from injected fish (yolk removed) at 24, 48 and 72 hpf. Expression of Oma1, Opa1 isoforms (a-f) and Cox4 (mitochondrial abundance marker) was analyzed with respective antibodies. Actin and Ponceau S staining were loading controls. Source data (full-length blots) are available online in Supplementary information. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep13989), licensed under a CC-BY licence.
Western Blot: OPA1 Antibody [NB110-55290] - Detection of OPA1 protein in post-nuclear extracts of mouse embryonic fibroblasts.
Simple Western: OPA1 Antibody [NB110-55290] - Lane view shows a specific band for OPA1 in 0.5 mg/ml of MEF lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
(36 Publications)
Species: Ch, Hu, Mu, Po, Rt, Ze
Applications: IB, ICC/IF, IHC, IHC-P, Simple Western, WB
Dynamin Antibody
(3G4B6)
NB110-60491
Knockout Validated: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot using lysates from U20S parental cell line and Dynamin knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Dynamin Monoclonal Antibody (Catalog # NB110-60491) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Dynamin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Immunoprecipitation: Dynamin Antibody (3G4B6) [NB110-60491] - Antibody-bead conjugates were prepared by adding 1.0 ug of antibody to 500 ul of PBS with 0,01% triton X-100 in a microcentrifuge tube and 30ul of protein G Sepharose beads. Tubes were rocked O/N at 4 degrees C followed by several washes to remove unbound antibodies. U2OS were collected in HEPES buffer (20 mM HEPES, 100 mM sodium chloride, 1 mM EDTA, 1% Triton X-100, pH 7.4) supplemented with protease inhibitor. Lysates are rocked 30 min at 4 degrees C and spun at 110,000xg for 15 min at 4 degrees C. One ml aliquots at 1 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4 degrees C. Following centrifugation, the unbound fractions were collected, and beads were subsequently washed three times with 1 ml of HEPES lysis buffer and processed for SDS-PAGE and immunoblot. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot analysis using Dynamin mouse mAb against C6 (1), NIH/3T3 (2), SKN-SH (3), LN18 (4), SHSY5Y (5) cell lysate and rat brain tiisues lysate (6).
Species: Hu, Mu
Applications: ELISA, Flow, IHC, IHC-Fr, IHC-P, IP, KO, Simple Western, WB
MED12 Antibody
NB100-2357
Immunohistochemistry-Paraffin: MED12 Antibody [NB100-2357] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MED12 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: MED12 Antibody [NB100-2357] - Detection of human MED12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MED12 antibody NB100-2357 used for IP at 20 ul/mg lysate. MED12 was also immunoprecipitated by rabbit anti-MED12 recombinant monoclonal antibody [BLR084G] (NBP2-80138). For blotting immunoprecipitated MED12, NBP2-80138 was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry: MED12 Antibody [NB100-2357] - Representative examples of immunohistochemically stained sections positive for MED12 (A, B and C) in tumor specimens. Examples are from the same patients, respectively. A. TS = 5, B. TS = 6, C. TS = 6 (x20 original magnification [A, B and C]. Scale bars represent 100 um. TS, total score = intensity score (0-3) plus proportion score (0-3). Image collected and cropped by CiteAb from the following publication (http://www.oncotarget.com/abstract/14410), licensed under a CC-BY licence.
(13 Publications)
Species: Hu, Mu, Pm
Applications: ChIP, IHC, IHC-P, IP, WB
Mitofusin 1
Antibody (3C9)
H00055669-M04
Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - MFN1 monoclonal antibody (M04), clone 3C9 Western Blot analysis of MFN1 expression in HeLa ( Cat # L013V1 ).
Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Western blot analysis of MFN1 over-expressed 293 cell line, cotransfected with MFN1 Validated Chimera RNAi or non-transfected control. Blot probed with H00055669-M04. GAPDH (36.1 kDa) used as loading control.
Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Analysis of MFN1 expression in transfected 293T cell line by MFN1 monoclonal antibody (M04), clone 3C9. Lane 1: MFN1 transfected lysatE (84.2 KDa). Lane 2: Non-transfected lysate.
(26 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IP, KD, WB
Myelin Protein
Zero Antibody
NB100-1607
Immunohistochemistry: Myelin Protein Zero Antibody [NB100-1607] - In the image (a lower power tissue section through an adult sciatic nerve), Myelin Protein Zero (brown staining) can be seen in all of the myelinating Schwann cells.
Immunocytochemistry/Immunofluorescence: Myelin Protein Zero Antibody [NB100-1607] - Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of an adult mouse. (Green) P0 concentration was 1:1000 dilution, followed by fluorescein-labeled goat anti-chicken IgY (1:500 dilution). Red is actin. Blue is DAPI nuclear counterstain.
(8 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IP, WB
NHE1/SLC9A1
Antibody
NBP2-38683
Immunocytochemistry/Immunofluorescence: NHE1/SLC9A1 Antibody [NBP2-38683] - Staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP2-38683] - Staining of human kidney shows moderate membranous/cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP2-38683] - Staining of human liver shows no positivity in hepatocytes as expected.
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
ROS Antibody
(OTI1F3)
NBP2-45626
Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626] - Analysis of Carcinoma Human lung tissue.
Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626] - Analysis of Carcinoma Human lung tissue.
Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626] - Analysis of Human carcinoma lung tissue.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
MLKL [p Ser345]
Antibody (JM92-37)
NBP2-66953
Immunohistochemistry-Paraffin: MLKL [p Ser345] Antibody (JM92-37) [NBP2-66953] - Analysis of paraffin-embedded mouse colon tissue using anti-MLKL (phospho S345) antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MLKL [p Ser345] Antibody (JM92-37) [NBP2-66953] - Analysis of paraffin-embedded mouse liver tissue using anti-MLKL (phospho S345) antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MLKL [p Ser345] Antibody (JM92-37) [NBP2-66953] - Analysis of paraffin-embedded mouse lung tissue using anti-MLKL (phospho S345) antibody. Counter stained with hematoxylin.
(5 Publications)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
GDAP1 Antibody
NBP1-84430
Immunocytochemistry/Immunofluorescence: GDAP1 Antibody [NBP1-84430] - Immunofluorescent staining of human cell line A549 shows localization to cytosol & mitochondria. Antibody staining shown in green.
Immunohistochemistry-Paraffin: GDAP1 Antibody [NBP1-84430] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: GDAP1 Antibody [NBP1-84430] - Staining of human pancreas shows low expression as expected.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB

Publications for MFF Antibody (NBP2-59110) (0)

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Bioinformatics

Gene Symbol MFF