Melanocortin-2 R/MC2R Overexpression Lysate (Native)

Images

 
Western Blot: MC2 Receptor Overexpression Lysate (Adult Normal) [NBP2-04160] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MC2 Receptor.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Melanocortin-2 R/MC2R Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_000520. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens MC2 Receptor, mRNA.
Gene
MC2R

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
33.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-04160 in the following application:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Melanocortin-2 R/MC2R

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Melanocortin-2 R/MC2R Overexpression Lysate (Native)

  • ACTH receptor
  • ACTHR
  • ACTH-R
  • ACTHRcorticotropin receptor
  • adrenocorticotropic hormone receptor
  • Adrenocorticotropin receptor
  • MC2 receptor
  • MC2R
  • MC2-R
  • melanocortin 2 receptor (adrenocorticotropic hormone)
  • Melanocortin receptor 2
  • Melanocortin-2 R
  • Melanocortin2R
  • Melanocortin-2R
  • MGC125798

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Melanocortin-2 R/MC2R Lysate (NBP2-04160) (0)

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Review for Melanocortin-2 R/MC2R Lysate (NBP2-04160) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-04160:
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Images Ratings Applications Species Date Details
Western Blot Melanocortin-2 R/MC2R NBP2-04160
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reviewed by:
Anonymous
WB Human 11/19/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate
SpeciesHuman
Lot4160

Blocking

Blocking Details5% milk solution

Primary Anitbody

Dilution Ratio1:1000, 1 hour Incubation at RT

Secondary Antibody

Secondary DescriptionGoat anti-rabbit HRP Conjugate
Secondary Concentration1:1000

Details

Detection NotesDensitometry, VersaDoc MP 5000, 30 seconds

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Video Protocols

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FAQs for Melanocortin-2 R/MC2R Lysate (NBP2-04160) (0)

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Recent Reviews

5
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1
4
0
3
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2
0
1
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Anonymous
11/19/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol MC2R
Entrez