Methionyl tRNA synthetase Antibody (5G5) - Azide and BSA Free

Western Blot: Methionyl tRNA synthetase Antibody (5G5) [H00004141-M01] - MARS monoclonal antibody (M01), clone 5G5 Analysis of MARS expression in HepG2.

Immunocytochemistry/Immunofluorescence: Methionyl tRNA synthetase Antibody (5G5) [H00004141-M01] - Analysis of monoclonal antibody to MARS on HepG2 cell. Antibody concentration 10 ug/ml.

Sandwich ELISA: Methionyl tRNA synthetase Antibody (5G5) [H00004141-M01] - Detection limit for recombinant GST tagged MARS is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 5G5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Methionyl tRNA synthetase Antibody (5G5) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MARS (NP_004981, 801 a.a. ~ 899 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LFQKLENDQIESLRQRFGGGQAKTSPKPAVVETVTTAKPQQIQALMDEVTKQGNIVRELKAQKADKNEVAAEVAKLLDLKKQLAVAEGKPPEAPKGKKK
• Localization: Cytoplasmic
• Specificity: MARS - methionine-tRNA synthetase
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MARS1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Methionyl tRNA synthetase Antibody (5G5) - Azide and BSA Free

• cytosolic methionyl-tRNA synthetase
• EC 6.1.1.10
• FLJ35667
• methionine tRNA ligase 1, cytoplasmic
• Methionine--tRNA ligase
• methionyl-tRNA synthetase
• methionyl-tRNA synthetase, cytoplasmic
• MetRS
• MRS
• MTRNS

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene belongs to the class I family of tRNA synthetases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MARS1
• Entrez:
  • 4141(Human)
• OMIM:
  • 156560(Human)
• Uniprot:
  • NP_004981(Human)